2.7.1.35: pyridoxal kinase

This is an abbreviated version!
For detailed information about pyridoxal kinase, go to the full flat file.

Reaction

Synonyms

ePL kinase, ePL kinase 1, hPL kinase, HPLK, kinase (phosphorylating), pyridoxal, kinase, pyridoxal (phosphorylating), LdPdxK, PdxK, pdxY, PKH, PL kinase, PLK, Plk1, PM kinase, PN kinase, PN/PL/PM kinase, pyridoxal 5-phosphate-kinase, pyridoxal kinase, pyridoxal kinase 1, pyridoxal kinase PKL, pyridoxal kinase-like protein SOS4, pyridoxal phosphokinase, pyridoxal/pyridoxine kinase, pyridoxamine kinase, pyridoxine kinase, pyridoxine/pyridoxal/pyridoxamine kinase, salt overly sensitive4, SAV0580, Sos4, StPLK, vitamin B6 kinase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.35 pyridoxal kinase

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Results	in table
4	Activating Compound
18327	AA Sequence
6	Application
1	CAS Registry Number
32	Cloned(Commentary)
10	Cofactor
18	Crystallization (Commentary)
88	Disease/ Diagnostics
1	Expression
25	General Information
5	General Stability
5	IC50 Value
129	Inhibitors
26	kcat/KM [mM/s]
16	Ki Value [mM]
122	KM Value [mM]
6	Localization
96	Metals/Ions
25	Molecular Weight [Da]
40	Natural Substrates/ Products (Substrates)
137	Organism
7	Pathway
98	PDB ID
22	pH Optimum
6	pH Range
1	pH Stability
6	pI Value
26	Protein Variants
45	Purification (Commentary)
2	Reaction
1	Reaction Type
94	Reference
1	Renatured (Commentary)
54	Source Tissue
29	Specific Activity [micromol/min/mg]
4	Storage Stability
191	Substrates and Products (Substrate)
32	Subunits
67	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
69	Turnover Number [1/s]

KM Value

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KM Value on EC 2.7.1.35 - pyridoxal kinase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0216

4'-deoxypyridoxine

2 entries

0.00495

4'-O-methylpyridoxine

Homo sapiens

O00764

pH 6.2, 37°C

673580

1.85 - 2.03

4-Amino-5-hydroxymethyl-2-methylpyrimidine

3 entries

0.0091 - 3.096

ATP

40 entries

0.00495 - 0.0466

ginkgotoxin

3 entries

0.00122 - 3.839

pyridoxal

42 entries

0.006 - 0.1818

pyridoxamine

10 entries

0.00172 - 2.07

pyridoxine

17 entries

additional information

additional information

4 entries

-

0.0216

4'-deoxypyridoxine

Homo sapiens

O00764

pH 6.2, 37°C

673580

0.0216

4'-deoxypyridoxine

Homo sapiens

O00764

in 70 mM potassium phosphate buffer, pH 6.2

673580

1.85

4-Amino-5-hydroxymethyl-2-methylpyrimidine

Staphylococcus aureus

A0A0H3JTP0

mutant C110A, pH not specified in the publication, temperature not specified in the publication

738451

1.99

4-Amino-5-hydroxymethyl-2-methylpyrimidine

Staphylococcus aureus

A0A0H3JTP0

wild-type, pH not specified in the publication, temperature not specified in the publication

