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2.7.1.68: 1-phosphatidylinositol-4-phosphate 5-kinase

This is an abbreviated version!
For detailed information about 1-phosphatidylinositol-4-phosphate 5-kinase, go to the full flat file.

Word Map on EC 2.7.1.68

Reaction

ATP
+
1-phosphatidyl-1D-myo-inositol 4-phosphate
=
ADP
 +
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate

Synonyms

Diphosphoinositide kinase, More, Mss4, MSS4p, phosphatidylinositol 4,5-biphosphate kinase, phosphatidylinositol 4-phosphate 5-kinase, phosphatidylinositol 4-phosphate 5-kinase 2, phosphatidylinositol 4-phosphate 5-kinase alpha, phosphatidylinositol 4-phosphate 5-kinase beta, phosphatidylinositol 4-phosphate 5-kinase gamma, phosphatidylinositol 4-phosphate 5-kinase gamma661, phosphatidylinositol 4-phosphate 5-kinase Igamma, phosphatidylinositol 4-phosphate 5-kinase Igamma b, phosphatidylinositol 4-phosphate 5-kinase Igamma_i2, phosphatidylinositol 4-phosphate 5-kinase Igamma_v3, phosphatidylinositol 4-phosphate 5-kinase Igamma_v6, phosphatidylinositol 4-phosphate 5-kinase-Ibeta, phosphatidylinositol 4-phosphate kinase, phosphatidylinositol 5-kinase gamma, phosphatidylinositol kinase type I, phosphatidylinositol monophosphate 5-kinase, phosphatidylinositol phosphate 5-kinase, phosphatidylinositol phosphate 5-kinase 1, phosphatidylinositol phosphate 5-kinase 10, phosphatidylinositol phosphate 5-kinase Ibeta, phosphatidylinositol phosphate 5-kinase Igammai2, phosphatidylinositol phosphate kinase type I gamma, phosphatidylinositol-4-phosphate 5' kinase, phosphatidylinositol-4-phosphate 5-kinase, phosphatidylinositol-4-phosphate 5-kinase gamma 635, phosphatidylinositol-4-phosphate 5-kinase1alpha, phosphatidylinositol4-phosphate 5-kinase, phosphoinositol-4-phosphate-5-kinase, PI 4-phosphate 5-kinase, PI(4)P 5-kinase, PI(4)P5-K, PI4P 5-kinase, PI4P5K, PIP kinase, PIP-5kin, PIP5K, PIP5K type I, PIP5K type Ialpha, PIP5K-Ib, PIP5K1, PIP5K1A, PIP5K1alpha, PIP5K1gamma, PIP5K2, PIP5K3, PIP5K9, PIP5Kalpha, PIP5Kbeta, PIP5Kgamma, PIP5Kgamma635, PIP5Kgamma661, PIP5KI, PIP5KI gamma, PIP5KIalpha, PIP5KIbeta, PIP5KIgamma, PIP5KIgamma_i2, PIP5KIgamma_v3, PIP5KIgamma_v6, PIP5Ks, PIPK, PIPK1, PIPK10, PIPK1gamma-87, PIPKalpha, PIPKI, PIPKIalpha, PIPKIgamma, PIPKIgammaI2, PIPKIgammai5, PIPkin, PIPkin Igammab, PIPkinIgamma93, ppk-1, PtdIns(4)P 5-kinase, PtdIns(4)P kinase, PtdIns4P5K, PtdInsP, type 1 phosphatidylinositol-4-phosphate 5-kinase, type I gamma phosphatidylinositol phosphate 5-kinase i5, type I phosphatidylinositol 4-phosphate 5-kinase, type I phosphatidylinositol 4-phosphate 5-kinase gamma, type I phosphatidylinositol phosphate 5-kinase, type I phosphatidylinositol-4-phosphate 5-kinase, type I phosphatidylinositol-4-phosphate 5-kinase alpha, type I phosphatidylinositol-4-phosphate 5-kinase gamma, type I PI 4-phosphate 5-kinase, type I PtdInsP kinase, type Igamma phosphatidylinositol 4-phosphate 5-kinase, type Igamma PIP 5-kinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.68 1-phosphatidylinositol-4-phosphate 5-kinase

