2.7.1.74: deoxycytidine kinase
This is an abbreviated version!
For detailed information about deoxycytidine kinase, go to the full flat file.
Word Map on EC 2.7.1.74
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2.7.1.74
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leukemia
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gemcitabine
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thymidine
-
triphosphate
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deaminase
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purine
-
ribonucleotide
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deoxynucleoside
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pyrimidine
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deoxyguanosine
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salvage
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myeloid
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arabinoside
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cytarabine
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prodrugs
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deoxyadenosine
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1-beta-d-arabinofuranosylcytosine
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deoxyribonucleoside
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5'-nucleotidase
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cladribine
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cross-resistance
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ara-ctp
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2-chlorodeoxyadenosine
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2',2'-difluorodeoxycytidine
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fludarabine
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dttp
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antileukemic
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ccrf-cem
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dfdctp
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2-chloro-2'-deoxyadenosine
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gemcitabine-resistant
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dado
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deoxycytidylate
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kinase-deficient
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clofarabine
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2',3'-dideoxycytidine
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5'-phosphorylation
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arabinosyl
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deoxypyrimidine
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t-lymphoblastoid
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deoxycoformycin
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medicine
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2'-deoxyadenosine
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deoxythymidine
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l-nucleoside
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dideoxycytidine
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t-lymphoblasts
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2',3'-dideoxyinosine
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tetrahydrouridine
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arabinosylcytosine
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anabolites
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pharmacology
-
analysis
- 2.7.1.74
- leukemia
- gemcitabine
- thymidine
- triphosphate
- deaminase
- purine
- ribonucleotide
- deoxynucleoside
- pyrimidine
- deoxyguanosine
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salvage
- myeloid
- arabinoside
- cytarabine
-
prodrugs
- deoxyadenosine
- 1-beta-d-arabinofuranosylcytosine
- deoxyribonucleoside
- 5'-nucleotidase
- cladribine
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cross-resistance
- ara-ctp
- 2-chlorodeoxyadenosine
- 2',2'-difluorodeoxycytidine
- fludarabine
- dttp
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antileukemic
-
ccrf-cem
-
dfdctp
- 2-chloro-2'-deoxyadenosine
-
gemcitabine-resistant
-
dado
- deoxycytidylate
-
kinase-deficient
- clofarabine
- 2',3'-dideoxycytidine
-
5'-phosphorylation
-
arabinosyl
-
deoxypyrimidine
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t-lymphoblastoid
- deoxycoformycin
- medicine
- 2'-deoxyadenosine
- deoxythymidine
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l-nucleoside
- dideoxycytidine
- t-lymphoblasts
- 2',3'-dideoxyinosine
- tetrahydrouridine
- arabinosylcytosine
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anabolites
- pharmacology
- analysis
Reaction
Synonyms
2'-deoxycytidine kinase, ara-C kinase, arabinofuranosylcytosine kinase, dC kinase, dCK, deoxycytidine kinase, deoxycytidine-cytidine kinase, dNTP:deoxycytidine 5'-phosphotransferase, kinase, deoxycytidine (phosphorylating), NTP:deoxycytidine 5'-phosphotransferase
ECTree
2.7.1.74 deoxycytidine kinaseAdvanced search results
results (
results (KM Value
KM Value on EC 2.7.1.74 - deoxycytidine kinase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.00076
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
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pH 7.6, 37°C, recombinant enzyme
0.00654
1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine
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pH 7.6, 37°C, recombinant enzyme
0.00061
1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine
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pH 7.6, 37°C, recombinant enzyme
0.00102
1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine
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pH 7.6, 37°C, recombinant enzyme
0.0013
2,5-difluoro-4-[1-(2-deoxy-beta-L-ribofuranosyl)]-aniline
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pH 7.6, 37°C, recombinant enzyme
0.0282
cytarabine
-
wild type enzyme, pH and temperature not specified in the publication
0.003
(-)-beta-2',3'-dideoxy-3'-thiacytidine
wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5
0.008
(-)-beta-2',3'-dideoxy-3'-thiacytidine
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5
0.008
(-)-beta-2',3'-dideoxy-3'-thiacytidine
wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5
0.015
(-)-beta-2',3'-dideoxy-3'-thiacytidine
C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37°C, pH 7.5
0.003
1-beta-D-arabinofuranosylcytosine
residues 65-79 deletion mutant with UTP as cosubstrate
0.003
1-beta-D-arabinofuranosylcytosine
wild type enzyme with UTP as cosubstrate
0.007
1-beta-D-arabinofuranosylcytosine
wild type enzyme with ATP as cosubstrate
0.013
1-beta-D-arabinofuranosylcytosine
residues 65-79 deletion mutant with ATP as cosubstrate
0.0131
1-beta-D-arabinosylcytosine
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.0506