738451

2.03

4-Amino-5-hydroxymethyl-2-methylpyrimidine

Bacillus subtilis

-

pH 8, 37°C

662007

0.0091

ATP

Trypanosoma brucei

-

pH 7, 37°C

641426

0.012

ATP

Sus scrofa

-

pH 6.0, 37°C

641418

0.012

ATP

Homo sapiens

-

pH 7, 37°C

641425

0.02

ATP

Ovis aries

-

pH 6.5

641413

0.02

ATP

Homo sapiens

-

in the presence of Zn2+, at pH 6.5 and 37°C

737809

0.025

ATP

Homo sapiens

O00764

in the presence of K+, and in complex with Mg2+

677029

0.025

ATP

Homo sapiens

O00764

presence of 40 mM Na+, pH 7.3, 37°C

677029

0.0579

ATP

Bombyx mori

Q1PCB1

in 70 mM potassium phosphate (pH 5.5), 0.5 mM ZnCl2, at 37°C

722183

0.06

ATP

Ovis aries

-

pH 5.8, 37°C

641419

0.06

ATP

Ovis aries

-

ATP in form of ZnATP2-

641419

0.07

ATP

Escherichia coli

-

kinase 1 with pyridoxal as substrate, pH 7.3, 37°C

663303

0.07

ATP

Escherichia coli

-

ATP in form of ZnATP2-

663303

0.078

ATP

Pseudomonas aeruginosa

-

mutant enzyme C124A, at pH 7.4 and 37°C

737622

0.082

ATP

Plasmodium falciparum

-

pH 6.5, 30°C

662440

0.1

ATP

Salmonella enterica subsp. enterica serovar Typhimurium

A0A0F7J8S0

with pyridoxal, pH 6.6, 37°C

759183

0.101

ATP

Ovis aries

-

pH 5.8, 37°C

641419

0.101

ATP

Ovis aries

-

ATP in form of MgATP2-

641419

0.102

ATP

Pseudomonas aeruginosa

-

wild type enzyme, at pH 7.4 and 37°C

737622

0.12

ATP

Salmonella enterica subsp. enterica serovar Typhimurium

A0A0F7J8S0

with pyridoxamine, pH 8.5, 37°C

759183

0.127

ATP

Trypanosoma brucei

-

pH 7, 37°C, recombinant enzyme

641426

0.17

ATP

Homo sapiens

O00764

D235A mutant protein

702038

0.17

ATP

Homo sapiens

O00764

ATP in form of MgATP

702038

0.18

ATP

Homo sapiens

O00764

D235N mutant protein

702038

0.18

ATP

Homo sapiens

O00764

ATP in form of MgATP

702038

0.19

ATP

Homo sapiens

O00764

wild-type protein

702038

0.19

ATP

Homo sapiens

O00764

ATP in form of MgATP

702038

0.194

ATP

Ovis aries

-

pH 5.8, 37°C

641419

0.194

ATP

Ovis aries

-

ATP in form of MnATP2-

641419

0.2

ATP

Leishmania donovani

A0A0G2YFI9

recombinant wild-type enzyme, pH 7.2, 37°C

759342

0.377

ATP

Homo sapiens

O00764

pH 7.3, 37°C recombinant mutant D87H

760178

0.407

ATP

Homo sapiens

O00764

pH 7.3, 37°C recombinant wild-type enzyme

760178

0.42

ATP

Homo sapiens

O00764

with pyridoxal as substrate, pH 7.3, 37°C

663303

0.42

ATP

Homo sapiens

O00764

ATP in form of MgATP

663303

0.5

ATP

Homo sapiens

O00764

in the presence of Na+, and in complex with Mg2+

677029

0.5

ATP

Homo sapiens

O00764

presence of 40 mM Na+, pH 7.3, 37°C

677029

0.6

ATP

Escherichia coli

-

kinase 1 with pyridoxal as substrate, pH 7.3, 37°C

663303

0.6

ATP

Escherichia coli

-

ATP in form of MgATP2-

663303

0.901

ATP

Homo sapiens

O00764

pH 7.3, 37°C recombinant mutant H246Q

760178

1.024

ATP

Homo sapiens

O00764

pH 7.3, 37°C recombinant mutant A243G

760178

3.096

ATP

Homo sapiens

O00764

pH 7.3, 37°C recombinant mutant V128I

760178

0.00495

ginkgotoxin

Homo sapiens

O00764

in 70 mM potassium phosphate buffer, pH 6.2

673580

0.0297

ginkgotoxin

Leishmania donovani

A0A0G2YFI9

recombinant enzyme, pH 7.5, 37°C

759346

0.0466

ginkgotoxin

Trypanosoma brucei

-

recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C

