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2.7.1.52: fucokinase

This is an abbreviated version!
For detailed information about fucokinase, go to the full flat file.

Word Map on EC 2.7.1.52

Reaction

ATP
+
L-fucose
=
ADP
+
beta-L-fucose 1-phosphate

Synonyms

ATP:6-deoxy-beta-L-galactose 1-phosphotransferase, ATP:6-deoxy-L-galactose 1-phospho transferase, Fkgp, FKP, fucokinase, fucokinase/L-fucose-1-P-guanylyltransferase, fucokinase:GDP-fucose pyrophosphorylase, fucose kinase, Fuk, kinase, fuco- (phosphorylating), L-fucokinase, L-fucokinase/guanosine 5'-diphosphate-L-fucose pyrophosphorylase, L-fucokinase/guanosine-5'-diphosphate-Fuc pyrophosphorylase, L-fucokinase:GDP-fucose pyrophosphorylase, L-fucose kinase, L-fucose kinase/GDP-L-fucose pyrophosphorylase, More

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.52 fucokinase

Storage Stability

Storage Stability on EC 2.7.1.52 - fucokinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-16°C, about 30% loss of activity within 24 h and about 60% loss of activity within 72 h
-
-16°C, in the presence of stabilizing ovalbumin, about 30% loss of activity within 48 h
-
-20°C, 50% glycerol, 1 mM PMSF, 0.2 M NaCl, 50 mM Tris-HCl buffer, pH 7.5, stable for 1 month
-
-20°C, in the presence of 2-mercaptoethanol and glycerol, quite stable
-
-80°C, crude extracts, at least 2 years, without significant loss of activity
2-4°C, most stable in the presence of glycerol and a sulfhydryl reagent
-
4°C, 1 day, loss of 50% of GDP-L-fucose diphosphorylase activity
-
4°C, unstable in saline solution
-
frozen, in the presence of glycerol or sucrose and a sulfhydryl reagent, up to 1 week
-
frozen, rapid loss of activity, glycerol or sucrose and DTT or 2-mercaptoethanol stabilize, no stabilization by Mg2+, EDTA, KCl or ovalbumin
-
frozen, unstable in saline solution
-