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2.7.1.78: polynucleotide 5'-hydroxyl-kinase

This is an abbreviated version!
For detailed information about polynucleotide 5'-hydroxyl-kinase, go to the full flat file.

Word Map on EC 2.7.1.78

Reaction

ATP
+
5'-dephospho-DNA
=
ADP
 +
5'-phospho-DNA

Synonyms

5' end-specific RNA/DNA kinase, 5' polynucleotide kinase, 5'-hydroxyl polynucleotide kinase, 5'-hydroxyl polyribonucleotide kinase, 5'-hydroxyl RNA kinase, 5'-OH polynucleotide kinase, ATP:5'-dephosphopolynucleotide 5'-phosphatase, cleavage and polyadenylation factor I subunit, CLP1, Clp1-like protein, DNA 5'-hydroxyl kinase, DNA kinase, Grc3, HD-Pnk, HEAB, hPNKP, kinase (phosphorylating), polynucleotide 5'-hydroxyl, More, mPNKP, Nol9, PALF, PhoClp1, PNK, Pnk1, PNKP, polynucleotide 5'-hydroxyl kinase (phosphorylating), polynucleotide 5'-hydroxyl-kinase, polynucleotide kinase, polynucleotide kinase and aprataxin-like forkhead-associated, polynucleotide kinase phosphatase, polynucleotide kinase-phosphatase, polynucleotide kinase/phosphatase, RNA-specific polynucleotide kinase, Rnl1, RPNK, T4 PNK, T4 polynucleotide kinase, T4 polynucleotide kinase/phosphatase, tRNA endonuclease complex, TSEN

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.78 polynucleotide 5'-hydroxyl-kinase

Inhibitors

Inhibitors on EC 2.7.1.78 - polynucleotide 5'-hydroxyl-kinase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
2-(hydroxy(3,4,5-trimethoxyphenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
-
A6B4C3
2-(hydroxy(phenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
-
A1B4C3
2-(hydroxy(thiophen-2-yl)methyl)-6-methyl-1-(phenylamino)-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione
-
A39B1C2
5'-AMP
-
weak
5'-Hydroxyl poly(I)
-
in combination with 5'-hydroxyl poly(A) or poly(C)
AgNO3
ammonium sulfate
beta,gamma-imidoadenosine 5'-triphosphate
-
binds with high affinity, similar to ATP
beta,gamma-imidoadenylyl 5'-tetraphosphate
Tequatrovirus T4
-
ATP analog, competitive against ATP, noncompetitive against 5'-OH-DNA, serves as substrate for the reverse reaction only
Ca2+
-
inhibits in combination with MgCl2, stimulates without MgCl2
Cd2+
-
-
chloramphenicol
Cibacron blue F3GA
Tequatrovirus T4
-
chromophore of blue dextran, inhibition is competitive to single stranded DNA, noncompetitive with respect to ATP
CTP
-
more than 90% inhibition at 0.3 mM
dATP
-
more than 95% inhibition at 0.3 mM
dCTP
-
80% inhibition at 0.3 mM
Deoxyribonucleoside triphosphates
-
-
Dextran sulfate
-
dGTP
-
more than 95% inhibition at 0.3 mM
diphosphate
disodium hydrogen phosphate
Tequatrovirus T4
-
-
dTTP
-
more than 95% inhibition at 0.3 mM
GTP
-
more than 90% inhibition at 0.3 mM
heparin
iodoacetate
N-ethylmaleimide
-
-
Na2HPO4
Tequatrovirus T4
-
-
Ni2+
-
inhibits in combination with MgCl2, stimulates without MgCl2
p-chloromercuribenzoate
p-hydroxymercuribenzoate
-
2-mercaptoethanol prevents inhibition
PEG 6000
-
5-15%, 3-4fold increase in activity, inhibitory above
phosphate
Ribonucleoside 3'-phosphates
-
weak
Ribonucleoside triphosphates
-
-
spermine
-
1 mM enhances activity 3times, inhibition above 1 mM
sulfate
sulfhydryl antagonists
-
-
-
tert-butyl 2-(1-hydroxy-2,2-diphenylethyl)-6-methyl-5,7-dioxo-2,4a,5,6,7,7a-hexahydro-1H-pyrrolo[3,4-b]pyridine-1yl-carbamate
-
A26B11C2
UTP
-
more than 90% inhibition at 0.3 mM
additional information
-