1-beta-D-arabinosylcytosine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.1365
1-beta-D-arabinosylcytosine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.053
2'-deoxyadenosine
residues 65-79 deletion mutant with UTP as cosubstrate
0.091
2'-deoxyadenosine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
0.1146
2'-deoxyadenosine
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.1623
2'-deoxyadenosine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.415
2'-deoxyadenosine
residues 65-79 deletion mutant with ATP as cosubstrate
1.04
2'-deoxyadenosine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.001
2'-deoxycytidine
less than 0.001 mM, residues 65-79 deletion mutant with UTP as cosubstrate
0.001
2'-deoxycytidine
less than 0.001 mM, wild type enzyme with ATP as cosubstrate
0.001
2'-deoxycytidine
less than 0.001 mM, wild type enzyme with UTP as cosubstrate
0.0014
2'-deoxycytidine
residues 65-79 deletion mutant with ATP as cosubstrate
0.003
2'-deoxycytidine
Km below 0.003 mM, wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.0057
2'-deoxycytidine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.008
2'-deoxycytidine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.079
2'-deoxyguanosine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
0.087
2'-deoxyguanosine
residues 65-79 deletion mutant with UTP as cosubstrate
0.231
2'-deoxyguanosine
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.474
2'-deoxyguanosine
residues 65-79 deletion mutant with ATP as cosubstrate
1.865
2'-deoxyguanosine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
2.266
2'-deoxyguanosine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.0029
ATP
residues 65-79 deletion mutant with 2'-deoxycytidine as cosubstrate
0.0034
ATP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5
0.0084
ATP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
0.019
ATP
wild type enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6
0.025
ATP
C185A mutant enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6
0.053
ATP
residues 65-79 deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.0568
ATP
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
0.087
ATP
C185A mutant enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6
0.093
ATP
wild type enzyme with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.097
ATP
wild type enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6
0.0243
D-thymidine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.144
D-thymidine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.093
deoxyadenosine
at pH 8.0, temperature not specified in the publication
0.553
deoxyadenosine
at pH 8.0, temperature not specified in the publication
0.00034
deoxycytidine
at pH 8.0, temperature not specified in the publication
0.0007
deoxycytidine
at pH 8.0, temperature not specified in the publication
0.00542
deoxycytidine
-
wild type enzyme, pH and temperature not specified in the publication
0.122
deoxyguanosine
at pH 8.0, temperature not specified in the publication
0.527
deoxyguanosine
at pH 8.0, temperature not specified in the publication
12.457
deoxythymidine
at pH 8.0, temperature not specified in the publication
18.546
deoxythymidine
at pH 8.0, temperature not specified in the publication
7.502
deoxyuridine
at pH 8.0, temperature not specified in the publication
15.081
deoxyuridine
at pH 8.0, temperature not specified in the publication
0.0161
gemcitabine
wild type enzyme, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.0338
gemcitabine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.0467
gemcitabine
-
wild type enzyme, pH and temperature not specified in the publication
0.0562
gemcitabine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.0178
L-thymidine
mutant enzyme R104L/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.138
L-thymidine
mutant enzyme R104M/D133A, in 100 mM Tris, pH 7.5, 100 mM KCl, 10 mM MgCl2 and 1 mM ATP, at 37°C
0.011
troxacitabine
wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5
0.013
troxacitabine
wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5
0.057
troxacitabine
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5
0.058
troxacitabine
C9S/C45S/C59S/C146Smutant enzyme with UTP as phosphate donor, 37°C, pH 7.5
0.0015
UTP
residues 65-79 deletion mutant with 2'-deoxycytidine as cosubstrate
0.0023
UTP
wild type enzyme deletion mutant with 2'-deoxycytidine as cosubstrate
0.0047
UTP
residues 65-79 deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.008
UTP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5
0.01
UTP
wild type enzyme deletion mutant with 1-beta-D-arabinofuranosylcytosine as cosubstrate
0.011
UTP
wild type enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6
0.0149
UTP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
0.017
UTP
C185A mutant enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6
0.02
UTP
C185A mutant enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6
0.04
UTP
wild type enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6
0.0578
UTP
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
additional information
additional information
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Km-values of D-and L-analogues of cytidine and adenosine
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additional information
additional information
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linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate
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additional information
additional information
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linear kinetics with deoxyguanosine or cytidine, bimodal kinetics with deoxycytidine as substrate
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additional information
additional information
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Km-values of D-and L-cytidine analogues
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additional information
additional information
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Km-values of a variety of substrate derivatives
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additional information
additional information
no Michaelis-Menten kinetics, but negative cooperativity
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additional information
additional information
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steady-state fluorescent measurements, substrate binding constants, recombinant enzyme
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additional information
additional information
kinetic properties of the enzyme are modulated in vivo by phosphorylation of Ser74, mechanism, overview
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additional information
additional information
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kinetic properties of the enzyme are modulated in vivo by phosphorylation of Ser74, mechanism, overview
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