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2.7.1.43: glucuronokinase

This is an abbreviated version!
For detailed information about glucuronokinase, go to the full flat file.

Word Map on EC 2.7.1.43

Reaction

ATP
+
D-glucuronate
=
ADP
+
1-phospho-alpha-D-glucuronate

Synonyms

At3g01640, AtGlcAK, AtGlcAK2, GKUP, GlcAK, GlcAK1, GlcAK2, glucuronokinase, glucuronokinase 1, glucuronokinase 2, glucuronokinase, glucurono-, glucuronokinase1, kinase, glucurono- (phosphorylating)

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.43 glucuronokinase

Purification

Purification on EC 2.7.1.43 - glucuronokinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 688.5fold from pollen by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration followed by ultrafiltration, affinity chromatography, and another step of anion exchange chromatography, and hydrophobic interaction chromatography
-
partial purification
-
Protino-Ni1000 column chromatography and Strep-Tactin Macro Prep resin column chromatography
-
recombinant His-tagged enzyme from Escherichiaa coli strain XL-1 Blue by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain XL-1 Blue by nickel affinity chromatography