723219

0.00122

pyridoxal

Homo sapiens

-

in the presence of K+, at pH 7.0 and 30°C

738236

0.00165

pyridoxal

Trypanosoma brucei

-

recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C

723219

0.0028

pyridoxal

Homo sapiens

O00764

in 70 mM potassium phosphate buffer, pH 6.2

673580

0.01

pyridoxal

Homo sapiens

O00764

in the presence of K+

677029

0.01

pyridoxal

Homo sapiens

O00764

presence of 40 mM Na+, pH 7.3, 37°C

677029

0.0153

pyridoxal

Plasmodium vivax

A5K1Z8

in the presence of K+, at pH 7.0 and 30°C

738236

0.0216

pyridoxal

Trypanosoma cruzi

Q4D946

in the presence of K+, at pH 7.0 and 30°C

738236

0.022

pyridoxal

Trypanosoma brucei

-

pH 7, 37°C

641426

0.024

pyridoxal

Homo sapiens

O00764

wild-type protein

702038

0.025

pyridoxal

Sus scrofa

-

pH 6, 37°C

641418

0.03

pyridoxal

Homo sapiens

O00764

with MgATP2- as substrate, pH 7.3, 37°C

663303

0.033

pyridoxal

Homo sapiens

-

-

641406

0.037

pyridoxal

Pseudomonas aeruginosa

-

mutant enzyme C124A, at pH 7.4 and 37°C

737622

0.038

pyridoxal

Trypanosoma brucei

-

pH 7, 37°C, recombinant enzyme

641426

0.038

pyridoxal

Trypanosoma brucei

-

recombinant enzyme, apparent value, in 70 mM potassium phosphate pH 7.0, 0.15 mM ZnCl2, at 37°C

723219

0.04

pyridoxal

Ovis aries

-

pH 6.5

641413, 641417

0.044

pyridoxal

Salmonella enterica subsp. enterica serovar Typhimurium

A0A0F7J8S0

pH 6.6, 37°C

759183

0.0441

pyridoxal

Bombyx mori

Q1PCB1

in 70 mM potassium phosphate (pH 5.5), 0.5 mM ZnCl2, at 37°C

722183

0.047

pyridoxal

Bacillus subtilis

-

pH 8, 37°C

662007

0.053

pyridoxal

Pseudomonas aeruginosa

-

wild type enzyme, at pH 7.4 and 37°C

737622

0.058

pyridoxal

Homo sapiens

O00764

D235N mutant protein

702038

0.0587

pyridoxal

Homo sapiens

O00764

pH 6.2, 37°C

673580

0.07

pyridoxal

Plasmodium falciparum

-

pH 6.5, 30°C

662440

0.0739

pyridoxal

Homo sapiens

-

in the presence of Na+, at pH 7.0 and 30°C

738236

0.075

pyridoxal

Homo sapiens

O00764

in the presence of Na+

677029

0.075

pyridoxal

Homo sapiens

O00764

presence of 40 mM Na+, pH 7.3, 37°C

677029

0.0909

pyridoxal

Leishmania donovani

A0A0G2YFI9

recombinant enzyme, pH 7.5, 37°C

759346

0.097

pyridoxal

Homo sapiens

-

pH 7, 37°C

641425

0.1

pyridoxal

Escherichia coli

-

kinase 1 with MgATP2- as substrate, pH 7.3, 37°C

663303

0.111

pyridoxal

Staphylococcus aureus

A0A0H3JTP0

wild-type, pH not specified in the publication, temperature not specified in the publication

738451

0.141

pyridoxal

Homo sapiens

O00764

pH 7.3, 37°C recombinant mutant H246Q

760178

0.17

pyridoxal

Homo sapiens

O00764

D235A mutant protein

702038

0.177

pyridoxal

Homo sapiens

O00764

pH 7.3, 37°C recombinant mutant A243G

760178

0.189

pyridoxal

Homo sapiens

O00764

pH 7.3, 37°C recombinant wild-type enzyme

760178

0.19

pyridoxal

Rattus norvegicus

-

37°C

391873

0.19

pyridoxal

Escherichia coli

-

kinase 1 with ZnATP2- as substrate, pH 7.3, 37°C

663303

0.24

pyridoxal

Leishmania donovani

A0A0G2YFI9

recombinant wild-type enzyme, pH 7.2, 37°C

759342

0.26

pyridoxal

Staphylococcus aureus

A0A0H3JTP0

mutant C214D, pH not specified in the publication, temperature not specified in the publication

