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1
alpha-(RP)-borano substituted ADP
-
-
0.008
alpha-(SP)-borano substituted ADP
-
-
0.23 - 50
creatine phosphate
6.7
glycocyamine
at pH 8.5 and 25°C
0.51 - 8.97
phosphocreatine
additional information
additional information
-
0.015
ADP
-
30°C
0.015
ADP
-
ADP in form of MgADP-
0.017
ADP
-
pH 7.4, dimeric form
0.017
ADP
-
ADP in form of MgADP-
0.03
ADP
-
pH 7.0, muscle-type cytosolic isozyme
0.04
ADP
-
pH 7.0, brain-type cytosolic isozyme
0.043
ADP
-
pH 7.4, octameric form
0.043
ADP
-
ADP in form of MgADP-
0.051 - 0.052
ADP
-
30°C, pH 7.4
0.051 - 0.052
ADP
-
ADP in form of MgADP-
0.13
ADP
-
pH 7.0, ubiquitous mitochondrial isozyme
0.15
ADP
-
pH 7.0, ubiquitous mitochondrial isozyme
0.15
ADP
-
ADP in form of MgADP-
0.22
ADP
-
acetylcholine receptor membrane-asscociated enzyme
0.22
ADP
-
ADP in form of MgADP-
0.54
ADP
-
soluble enzyme from muscle
0.54
ADP
-
ADP in form of MgADP-
1.2
ADP
-
ADP in form of MgADP-
0.042
ATP
-
pH 7.4, dimeric form
0.042
ATP
-
ATP in form of MgATP2-
0.056
ATP
-
ATP in form of MgATP2-
0.082
ATP
-
pH 7.4, octameric form
0.082
ATP
-
ATP in form of MgATP2-
0.11
ATP
-
25°C, pH 8.0, ubiquitous mitochondrial isoform
0.11
ATP
-
pH 8.0, ubiquitous mitochondrial isozyme
0.2
ATP
-
cytoplasmic isoform M1, 25°C
0.22
ATP
pH 9.0, 30°C, recombinant wild-type enzyme, with substrate creatine
0.22
ATP
ATP in form of MgATP2-
0.29
ATP
pH 9.0, 30°C, recombinant wild-type enzyme, with substrate cyclocreatine
0.29
ATP
ATP in form of MgATP2-
0.3
ATP
-
hybrid form consisting of muscle and brain creatine kinase isoforms, pH and temperature not specified in the publication
0.31
ATP
-
mitochondrial isoform, 25°C
0.34
ATP
-
isoform III isolated after expression in Escherichia coli, pH 8.0, 30°C
0.35
ATP
-
wild-type hBBCK, pH and temperature not specified in the publication
0.36
ATP
-
isoform II isolated after expression in Escherichia coli, pH 8.0, 30°C
0.36
ATP
25°C, pH not specified in the publication, mutant T304K
0.37
ATP
-
reduced form of creatine kinase
0.38
ATP
-
wild-type containing both oxidized and reduced form
0.4
ATP
-
wild-type and isoform I isolated after expression in Escherichia coli, pH 8.0, 30°C
0.42
ATP
25°C, pH not specified in the publication, mutant S329A
0.43
ATP
-
isoform IV isolated after expression in Escherichia coli, pH 8.0, 30°C
0.43
ATP
mutant C74A, 25°C
0.43
ATP
-
oxidized form of creatine kinase
0.44
ATP
-
wild-type hMMCK, pH and temperature not specified in the publication
0.45
ATP
25°C, pH not specified in the publication, mutant N146C
0.46
ATP
-
wild-type enzyme
0.46
ATP
mutant A76G, 25°C
0.47
ATP
mutant C74S, 25°C
0.49
ATP
25°C, pH not specified in the publication, wild-type
0.51
ATP
mutant V72A, 25°C
0.56
ATP
wild type enzyme, at pH 8.0 and 25°C
0.56
ATP
recombinant wild type enzyme, at 25°C, pH not specified in the publication
0.57
ATP
mutant V75A, 25°C
0.58
ATP
25°C, pH not specified in the publication, mutant A205S
0.58
ATP
25°C, pH not specified in the publication, mutant Q46E
0.62
ATP
pH 9.0, 30°C, recombinant wild-type enzyme, with substrate N-ethylglycocyamine
0.62
ATP
25°C, pH not specified in the publication, mutant H267A
0.62
ATP
-
25°C, pH not specified in the publication, wild-type
0.62
ATP
ATP in form of MgATP2-
0.65
ATP
-
cytoplasmic isoform B, 25°C
