2.7.1.78: polynucleotide 5'-hydroxyl-kinase
This is an abbreviated version!
For detailed information about polynucleotide 5'-hydroxyl-kinase, go to the full flat file.
Word Map on EC 2.7.1.78
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2.7.1.78
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termini
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strand
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single-stranded
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gamma-32patp
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exonuclease
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32p-postlabeling
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5\'-hydroxyl
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bacteriophage
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phosphodiesterase
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3'-phosphatase
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nick
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32p-labeled
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duplex
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3'-monophosphate
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5'-phosphorylated
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5'-terminal
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postlabeling
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dsdna
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anticodon
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5\'-termini
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klenow
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xrcc4
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dinucleoside
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micrococcal
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3',5'-bisphosphate
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synthesis
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primase
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carcinogen-dna
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phosphotriester
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t4-infected
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oligoribonucleotide
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analysis
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biotechnology
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medicine
- 2.7.1.78
- termini
- strand
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single-stranded
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gamma-32patp
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exonuclease
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32p-postlabeling
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5\'-hydroxyl
- bacteriophage
- phosphodiesterase
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3'-phosphatase
- nick
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32p-labeled
- duplex
- 3'-monophosphate
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5'-phosphorylated
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5'-terminal
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postlabeling
- dsdna
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anticodon
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5\'-termini
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klenow
- xrcc4
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dinucleoside
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micrococcal
- 3',5'-bisphosphate
- synthesis
- primase
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carcinogen-dna
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phosphotriester
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t4-infected
- oligoribonucleotide
- analysis
- biotechnology
- medicine
Reaction
Synonyms
5' end-specific RNA/DNA kinase, 5' polynucleotide kinase, 5'-hydroxyl polynucleotide kinase, 5'-hydroxyl polyribonucleotide kinase, 5'-hydroxyl RNA kinase, 5'-OH polynucleotide kinase, ATP:5'-dephosphopolynucleotide 5'-phosphatase, cleavage and polyadenylation factor I subunit, CLP1, Clp1-like protein, DNA 5'-hydroxyl kinase, DNA kinase, Grc3, HD-Pnk, HEAB, hPNKP, kinase (phosphorylating), polynucleotide 5'-hydroxyl, More, mPNKP, Nol9, PALF, PhoClp1, PNK, Pnk1, PNKP, polynucleotide 5'-hydroxyl kinase (phosphorylating), polynucleotide 5'-hydroxyl-kinase, polynucleotide kinase, polynucleotide kinase and aprataxin-like forkhead-associated, polynucleotide kinase phosphatase, polynucleotide kinase-phosphatase, polynucleotide kinase/phosphatase, RNA-specific polynucleotide kinase, Rnl1, RPNK, T4 PNK, T4 polynucleotide kinase, T4 polynucleotide kinase/phosphatase, tRNA endonuclease complex, TSEN
ECTree
2.7.1.78 polynucleotide 5'-hydroxyl-kinaseAdvanced search results
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results (Natural Substrates Products
Natural Substrates Products on EC 2.7.1.78 - polynucleotide 5'-hydroxyl-kinase
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NATURAL SUBSTRATE 
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 5'-OH RNA
ADP + 5'-phospho-RNA
preference for RNA substrates
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r
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
the enzyme makes hydrogen bonds to the ribose 2'-hydroxyls of the 5'-terminal nucleoside, via Gln51, and the penultimate nucleoside, via Gln83
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-
?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Acetivibrio thermocellus DSM 1237
the enzyme makes hydrogen bonds to the ribose 2'-hydroxyls of the 5'-terminal nucleoside, via Gln51, and the penultimate nucleoside, via Gln83
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?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
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involved in repair of DNA single strand breaks
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?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
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?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
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involved in repair of DNA single strand breaks
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?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
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involved in repair of chromosomal DNA strand breaks that arise continously, but at low frequency, and are potentially lethal
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?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
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?
ATP + 5'-dephospho-DNA
ADP + 5'-phospho-DNA
Tequatrovirus T4
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-
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?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
Tequatrovirus T4
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involved in repair of broken RNA termini
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?
ATP + 5'-dephospho-RNA
ADP + 5'-phospho-RNA
Tequatrovirus T4
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involved in repair of broken RNA termini
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?
additional information
?
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Pnkp is a bifunctional enzyme: 2,3' cyclic phosphate and 5-OH ends are substrates for healing and sealing by Pnkp and Hen1, The 5' end is phosphorylated by the Pnkp kinase and the 2',3' cyclic phosphate is removed by the Pnkp phosphatase
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?
additional information
?
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Pnkp is a bifunctional enzyme: 2,3' cyclic phosphate and 5-OH ends are substrates for healing and sealing by Pnkp and Hen1, The 5' end is phosphorylated by the Pnkp kinase and the 2',3' cyclic phosphate is removed by the Pnkp phosphatase
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?
additional information
?
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PALF nuclease activity acts on single-stranded DNA or overhangs of duplex substrates. PALF does not open DNA hairpins. Reduction of PALF in vivo reduces the joining of incompatible DNA ends, PALF can function in concert with other nonhomologous DNA end joining proteins. PALF is able to resect 3 overhanging nucleotides and permit XRCC4-DNA ligase IV to complete the joining process in a manner that is as efficient as Artemis. Role for polynucleotide kinase and aprataxin-like forkhead-associated, PALF, in nonhomologous DNA end joining
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?
additional information
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PNKP function is modulated by interaction with the DNA repair scaffold proteins XRCC1 and XRCC4, which is mediated by binding of the PNKP forkhead-associated domain to phosphorylated motifs on XRCC1 and XRCC4, overview. Phosphorylation-independent interaction between PNKP and XRCC1 in human cells, since a non-phosphorylated XRCC1S518A/T519A/T523A triple mutant is also bound
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?
additional information
?
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the enzyme plays a role in tRNA splicing. The ATP-binding and/or hydrolysis capacity of CLP1 is required to enhance pre-tRNA cleavage
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?
additional information
?
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enzyme interacts with XRCC1, a scaffold protein that helps recruit repair enzymes at sites of single-strand breakage
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?
additional information
?
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PNKP function is modulated by interaction with the DNA repair scaffold proteins XRCC1 and XRCC4, which is mediated by binding of the PNKP forkhead-associated domain to phosphorylated motifs on XRCC1 and XRCC4, overview
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?
additional information
?
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Tequatrovirus T4
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in vivo role is possibly in maintaining DNA or RNA in the 5'-phosphorylated 3'-hydroxylated state which is the substrate for many reactions such as ligation and packaging
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?
additional information
?
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Tequatrovirus T4
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the enzyme catalyzes both, the phosphorylation of 5-hydroxyl termini and the hydrolysis of 3-phosphomonoesters and 2,3-cyclic phosphodiesters of polynucleotides
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?
additional information
?
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Tequatrovirus T4
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the enzyme catalyzes the phosphorylation of the 5'-hydroxyl terminus and the dephosphorylation of the 3'-phosphate terminus of DNA
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?
additional information
?
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Tequatrovirus T4 K10
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the enzyme catalyzes both, the phosphorylation of 5-hydroxyl termini and the hydrolysis of 3-phosphomonoesters and 2,3-cyclic phosphodiesters of polynucleotides
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?
