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2.7.2.3: phosphoglycerate kinase

This is an abbreviated version!
For detailed information about phosphoglycerate kinase, go to the full flat file.

Word Map on EC 2.7.2.3

Reaction

ATP
+
3-phospho-D-glycerate
=
ADP
 +
3-phospho-D-glyceroyl phosphate

Synonyms

3-PGK, 3-phosphoglycerate 1-phosphotransferase, 3-phosphoglycerate kinase, 3-phosphoglycerate phosphokinase, 3-phosphoglyceric acid kinase, 3-phosphoglyceric acid phosphokinase, 3-phosphoglyceric kinase, AbPGK, ATP-3-phospho-D-glycerate-1-phosphotransferase, ATP:3-phospho-D-glycerate 1-phosphotransferase, ATP:D-3-phosphoglycerate 1-phosphotransferase, chl-PGK, glycerate 3-phosphate kinase, glycerophosphate kinase, HacPGK1, HacPGK2, HapPGK, hPGK, HsPGK, human 3-phosphoglycerate kinase, kinase (phosphorylating), phosphoglycerate, Mfer_0156, OsPGK2, P-glycerate kinase, PAS-PGK, PfPGK, PGK, PGK 1, PGK-1, PGK-2, pgk-B, PGK1, PGK2, Pgk3, Pgk5, PGKA, PGKase-1, PGKB, PGKC, pgkp1, pgkp2, phosphoglycerate kinase, phosphoglycerate kinase 1, phosphoglycerate kinase 2, phosphoglycerate kinase B, phosphoglycerate kinase-1, phosphoglyceric acid kinase, phosphoglyceric kinase, phosphoglycerokinase, PwPGK, SjPGK, SSO0527, testis-specific phosphoglycerate kinase 2, TRSC58_02767, X chromosome-linked phosphoglycerate kinase-1

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.2 Phosphotransferases with a carboxy group as acceptor
                2.7.2.3 phosphoglycerate kinase

