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2.7.1.33: pantothenate kinase

This is an abbreviated version!
For detailed information about pantothenate kinase, go to the full flat file.

Word Map on EC 2.7.1.33

Reaction

ATP
+
(R)-pantothenate
=
ADP
+
(R)-4'-phosphopantothenate

Synonyms

4-phosphopantoate, BaPanK, Cab1, Cab1p, CoaA, coaW, CoaX, D-pantothenate kinase, EhPAnK, fumble, hPanK, hPanK1, hPANK2, hPanK3, hPanK4, HpPanK-III, HsPANK3, HsPANK4, kinase, pantothenate (phosphorylating), More, mPank, mPank1, mPanK2, mPanK3, MtCoaA, MtPanK, PAK, PanK, PanK-III, PanK1, PanK1alpha, PanK1b, PanK2, PanK3, PanK4, PanKBa, pantothenate kinase, pantothenate kinase 1, pantothenate kinase 2, pantothenate kinase 3, pantothenate kinase 4, pantothenate kinase-2, pantothenic acid kinase, PfPanK, Pfpank1, PoK, rPanK4, Rts protein , Rv1092c, TK2141, TmPanK-III, type II pantothenate kinase, type III pantothenate kinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.33 pantothenate kinase

Purification

Purification on EC 2.7.1.33 - pantothenate kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-tagged PanK2
HiTrap chelating HP column chromatography and Superdex 200 column gel filtration
immobilized Co2+-affinity chromatography
native enzyme partially by preparation of mitochondria
Ni-column chromatography
-
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex pg 200 gel filtration
-
Ni-NTA metal affinity column chromatography
Ni-NTA resin column chromatography and Superdex 200 gel filtration
Ni-NTA resin column chromatography and Superdex 75 gel filtration
nickel-nitrilotriacetic acid-agarose column chromatography and Resource Q column chromatography
-
nickel-Sepharose column chromatography and Superdex 75 gel filtration
partial
partial, presumably 2 isoforms
-
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, desalting gel filtration, anion exchange chromatography, gel filtration, and ultrafiltration
A0A167Z3Z6
recombinant enzyme from strain BL21(DE3) by ion exchange and hydrophobic interaction chromatography, and gel filtration, recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography
recombinant enzyme, six-His-tagged
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) cells by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis
recombinant His/myc-tagged isozyme PanK2 from mitochondria of QBI 293 cells by ion exchange and protein A affinity chromatography and gel filtration
-
recombinant His6-tagged from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
strain SJ16, partial
-