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2.7.1.91: sphingosine kinase

This is an abbreviated version!
For detailed information about sphingosine kinase, go to the full flat file.

Word Map on EC 2.7.1.91

Reaction

ATP
+
a sphingoid base
=
ADP
 +
a sphingoid base 1-phosphate

Synonyms

dihydrosphingosine kinase, kinase, dihydrosphingosine (phosphorylating), kinase, sphingosine (phosphorylating), More, SGK, SK, SK-1, SK-2, SK1, SK2, sphinganine kinase, sphingoid base kinase, sphingosine kinase, sphingosine kinase 1, sphingosine kinase 2, sphingosine kinase type 1, sphingosine kinase type 2, sphingosine kinase-1, sphingosine kinase-2, SPHK, SPHK-1, SPHK1, SPHK1a, SPHK1b, SPHK2, SPK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.91 sphingosine kinase

Cloned

Cloned on EC 2.7.1.91 - sphingosine kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
3- to 5fold overexpression in HUVEC cell
-
cloning and characterization of an isoform of rat SK1 with an N-terminal extension (long-rSK1). When the long-rSK1 is transfected in COS-7 cells, SK activity is 53fold increased. Substrate specificity and dependency on divalent cations of long-rSK1 are similar to those of rSK1
DNA and amino acid sequence determination and analysis
expressed in C2C12 cells
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells and HEK-293T cells
expressed in HEK -293T cells and embryonal carcinoma cells
expressed in HEK-293 cells
expressed in HEK-293T cells
expressed in KK/Ay type 2 diabetic mice
expressed in NIH3T3 cells
expressed in Saccharomyces cerevisiae strain KYA1
expressed in the adenovirus shuttle vector pAdlox
expression in HEK-293 cell
expression in HEK-293 cell line
-
expression of FLAG-tagged isozyme in HEK293 cells
expression of FLAG-tagged isozyme in HEK293 cells, expression of His-tagged isozyme in Sf9 insect cells via baculovirus infection system
expression of FLAG-tagged isozyme SK1 splicing variant a in HEK293 cells
expression of isoform SphK1a in HEK-293 cell
-
gene Sk1, DNA and amino acid sequence determination and analysis, 2 splicing variants, location on the X chromosome, functional overexpression of Sk1 in the yeast strain JSK398
gene Sk2, DNA and amino acid sequence determination and analysis, a single intron, location on chromosome 3, functional overexpression of Sk2 in the yeast strain JSK398
gene sphK1, sequence comparison of human isozymes sphingosine kinases 1 and 2
gene sphK2, sequence comparison of human isozymes sphingosine kinases 1 and 2
isoform SphK1 is expressed in Saccharomyces cerevisiae strain CGY32
isozymes SPHK1 and SPHK2, DNA and amino acid sequence determination and analysis
-
isozymes SPHK1 and SPHK2, expression in HEK293 cells as EGFP-fusion proteins
isozymes SPHK1 and SPHK2, several splicing variants are differently active, expression in HEK293 cells as EGFP-fusion proteins
overexpression in C2C12 cell
-
overexpression of gene sgkA leads to increased resistance to the anticancer drug cisplatin and altered growth behaviour
-
overexpression of isoform Sk1 in HUVEC cell
-
overexpression of isozymes SPHK1 and SPHK2 in cytosol of HEK293 cells
-
overview
phylogenetic analysis
-
quantitative real-time reverse transcriptase-PCR expression analysis
recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in High Five cells using the baculovirus transfection method
transfection of A498 kidney adenocarcinoma cells
transient expression in murine C2C12 myoblasts in the cytosolic fraction, in Golgi-enriched and endosome/plasma membrane fraction of transfected cells, the over-expressed SphK1 is localized at plasma membrane, as well as at internal membranes, SphK1 partially colocalized with caveolin-1, known to be an integral plasma membrane protein, secretion of extracellularly produced sphingosine 1-phosphate
-
transient expression of HA-tagged isozymes SphK1 and SphK2 in CHO-K1 cells
-