Cloned

Cloned on EC 2.7.2.3 - phosphoglycerate kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
129S6/SvEvTac genomic DNA fragments inserted into vector pUCBM21
-
1872-bp Pgk1/EGFP DNA fragment excised by double digestion of MluI and DraIII, consisting of 519 bp of the Pgk1 promoter, 723 bp of the EGFP cDNA and 224 bp of the bovine growth hormone (BGH) poly-A tail from the vector pPgk1/EGFP. Microinjection into the pronucleus of in vitro-fertilized FVB/NCrl mouse eggs and re-implanted into pseudopregnant ICR females to generate transgenic mice. CHO-K1 cells transfected with pPgk1/EGFP
3'UTR of PGK2 subcloned into the three regions F1, F2 and F3
-
a translational fusion of PGK and green fluorescent protein is used to transform tobacco BY-2 cells resulting in green fluorescent protein locating to the cell nuclei
-
cloned from genetic library, DNA sequence determination and analysis, expression in Escherichia coli as glutathione-S-transferase-fusion protein, which is subsequently cleaved off with thrombin
DNA and amino acid sequence determination and analysis, expression in Escherichia coli JM 83
DNA and amino sequence analysis, phylogenetic analysis, sequence comparisons, expression in Escherichia coli strain JM109
DNA sequence determination and expression in Escherichia coli BL21(DE3)
-
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL strain
-
expressed in Escherichia coli C41(DE3) pLysS cells
expressed in Escherichia coli Rosetta cells
expressed in Saccharomyces cerevisiae
expression in Escherichia coli
expression in Escherichia coli at optimal growth temperatures (37°C) yields a product which shows a tight association with a 28000 Da protein and exhibits low thermal stability suggesting a misfolding of the protein. As proved by N-terminal amino acid sequence analysis, the 28000 Da protein represents a 226-amino acid C-terminal fragment of the 3-phosphoglycerate kinase. Mutagenesis experiments confirm the assumption that the fragment originates by an internal translation initiation
expression in Escherichia coli, the protein expressed in the mesophilic host is folded correctly. By comparing the 3-phosphoglycerate kinase sequences of the mesophilic and the two thermophilic Archaea, trends in thermoadaptation are confirmed that can be deduced from comparisons of glyceraldehyde-3-phosphate dehydrogenase sequences from the same organisms. With increasing temperature the average hydrophobicity and the portion of aromatic residues increases, whereas the chain flexibility as well as the content in chemically labile residues (Asn, Cys) decreases
expression in Escherichia coli. The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from Sulfolobus solfataricus overlap by 8-bp
expression in Escherichia coli. The protein which is expressed in the mesophilic host is folded correctly
expression of His-tagged isozyme PGK1 in Escherichia coli XL1-Blue
expression of slightly modified wild-type in Escherichia coli
expression of the glycosomal enzyme lacking the C-terminal glycosome-specific 20-residue signal sequence, in Escherichia coli
-
expression vector pKK223-3, transformed into Escherichia coli strain JM109
-
functional overexpression of isozyme PGK1 in paclitaxel-sensitive, human osteogenic sarcoma cell line U-2OS, inducing a multidrug resistant phenotype
-
gene OsPgk2a-P or Os02g07260, genotype Pokkali, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant ectopic expression of splicing variant OsPgk2a-P in transgenic tobacco by Agrobacterium-mediated transformation improves its salinity stress tolerance by higher chlorophyll retention and enhances proline accumulation, besides maintaining better ion homeostasis. Ectopically expressing OsPgk2a-P transgenic tobacco plants show a tall phenotype with more number of pods than wild-type plants. The number of stomata in wild-type and ectopically expressing OsPgk2a-P plants. Transgenic plants show significantly higher stomatal density than wild-type plants. Morphology of stomata is normal and no heteromorphic stomata are observed
gene pgk, DNA and amino acid sequence determination, overexpression as native and His-tagged wild-type enzyme in Escherichia coli BL21(DE3), the His-tag strongly alters the enzymes properties, e.g. thermostability, kinetic parameters like kcat and Km
gene pgk, phylogenetic analysis, recombinant expression as soluble N-terminally His-tagged enzyme in Escherichia coli. Gene pgk is overexpressed in L-arginine and L-ornithine production strains and the production increases by 8% and by 17.5%, respectively
gene pgk, sequence comparison, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene pgk-A, quantitative RT-PCR enzyme expression analysis, recombinant expression of N-terminally His-tagged wild-type and truncated mutant enzymes and of the 80 amino acids long peptide corresponding to the PGKA N-terminal insertion in Escherichia coli strain BL21(DE3)
gene pgk-B, quantitative RT-PCR enzyme expression analysis
gene pgk-C, quantitative RT-PCR enzyme expression analysis
gene PGK1, DNA sequence determination and analysis
gene pgk1, quantitative RT-PCR enzyme expression analysis
gene PGK1, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain BL21(DE3)
gene pgk1, sequence comparisons and phylogenetic tree, real-time quantitative reverse transcription PCR enzyme expression analysis, recombinant expression of the his-tagged enzyme in Escherichia coli strain BL21 in supernatant and inclusion bodies
gene pgk2, quantitative RT-PCR enzyme expression analysis
gene PGKA, expression in Escherichia coli deficient mutant as a fusion protein, complementation of the mutant phenotype
-
gene PGKB, investigation of the stability of PGKB transcript using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) in combination with transcription and trans-splicing inhibitors, sinefungin and actinomycin D
gene PGKC, investigation of the stability of PGKB transcript using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) in combination with transcription and trans-splicing inhibitors, sinefungin and actinomycin D
histidine-tagged PGK
-
inducible expression of cytosolic isozyme in bloodstream parasites, toxicity depends on the expression level of active enzyme
-
into plasmid vector pEF-IRES, overexpressed in MKN-45 cells
-
into vector pET27b and expressed in Escherichia coli BL21 (DE3)pLysS cells
-
into vector pET27b and expressed in Escherichia coli BL21(DE3)pLysS cells
into vector pTRV2, introduced into Agrobacterium tumefaciens strain C58C1
-
mutant proteins cloned into the pGEM-T-easy vector, further cloned into the pET28 expression vector, wild-type and mutants overexpressed as N-terminal histidine-tailed recombinant proteins in Escherichia coli BL21DE3pLysS cells
-
normal or cancer-associated fibroblasts infected with the PGK1 or CXCL12 expressing vector
-
overexpressed in the Escherichia coli BL21-CodonPlus (DE3)-RIL
-
overexpression in Escherichia coli
overexpression in Saccharomyces cerevisiae
-
overexpression of glycosomal and cytosolic isozymes in Escherichia coli BL21
-
overexpression of PGK using transient transfection of pcDNA5-PGK
-
partial DNA and amino acid sequence determination
-
pRS313/Kllsm4DELTA1 plasmid, carrying a truncated form of the KlLSM4 gene of Kluyveromyces lactis introduced into yeast strain MCY4. Overexpression of the glycolitic gene PGK1 in MCY4/313Kllsm4DELTA1
-
subcloned between the EcoRI and PstI sites of the expression vector pKK223-3, expression in Escherichia coli strain JM109
the gene encoding phosphoglycerate kinase is inserted into a yeast expression vector under the control of the galactose-inducible GAL1 yeast promoter. This vector is then transformed into a pgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants are obtained on galactose plates at 37°C, but not at 28°C. These transformants contain low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast, a mesophile, therefore enables complementation of the yeast pgk defect at 37°C but not at 28°C
-
transient expression of two mislocalized GFP-tagged PGK mutants NOTP-PGK and NLS-PGK in cytoplasm and nucleus, respectively