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2.7.2.1: acetate kinase

This is an abbreviated version!
For detailed information about acetate kinase, go to the full flat file.

Word Map on EC 2.7.2.1

Reaction

ATP
+
acetate
=
ADP
 +
acetyl phosphate

Synonyms

acetate kinase (phosphorylating), acetic kinase, acetokinase, ACK, ackA, AckA1, AckA2, ACKase, AK, ATP-ecoAK, ATP-specific AK, EAK, EutP, EutQ, MM_0495, Sak, short chain fatty acid kinase, StAckA, urkinase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.1 acetate kinase

Storage Stability

Storage Stability on EC 2.7.2.1 - acetate kinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, buffer containing 0.25 M sucrose, 40% of initial enzyme activity is lost within 2 months
-
-196°C, stable for at least 4 months in liquid nitrogen
-
-20°C, almost no decrease in activity within 1 year of storage
-
-20°C, can be stored in 20 mM Tris-HCl, pH 8.0, 2.0 mM MgCl2, 0.32 M NaCl, supplemented with 10% glycerol, without significant loss of activity for several weeks
-
-20°C, can be stored in 50 mM Tris-HCl, pH 7.4, 0.3 M NaCl and 2 mM dithiothreitol for several months without loss of activity
-
-20°C, relatively stable to storage when frozen, remains active for at least 1 year
-
-24°C, can be stored for several months with only a negligible loss in activity
-
-45°C, buffer containing 0.25 M sucrose, seems to be more stable than at -18°C
-
0-4°C, can be stored for some days with only a negligible loss in activity
-
0°C, even mild chilling overnight disrupts the protein conformation
-
4°C, 100 mM KCl, 50 mM disodium succinate, 20 mM TEA, pH 8.3, stable for at least 4 weeks
-
frozen in liquid nitrogen, the enzyme is stable for at least months