0.65
ATP
-
25°C, pH not specified in the publication, mutant S205A
0.66
ATP
mutant C74M, 25°C
0.68
ATP
-
25°C, pH 8.0, sarcomeric mitochondrial isoform
0.68
ATP
-
pH 8.0, ubiquitous mitochondrial isozyme
0.68
ATP
-
25°C, euthermic squirrel
0.68
ATP
mutant G73A, 25°C
0.7
ATP
-
pH 9.0, 30°C, recombinant wild-type enzyme
0.7
ATP
-
cytoplasmic isoform M1, 25°C
0.7
ATP
mutant C74L, 25°C
0.7
ATP
-
ATP in form of MgATP2-
0.73
ATP
-
fusion protein CK-AK
0.73
ATP
-
25°C, pH not specified in the publication, mutant A267H
0.73
ATP
-
ATP in form of MgATP2-
0.74
ATP
-
25°C, pH not specified in the publication, mutant K304T
0.74
ATP
-
25°C, pH not specified in the publication, mutant L36K
0.75
ATP
-
cytoplasmic isoform M, 25°C
0.79
ATP
-
25°C, myosin bound enzyme
0.8
ATP
-
cytoplasmic isoform M1, 25°C
0.8
ATP
-
cytoplasmic isoform M2, 25°C
0.8
ATP
in form of Mg-ATP, at pH 8.5 and 25°C
0.81
ATP
-
pH 9.0, brain-type cytosolic isozyme
0.82
ATP
25°C, pH not specified in the publication, mutant A189D
0.84
ATP
-
25°C, pH not specified in the publication, mutant D189A
0.85
ATP
25°C, pH not specified in the publication, mutant K36L
0.86
ATP
-
fusion protein AK-CK
0.89
ATP
-
pH 9.0, muscle-type cytosolic isozyme
0.9
ATP
-
mitochondrial isoform M2, 25°C
0.91
ATP
-
25°C, pH not specified in the publication, mutant C146N
0.92
ATP
25°C, pH not specified in the publication, mutant E185Q
1
ATP
-
cytoplasmic isoform B, 25°C
1
ATP
-
cytoplasmic isoform M2, 25°C
1.09
ATP
-
25°C, pH not specified in the publication, mutant E46Q
1.1
ATP
-
pH 9.0, 30°C, recombinant mutant R340K
1.1
ATP
-
ATP in form of MgATP2-
1.14
ATP
recombinant mutant enzyme D326E, at 25°C, pH not specified in the publication
1.14
ATP
mutant enzyme D326E, at pH 8.0 and 25°C
1.18
ATP
-
25°C, hibernating squirrel
1.24
ATP
-
25°C, pH not specified in the publication, mutant Q185E
1.35
ATP
-
mitochondrial isoform M2, 25°C
1.37
ATP
-
0.020 mM organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C
1.38
ATP
-
0.005 mM organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C
1.38
ATP
-
25°C, pH not specified in the publication, mutant A329S
1.4
ATP
recombinant mutant enzyme H66R, at 25°C, pH not specified in the publication
1.4
ATP
mutant enzyme H66R, at pH 8.0 and 25°C
1.6
ATP
-
pH 9.0, 30°C, recombinant mutant R291K
1.6
ATP
-
pH 9.0, 30°C, recombinant mutant R340A
1.6
ATP
-
ATP in form of MgATP2-
1.6
ATP
-
ATP in form of MgATP2-
1.7
ATP
-
ATP in form of MgATP2-
1.7
ATP
in form of Mg-ATP, at pH 8.5 and 25°C
1.75
ATP
-
no organotellurium, preincubation time 30 min, cytosolic fraction, pH 7.5, 37°C
1.94
ATP
-
0.005 mM organotellurium, preincubation time 30 min, mitochondrial fraction, pH 7.5, 37°C
2.03
ATP
-
0.020 mM organotellurium, preincubation time 30 min, cytosolic fraction, pH 7.5, 37°C
2.1
ATP
-
0.005 mM organotellurium, preincubation time 30 min, cytosolic fraction, pH 7.5, 37°C
2.1
ATP
-
no organotellurium, preincubation time 5 min, mitochondrial fraction, including reduced glutathione (GSH), pH 7.5, 37°C
2.2
ATP
-
mutant enzyme D54G
2.49
ATP
-
0.020 mM organotellurium, preincubation time 30 min, mitochondrial fraction, pH 7.5, 37°C
2.82
ATP
-
no organotellurium, preincubation time 5 min, cytosolic fraction, pH 7.5, 37°C
2.84
ATP
-
0.005 mM organotellurium, preincubation time 5 min, cytosolic fraction, pH 7.5, 37°C
