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2.7.1.48: uridine/cytidine kinase

This is an abbreviated version!
For detailed information about uridine/cytidine kinase, go to the full flat file.

Word Map on EC 2.7.1.48

Reaction

ATP
+
cytidine
=
ADP
 +
CMP

Synonyms

ATP:uridine 5'-phosphotransferase, hsUCK2, kinase, uridine (phosphorylating), More, pyrimidine ribonucleoside kinase, ttCK, UCK, UCK1, UCK2, UCKL1, Udk, UK/UPRT1, uridine cytidine kinase 2, uridine kinase, uridine phosphokinase, uridine-cytidine kinase, uridine-cytidine kinase 2, uridine/cytidine kinase, uridine/cytidine kinase 2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.48 uridine/cytidine kinase

Purification

Purification on EC 2.7.1.48 - uridine/cytidine kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Toyopearl Phenyl-650 M column chromatography, Toyopearl SP-650 column chromatography and Superdex 200 gel filtration
ammonium sulfate, Pb2+-precipitation
-
DE-52, ammonium sulfate, ATP-agarose column
-
DEAE-cellulose, ammonium sulfate, hydroxyapatite
-
immobilized metal ion adsorption chromatography (Ni2+)
Ni-NTA agarose column chromatography and Sephadex G25 gel filtration
partial purification
-
protamine sulfate, CM-cellulose, ammonium sulfate, DEAE-cellulose, zone electrophoresis
-
recombinant C-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography followed by ion-exchange chromatography
recombinant enzyme using glutathione-S-transferase tag
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
recombinant His-tagged uridine kinase, Ni-nitrilotriacetic acid-Sepharose
-
recombinant N-terminally His-tagged isozyme UCK1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant N-terminally His-tagged isozyme UCK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant protein using His-tag
-
recombinant ttCK from Escherichia coli strain Rosetta(DE3) by heat treatment at 60°C for 20 min, hydrophobic interaction chromatography, and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain Rosetta(DE3) by heat treatment at 70°C for 20 min, followed by hydrophobic interaction chromatography, and two different steps of gel filtration
streptomycin, ammonium sulfate, calcium phosphate, DEAE-cellulose
-
streptomycin, ammonium sulfate, calcium phosphate, Sephadex G-200
-
streptomycin, ammonium sulfate, DEAE-cellulose
-
ultracentrifugation, DEAE-cellulose, affinity chromatography on 5'amino-5'-deoxyuridine
-