738451

0.35

pyridoxal

Homo sapiens

O00764

with ZnATP2- as substrate, pH 7.3, 37°C

663303

0.49

pyridoxal

Homo sapiens

-

at pH 6.5 and 37°C

737809

2.09

pyridoxal

Homo sapiens

O00764

pH 7.3, 37°C recombinant mutant D87H

760178

3.839

pyridoxal

Homo sapiens

O00764

pH 7.3, 37°C recombinant mutant V128I

760178

0.006

pyridoxamine

Ovis aries

-

pH 6.5

641417

0.0062

pyridoxamine

Homo sapiens

-

-

641406

0.01

pyridoxamine

Escherichia coli

-

kinase 1 with ZnATP2- as substrate, pH 7.3, 37°C

663303

0.03

pyridoxamine

Escherichia coli

-

kinase 1 with MgATP2- as substrate, pH 7.3, 37°C

663303

0.035

pyridoxamine

Homo sapiens

O00764

with MgATP2- as substrate, pH 7.3, 37°C

663303

0.0594

pyridoxamine

Trypanosoma brucei

-

recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C

723219

0.06

pyridoxamine

Salmonella enterica subsp. enterica serovar Typhimurium

A0A0F7J8S0

pH 8.5, 37°C

759183

0.126

pyridoxamine

Homo sapiens

O00764

pH 6.2, 37°C

673580

0.126

pyridoxamine

Homo sapiens

O00764

in 70 mM potassium phosphate buffer, pH 6.2

673580

0.1818

pyridoxamine

Leishmania donovani

A0A0G2YFI9

recombinant enzyme, pH 7.5, 37°C

759346

0.00172

pyridoxine

Homo sapiens

-

pH 7.0, 37°C

641415

0.0057

pyridoxine

Homo sapiens

-

-

641408

0.00987

pyridoxine

Homo sapiens

O00764

pH 6.2, 37°C

673580

0.00987

pyridoxine

Homo sapiens

O00764

in 70 mM potassium phosphate buffer, pH 6.2

673580

0.015

pyridoxine

Homo sapiens

O00764

with ZnATP2- as substrate, pH 7.3, 37°C

663303

0.016

pyridoxine

Homo sapiens

-

-

641406

0.017

pyridoxine

Sus scrofa

-

pH 6.0, 37°C

641418

0.02

pyridoxine

Homo sapiens

O00764

with MgATP2- as substrate, pH 7.3, 37°C

663303

0.025

pyridoxine

Escherichia coli

-

kinase 1 with MgATP2- as substrate, pH 7.3, 37°C

663303

0.026

pyridoxine

Ovis aries

-

pH 6.5

641417

0.048

pyridoxine

Trypanosoma brucei

-

recombinant enzyme, apparent value, in HEPES/MgCl2 pH 7.4, 0.15 mM ZnCl2, at 37°C

723219

0.06

pyridoxine

Ovis aries

-

pH 5.8, 37°C, with MnATP2- as the second substrate

641419

0.12

pyridoxine

Rattus norvegicus

-

37°C

391873

0.212

pyridoxine

Plasmodium falciparum

-

pH 6.5, 30°C

662440

0.4347

pyridoxine

Leishmania donovani

A0A0G2YFI9

recombinant enzyme, pH 7.5, 37°C

759346

1.51

pyridoxine

Staphylococcus aureus

A0A0H3JTP0

mutant C110A, pH not specified in the publication, temperature not specified in the publication

738451

2.07

pyridoxine

Staphylococcus aureus

A0A0H3JTP0

wild-type, pH not specified in the publication, temperature not specified in the publication

738451

additional information

additional information

Salmonella enterica subsp. enterica serovar Typhimurium

A0A0F7J8S0

steady-state kinetics

759183

-

additional information

additional information

Ovis aries

-

effect of KCl on Km-values

641419

-

additional information

additional information

Leishmania donovani

A0A0G2YFI9

Michaelis-Menten kinetics

759342

-

additional information

additional information

Leishmania donovani

-

Michaelis-Menten kinetics

759342

-