3.15
ATP
-
no organotellurium, preincubation time 30 min, mitochondrial fraction, pH 7.5, 37°C
3.19
ATP
-
no organotellurium, preincubation time 5 min, cytosolic fraction, including reduced glutathione (GSH), pH 7.5, 37°C
3.55
ATP
-
no organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C
3.6
ATP
-
pH 9.0, 30°C, recombinant mutant R235K
3.6
ATP
-
ATP in form of MgATP2-
3.92
ATP
-
0.020 mM organotellurium, preincubation time 5 min, cytosolic fraction, pH 7.5, 37°C
3.98
ATP
recombinant mutant enzyme D326A, at 25°C, pH not specified in the publication
3.98
ATP
mutant enzyme D326A, at pH 8.0 and 25°C
4.98
ATP
recombinant mutant enzyme H66P, at 25°C, pH not specified in the publication
4.98
ATP
mutant enzyme H66P, at pH 8.0 and 25°C
5.3 - 6
ATP
-
0.005 mM organotellurium, preincubation time 5 min, mitochondrial fraction, including reduced glutathione (GSH), pH 7.5, 37°C
6.68
ATP
-
0.005 mM organotellurium, preincubation time 5 min, cytosolic fraction, including reduced glutathione (GSH), pH 7.5, 37°C
7.3
ATP
-
pH 9.0, 30°C, recombinant mutant R340Q
7.3
ATP
-
ATP in form of MgATP2-
8.96
ATP
recombinant mutant enzyme H66P/D326A, at 25°C, pH not specified in the publication
8.96
ATP
mutant enzyme H66P/D326A, at pH 8.0 and 25°C
9.38
ATP
-
mitochondrial isoform sMiCK
10.3
ATP
-
pH 9.0, 30°C, recombinant mutant R129A
10.3
ATP
-
ATP in form of MgATP2-
17.52
ATP
-
0.020 mM organotellurium, preincubation time 5 min, cytosolic fraction, including reduced glutathione (GSH), pH 7.5, 37°C
20
ATP
-
0.020 mM organotellurium, preincubation time 5 min, mitochondrial fraction, including reduced glutathione (GSH), pH 7.5, 37°C
0.35
Creatine
-
mitochondrial isoform, 25°C
0.69
Creatine
-
37°C, atypical ubiquitous mitochondrial enzyme
0.74
Creatine
-
37°C, typical ubiquitous mitochondrial enzyme
1.01
Creatine
-
25°C, pH 8.0, ubiquitous mitochondrial isoform
1.01
Creatine
-
pH 8.0, ubiquitous mitochondrial isozyme
1.2
Creatine
-
25°C, myosin bound enzyme
1.5
Creatine
-
cytoplasmic isoform B, 25°C
1.62
Creatine
-
25°C, euthermic squirrel
2
Creatine
at pH 8.5 and 25°C
2 - 3
Creatine
-
isoform I isolated after expression in Escherichia coli, pH 8.0, 30°C
2 - 3.4
Creatine
-
25°C, pH not specified in the publication, mutant A329S
2.06
Creatine
-
25°C, hibernating squirrel
2.5
Creatine
-
soluble enzyme from muscle
2.7
Creatine
-
cytoplasmic isoform B, 25°C
2.8
Creatine
mutant L110D
3.1
Creatine
mutant L115D
3.26
Creatine
-
enzyme from embryonic stem cell-derived cardiomyocytes, pH 6.5, 37°C
3.4
Creatine
-
pH 7.4, dimeric form
3.6
Creatine
mutant L121D
3.9
Creatine
-
wild-type hBBCK, pH and temperature not specified in the publication
4.3
Creatine
-
mitochondrial isoform sMiCK
4.7
Creatine
-
cytoplasmic isoform M, 25°C
4.9
Creatine
-
pH 9.0, brain-type cytosolic isozyme
4.9 - 5
Creatine
-
30°C, pH 7.4
5
Creatine
-
hybrid form consisting of muscle and brain creatine kinase isoforms, pH and temperature not specified in the publication
5.67
Creatine
-
enzyme from neonatal cardiomyocytes, pH 6.5, 37°C
5.9
Creatine
-
25°C, pH not specified in the publication, wild-type
6.2
Creatine
-
wild-type hMMCK, pH and temperature not specified in the publication
6.6
Creatine
-
25°C, pH not specified in the publication, mutant S205A
7.1
Creatine
-
