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2.7.1.49: hydroxymethylpyrimidine kinase

This is an abbreviated version!
For detailed information about hydroxymethylpyrimidine kinase, go to the full flat file.

Word Map on EC 2.7.1.49

Reaction

ATP
+
4-Amino-5-hydroxymethyl-2-methylpyrimidine
=
ADP
+
4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine

Synonyms

4-amino-5-hydroxymethyl-2-methyl pyrimidine kinase, 4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate) kinase, 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase, 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase, EC 2.7.1.14, EC 2.7.1.35, HMP kinase, HMP-P kinase, HMP/HMP phosphate kinase, HMP/HMP-P kinase, HMPK, HMPPK, HVO_2666, hydroxymethylpyrimidine kinase (phosphorylating), hydroxymethylpyrimidine phosphate kinase, More, pyridoxal kinase, SAV0580, StHMPPK, Thi20, Thi20p, THI3, ThiD, TTHA0680, TtHMPPK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.49 hydroxymethylpyrimidine kinase

Cloned

Cloned on EC 2.7.1.49 - hydroxymethylpyrimidine kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA clone pBTH1 encoding bifunctional hydroxymethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase established in Escherichia coli DH5alpha, expressed in Escherichia coli NI500 and Salmonella typhimurium DM456 by functional complementation
coexpression of and functional complementation by His-tagged soluble Thi20p or Thi21p isozymes with a thiamin synthetase in an Escherichia coli strain SN372L-3-9-10 deficient in thiamin biosynthesis
-
Escherichia coli BL21(DE3) (pLysS) transformed with pTRD63 carrying ORF2, single gene thiD/J encodes 2 distinct enzyme activities, HMP kinase and HMP-P kinase, functional complemetation of Escherichia coli NI500, overexpression of ThiD
expressed in Escherichia coli
-
expressed in Escherichia coli strain BLR (DE3)
-
expression in Escherichia coli
gene annotated ThiD, instead of YjbV, subcloning in Escherichia coli strain DH5alpha, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene thiD, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
overexpression in Escherichia coli
pET overexpression system
-
single-copy gene in BAC clone T23L3
-