2.7.1.76: 2'-deoxyadenosine kinase
This is an abbreviated version!
For detailed information about 2'-deoxyadenosine kinase, go to the full flat file.
Word Map on EC 2.7.1.76
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2.7.1.76
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deaminase
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deoxynucleoside
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thymidine
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acidophilus
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5'-nucleotidase
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hypoxanthine-guanine
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ecto-5\'-nucleotidase
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9-beta-d-arabinofuranosyladenine
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mycoides
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deoxycoformycin
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2.7.1.20
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heterotropic
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medicine
- 2.7.1.76
- deaminase
- deoxynucleoside
- thymidine
- acidophilus
- 5'-nucleotidase
-
hypoxanthine-guanine
-
ecto-5\'-nucleotidase
- 9-beta-d-arabinofuranosyladenine
- mycoides
- deoxycoformycin
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2.7.1.20
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heterotropic
- medicine
Reaction
Synonyms
dAdo/dCyd kinase, dAK, dCK, dCyd kinase/dAdo kinase I, deoxyadenosine kinase, deoxycytidine kinase/deoxyadenosine kinase, dCK/dAK, deoxyguanosine kinase/deoxyadenosine kinase, dGK/dAK, dGuo kinase/dAdo kinase II, kinase, deoxyadenosine (phosphorylating), More, purine-deoxyribonucleoside kinase
ECTree
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KM Value
KM Value on EC 2.7.1.76 - 2'-deoxyadenosine kinase
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0.0335
gemcitabine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
0.016
ATP
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with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.021
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with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.01
deoxyadenosine
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with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.018
deoxyadenosine
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with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.0332
deoxyadenosine
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
0.027
in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS
0.121
deoxycytidine
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with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.146
deoxycytidine
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with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.019
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with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.02
deoxyguanosine
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with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.077
TTP
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with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
0.135
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with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
additional information
additional information
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the reaction with triphosphate follows strict Michaelis-Menten kinetics, while ATP exhibits negative cooperativity
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