25°C, pH not specified in the publication, mutant L36K
7.31
Creatine
-
25°C, pH 8.0, sarcomeric mitochondrial isoform
7.31
Creatine
-
pH 8.0, ubiquitous mitochondrial isozyme
8.1
Creatine
-
pH 7.4, octameric form
8.2
Creatine
-
pH 9.0, 30°C, recombinant mutant R340A
8.33
Creatine
-
reduced form of creatine kinase
8.39
Creatine
-
wild-type containing both oxidized and reduced form
8.48
Creatine
-
oxidized form of creatine kinase
8.7
Creatine
mutant C74A, 25°C
8.7
Creatine
-
25°C, pH not specified in the publication, mutant A267H
8.9
Creatine
mutant A76G, 25°C
8.9
Creatine
-
25°C, pH not specified in the publication, mutant K304T
9
Creatine
-
pH 9.0, 30°C, recombinant wild-type enzyme
9
Creatine
mutant C74S, 25°C
9.1
Creatine
wild-type, 25°C
9.15
Creatine
wild type enzyme, at pH 8.0 and 25°C
9.15
Creatine
recombinant wild type enzyme, at 25°C, pH not specified in the publication
9.5
Creatine
-
pH 9.0, muscle-type cytosolic isozyme
9.6
Creatine
-
wild-type enzyme
10.5
Creatine
25°C, pH not specified in the publication, mutant S329A
10.8
Creatine
-
cytoplasmic isoform M1, 25°C
11
Creatine
25°C, pH not specified in the publication, mutant N146C
11.1
Creatine
-
25°C, pH not specified in the publication, mutant Q185E
11.2
Creatine
-
cytoplasmic isoform M2, 25°C
12.9
Creatine
-
25°C, pH not specified in the publication, mutant C146N
13
Creatine
-
fusion protein CK-AK
13.8
Creatine
mutant V75A, 25°C
14
Creatine
-
fusion protein AK-CK
14.2
Creatine
25°C, pH not specified in the publication, wild-type
14.5
Creatine
-
cytoplasmic isoform M1, 25°C
14.8
Creatine
-
25°C, pH not specified in the publication, mutant E46Q
16.7
Creatine
25°C, pH not specified in the publication, mutant H267A
17.1
Creatine
25°C, pH not specified in the publication, mutant Q46E
17.8
Creatine
25°C, pH not specified in the publication, mutant T304K
18.3
Creatine
mutant C74M, 25°C
18.6
Creatine
mutant C74L, 25°C
19
Creatine
-
pH 9.0, 30°C, recombinant mutant R340K
19
Creatine
-
isoform II isolated after expression in Escherichia coli, pH 8.0, 30°C
19.28
Creatine
recombinant mutant enzyme D326E, at 25°C, pH not specified in the publication
19.28
Creatine
mutant enzyme D326E, at pH 8.0 and 25°C
20
Creatine
-
isoform III isolated after expression in Escherichia coli, pH 8.0, 30°C
20.2
Creatine
-
mitochondrial isoform M2, 25°C
20.6
Creatine
-
25°C, pH not specified in the publication, mutant D189A
20.8
Creatine
25°C, pH not specified in the publication, mutant A205S
21
Creatine
-
wild-type and isoform IV isolated after expression in Escherichia coli, pH 8.0, 30°C
21.4
Creatine
mutant V72A, 25°C
21.6
Creatine
25°C, pH not specified in the publication, mutant K36L
23.5
Creatine
-
cytoplasmic isoform M1, 25°C
24.11
Creatine
recombinant mutant enzyme H66R, at 25°C, pH not specified in the publication
24.11
Creatine
mutant enzyme H66R, at pH 8.0 and 25°C
25
Creatine
mutant G73A, 25°C
25.5
Creatine
-
mitochondrial isoform M2, 25°C
26.38
Creatine
-
pH 9.0, 20°C
26.4
Creatine
-
cytoplasmic isoform M2, 25°C
28.5
Creatine
25°C, pH not specified in the publication, mutant A189D
34
Creatine
-
mutant D54G
34
Creatine
-
mutant enzyme D54G
44.1
Creatine
25°C, pH not specified in the publication, mutant E185Q
72
Creatine
-
pH 9.0, 30°C, recombinant mutant R235K
76
Creatine
-
pH 9.0, 30°C, recombinant mutant R291K