Cloned

Cloned on EC 2.7.1.68 - 1-phosphatidylinositol-4-phosphate 5-kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a yellow fluorescent (YFP) und GUS fusion PIP5K3 protein is expressed in Escherichia coli cells and transformed in Arabidopsis thaliana to produce trangenic plants. Overexpression and mutant is investigated.
cloning of splice variant phosphatidylinositol 4-phosphate 5-kinase Igamma_v6 from hippocampus, expression in COS-7 cells
-
cloning of splice variant phosphatidylinositol 4-phosphate 5-kinase Igamma_v6, expression in COS-7 cells
-
cloning of splice variant phosphatidylinositol 4-phosphate 5-kinase Igamma_v6, expression in COS-7 cells. In humans there is an alternative splice site 78 residues into exon 16c that splices out the rest of the Igamma_v5 insert, and then jumps either 1. to the start of exon 17 to generate human Igamma_v3 (the stop codon is then at the start of human exon 18), or 2. straight to that stop codon in exon 18 to generate human Igamma_v6, cloning of splice variants phosphatidylinositol 4-phosphate 5-kinase Igamma_v3, and Igamma_i2 and expression analysis
-
coexpression of wild-type HA-tagged isozyme alpha or HA-tagged isozyme alpha mutant D227A with HA-tagged Arf6 or HA-tagged Arf6 mutant N121I in COS-1 cells
-
EmPIP5K gene, DNA and amino acid sequence determination and analysis, located at position 710-8036 nt on the complimentary strand, structural organization of the PIP5K gene, and phylogenetic analysis and tree
enzyme form PIP5KIalpha
-
expressed as His6-tagged protein in Escherichia coli BL21(DE3) cells
-
expressed in Chinese hamster ovary cells
-
expressed in CHO cells
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL-21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli strain HT115 (DE3)
-
expressed in Escherichia coli strain Rosetta2 cells and Allium cepa epidermis cells
-
expressed in HEK-293T cells
-
expressed in HeLa cells
-
expressed in Nicotiana tabacum
expressed in Sf9 cells and MEF cells
-
expression in COS cells
-
expression in COS-7 cells
-
expression in Escherichia coli
-
expression in HEK-203 cell
-
expression in Xenopus laevis oocyte
-
expression of enzyme form 1 as GST-fusion protein in Escherichia coli, expression of His-tagged enzyme form 1 in Spodoptera frugiperda Sf9 cells via baculovirus infection system
expression of enzyme form 10 as GST-fusion protein in Escherichia coli, expression of His-tagged enzyme form 10 in Spodoptera frugiperda Sf9 cells via baculovirus infection system
expression of HA-tagged PIP5K isozymes in COS-7 cells, expression of isozyme PIP5Kalpha mutant D322A
-
expression of isozyme alpha as GST-fusion protein in Escherichia coli
expression of isozyme PIP5Kalpha tagged with monomeric red fluorescent protein, haemagglutinin, or FLAG in HEK-293T cells
expression of mutant enzymes K181A, D309N, D309N/R429Q and R427Q in Escherichia coli, expression of mutant enzymes in human MG-63 osteosarcoma cells
-
functional complementation of a yeast Mss4-1 mutant strain YOC808 by the wild-type isozyme gamma and the deletion mutants localizing to the plasma membrane, while the deletion mutants localized to the cytosol cannot support yeast cell growth, expression of wild-type and mutant type I HA-tagged or EGFP-tagged isozymes gamma in Neuro 2A cells and in COS-1 cells
-
functional expression in HeLa cells as GFP-fusion protein, translocation to the plasma membrane by coexpression of ARF6 upon stimulation by EGF, coexpression of ARF6 mutant N122I inhibits the membrane ruffling, overview
-
identification of splicing form c of type I phosphatidylinositol 4-phosphate 5-kinase isoform gamma possessing additional 26 amino acids at the C-terminus from a hippocampus library, DNA and amino acid sequence determination and analysis, overvexpression of the catalytically inactive mutants D316K and K188A of isozyme gamma splicing form c in rat cerebellar granule cells, expression of isozyme gamma splicing form a, expression of wild-type and FLAG-tagged isozyme gamma splicing form c in COS-7 cells
-
isozyme PIP5K1beta expression in HEK-293T and COS-7 cells
-
overexpression of isozymes alpha, beta, and gamma in HeLa cells, overexpression of isozymes alpha, beta, and gamma in CV-1 cells: isozymes alpha and beta are active, the latter shows the highest activity, isozyme gamma is not active, small interference RNA inhibition of isozyme beta expression also reduces expression of isozyme alpha, but increased expression of isozyme gamma, with a 50% loss in activity, reduction of isozyme alpha expression increases expression of isozymes beta and gamma and leaves the 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate synthesis level unaltered, inhibition of isozyme gamma increases expression of isozymes alpha and beta, overview, expression of isozyme beta increases the internalization of influenza virus hemagglutinin Y543, enzyme expression prevents the cells of disruption of their actine cytoskeleton by drugs, e.g. cytochalasin D or latrunculin, and increases the number of clathrin-coated pits
-
overexpression of mutant D266A in N1E-115 cells, expression of isozyme alpha in COS-7 cells, expression of isozyme alpha in Escherichia coli
-
overexpression of PIPKalpha in N1E-115 neuroblastoma cells, co-expression of His6-tagged KIF2A and GST-tagged PIPKalpha in Escherichia coli, binding analysis
-