2.7.1.71: shikimate kinase

This is an abbreviated version!
For detailed information about shikimate kinase, go to the full flat file.

Reaction

Synonyms

adenosine triphosphate: shikimate-3-phosphotransferase, AroK, AroL, AtSK1, AtSK2, kinase (phosphorylating), shikimate, kinase, shikimate (phosphorylating), MtSK, OsSK1, OsSK2, OsSK3, Rv2539c, shikimate kinase II, shikimate kinase-like 1, SK I, SK II, SK1, SK2, SKI, SKII, SKL1, type I shikimate kinase, aroK-encoded, type II shikimate kinase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.71 shikimate kinase

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Results	in table
45743	AA Sequence
7	Application
1	CAS Registry Number
19	Cloned(Commentary)
16	Cofactor
20	Crystallization (Commentary)
1	Expression
22	General Information
5	General Stability
14	IC50 Value
185	Inhibitors
8	kcat/KM [mM/s]
88	Ki Value [mM]
44	KM Value [mM]
1	Localization
51	Metals/Ions
28	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
731	Organism
6	Pathway
70	PDB ID
6	pH Optimum
2	pH Range
3	pI Value
1	Posttranslational Modification
33	Protein Variants
20	Purification (Commentary)
3	Reaction
1	Reaction Type
99	Reference
2	Renatured (Commentary)
10	Source Tissue
6	Specific Activity [micromol/min/mg]
5	Storage Stability
101	Substrates and Products (Substrate)
20	Subunits
51	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Range [°C]
5	Temperature Stability [°C]
15	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.7.1.71 - shikimate kinase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-18°C, 30% v/v glycerol, stable for several months with gradual loss of activity

Sorghum bicolor

-

641902

-20°C, 50% glycerol, aggregate containing 5 activities: EC 1.1.1.25, EC 2.7.1.71, EC 4.2.1.10, 5-dehydroquinate synthase and 3-enoyl-pyruvylshikimate 5-phosphate synthase is stable for at least 1 month

Neurospora crassa

-

137638

-20°C, in presence of 15% glycerol, stable for up to 4 weeks without loss of activity

Spinacia oleracea

-

641904

-20°C, protein concentration 1 mg/ml in 0.05% M Tris-HCl buffer, pH 7.5, 10 mM MgCl2, 0.1 M NaCl, 1 mM DTT, 10% loss of activity after 1 week, 90% loss of activity after 2 months

Escherichia coli

-

641903

4°C, 50% glycerol, aggregate containing 5 activities: EC 1.1.1.25, EC 2.7.1.71, EC 4.2.1.10, 5-dehydroquinate synthase and 3-enoyl-pyruvylshikimate 5-phosphate synthase is stable for at least 19 days

Neurospora crassa

-

137638