79.88
Creatine
recombinant mutant enzyme D326A, at 25°C, pH not specified in the publication
79.88
Creatine
mutant enzyme D326A, at pH 8.0 and 25°C
87.12
Creatine
recombinant mutant enzyme H66P, at 25°C, pH not specified in the publication
87.12
Creatine
mutant enzyme H66P, at pH 8.0 and 25°C
156.44
Creatine
recombinant mutant enzyme H66P/D326A, at 25°C, pH not specified in the publication
156.44
Creatine
mutant enzyme H66P/D326A, at pH 8.0 and 25°C
163
Creatine
-
pH 9.0, 30°C, recombinant mutant R340Q
167
Creatine
-
pH 9.0, 30°C, recombinant mutant R129A
0.23
creatine phosphate
-
pH 7.4, dimeric form
0.31
creatine phosphate
-
30°C
0.4
creatine phosphate
-
-
0.4
creatine phosphate
-
pH 7.0, 25°C
0.49 - 0.5
creatine phosphate
-
30°C, pH 7.4
0.68
creatine phosphate
-
pH 7.4, octameric form
1.07
creatine phosphate
-
37°C, ubiquitous isoform
1.19
creatine phosphate
-
37°C, sarcomeric isoform
1.9 - 2.2
creatine phosphate
-
-
1.9 - 2.2
creatine phosphate
-
acetylcholine receptor membrane-associated enzyme
2 - 10.6
creatine phosphate
-
-
2 - 10.6
creatine phosphate
-
-
2 - 10.6
creatine phosphate
-
-
3.7
creatine phosphate
-
30°C
17
creatine phosphate
-
0.5°C, pH 7.6
50
creatine phosphate
-
-
0.51
phosphocreatine
-
pH 7.0, brain-type cytosolic isozyme
0.55
phosphocreatine
-
pH 7.0, ubiquitous mitochondrial isozyme
0.61
phosphocreatine
-
0.005 mM organotellurium, preincubation time 5 min, mitochondrial fraction, including reduced glutathione (GSH), pH 7.5, 37°C
0.67
phosphocreatine
-
no organotellurium, preincubation time 5 min, mitochondrial fraction, including reduced glutathione (GSH), pH 7.5, 37°C
0.7
phosphocreatine
-
0.020 mM organotellurium, preincubation time 5 min, mitochondrial fraction, including reduced glutathione (GSH), pH 7.5, 37°C
0.8
phosphocreatine
-
pH 7.5, 37°C
0.87
phosphocreatine
-
no organotellurium, preincubation time 5 min, cytosolic fraction, pH 7.5, 37°C
1.11
phosphocreatine
-
no organotellurium, preincubation time 5 min, cytosolic fraction, including reduced glutathione (GSH), pH 7.5, 37°C
1.15
phosphocreatine
-
0.005 mM organotellurium, preincubation time 5 min, cytosolic fraction, including reduced glutathione (GSH), pH 7.5, 37°C
1.16
phosphocreatine
-
pH 7.0, ubiquitous mitochondrial isozyme
1.32
phosphocreatine
-
0.005 mM organotellurium, preincubation time 5 min, cytosolic fraction, pH 7.5, 37°C
1.33
phosphocreatine
-
pH 7.0, muscle-type cytosolic isozyme
1.34
phosphocreatine
-
no organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C
1.41
phosphocreatine
-
0.020 mM organotellurium, preincubation time 5 min, cytosolic fraction, pH 7.5, 37°C
1.72
phosphocreatine
-
0.020 mM organotellurium, preincubation time 5 min, cytosolic fraction, including reduced glutathione (GSH), pH 7.5, 37°C
1.73
phosphocreatine
-
0.005 mM organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C
2.04
phosphocreatine
-
0.020 mM organotellurium, preincubation time 5 min, mitochondrial fraction, pH 7.5, 37°C
3.26
phosphocreatine
-
0.005 mM organotellurium, preincubation time 30 min, cytosolic fraction, pH 7.5, 37°C
3.34
phosphocreatine
-
0.020 mM organotellurium, preincubation time 30 min, cytosolic fraction, pH 7.5, 37°C
3.54
phosphocreatine
-
no organotellurium, preincubation time 30 min, cytosolic fraction, pH 7.5, 37°C
5.77
phosphocreatine
