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2.7.1.78: polynucleotide 5'-hydroxyl-kinase

This is an abbreviated version!
For detailed information about polynucleotide 5'-hydroxyl-kinase, go to the full flat file.

Word Map on EC 2.7.1.78

Reaction

ATP
+
5'-dephospho-DNA
=
ADP
 +
5'-phospho-DNA

Synonyms

5' end-specific RNA/DNA kinase, 5' polynucleotide kinase, 5'-hydroxyl polynucleotide kinase, 5'-hydroxyl polyribonucleotide kinase, 5'-hydroxyl RNA kinase, 5'-OH polynucleotide kinase, ATP:5'-dephosphopolynucleotide 5'-phosphatase, cleavage and polyadenylation factor I subunit, CLP1, Clp1-like protein, DNA 5'-hydroxyl kinase, DNA kinase, Grc3, HD-Pnk, HEAB, hPNKP, kinase (phosphorylating), polynucleotide 5'-hydroxyl, More, mPNKP, Nol9, PALF, PhoClp1, PNK, Pnk1, PNKP, polynucleotide 5'-hydroxyl kinase (phosphorylating), polynucleotide 5'-hydroxyl-kinase, polynucleotide kinase, polynucleotide kinase and aprataxin-like forkhead-associated, polynucleotide kinase phosphatase, polynucleotide kinase-phosphatase, polynucleotide kinase/phosphatase, RNA-specific polynucleotide kinase, Rnl1, RPNK, T4 PNK, T4 polynucleotide kinase, T4 polynucleotide kinase/phosphatase, tRNA endonuclease complex, TSEN

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.78 polynucleotide 5'-hydroxyl-kinase

Cloned

Cloned on EC 2.7.1.78 - polynucleotide 5'-hydroxyl-kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a vector encoding N-terminally Strep II epitope-tagged polynucleotide kinase is prepared, the protein is expressed in Escherichia coli Rosetta 2 DE3 pLysS cells
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)-RIL cells
expressed in Escherichia coli C41(DE3) cells
Tequatrovirus T4
-
expressed in Escherichia coli Rosetta2(DE3)pLysS cells
-
expression as soluble His-tagged protein in Escherichia coli
-
expression of GST-tagged human Nol9 in Spodoptera frugiperda Sf9 insect cells
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
expression of N-terminally His-tagged polynucleotide kinase and aprataxin-like forkhead-associated in Escherichia coli strain BL21(DE3)
-
expression of truncated enzyme, residue Met140 to C-terminus, in Escherichia coli
-
into the vector pET28-His10Smt3 for expression in Escherichia coli BL21DE3 cells
the PNKP mutants are subcloned into the vector pET16b for expression in Escherichia coli BL21DE3 pLysS cells