-
0.005 mM organotellurium, preincubation time 30 min, mitochondrial fraction, pH 7.5, 37°C
6.43
phosphocreatine
-
0.020 mM organotellurium, preincubation time 30 min, mitochondrial fraction, pH 7.5, 37°C
8.97
phosphocreatine
-
no organotellurium, preincubation time 30 min, mitochondrial fraction, pH 7.5, 37°C
additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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kinetics
-
additional information
additional information
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kinetics
-
additional information
additional information
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kinetics
-
additional information
additional information
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kinetics
-
additional information
additional information
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
overview
-
additional information
additional information
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overview
-
additional information
additional information
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overview
-
additional information
additional information
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overview
-
additional information
additional information
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overview
-
additional information
additional information
-
overview
-
additional information
additional information
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overview
-
additional information
additional information
-
overview
-
additional information
additional information
-
effect of temperature on values for MgATP2- and creatine
-
additional information
additional information
-
dextran strongly increases Km
-
additional information
additional information
-
temperature dependence of reaction, in vivo measurements
-
additional information
additional information
-
kinetics of wild-type and mutant enzymes
-
additional information
additional information
-
kinetic mechanism, the enzyme shows negative cooperativity and nonidentical active sites
-
additional information
additional information
-
kinetic mechanism, the enzyme shows negative cooperativity and nonidentical active sites
-
additional information
additional information
-
kinetics for ADP in mitochondria, enhancing effect of creatine, overview
-
additional information
additional information
-
kinetics for ADP in mitochondria, enhancing effect of creatine, overview
-
additional information
additional information
-
kinetics for ADP in mitochondria, enhancing effect of creatine, overview
-
additional information
additional information
-
kinetics, negative cooperativity
-
additional information
additional information
kinetics, recombinant wild-type and mutant enzymes
-
additional information
additional information
-
kinetics, recombinant wild-type and mutant enzymes
-
additional information
additional information
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kinetics, the enzyme shows negative cooperativity and nonidentical active sites
-
additional information
additional information
-
kinetics, the enzyme shows negative cooperativity and nonidentical active sites
-
additional information
additional information
kinetics, the enzyme shows negative cooperativity and nonidentical active sites
-