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2.7.3.4: taurocyamine kinase

This is an abbreviated version!
For detailed information about taurocyamine kinase, go to the full flat file.

Word Map on EC 2.7.3.4

Reaction

ATP
+
Taurocyamine
=
ADP
 +
N-phosphotaurocyamine

Synonyms

ATP:taurocyamine phosphotransferase, CsTK, guanidino kinase, hypotaurocyamine kinase, kinase (phosphorylating), taurocyamine, kinase, taurocyamine (phosphorylating), MiTK, More, phosphagen kinase, PK1, PK2, SmGK, taurocyamine kinase, taurocyamine phosphotransferase, TK, TPK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.3 Phosphotransferases with a nitrogenous group as acceptor
                2.7.3.4 taurocyamine kinase

Cloned

Cloned on EC 2.7.3.4 - taurocyamine kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli TB1 cells
expression in Escherichia coli TB1
gene CLF_107872, DNA and amino acid sequence determination and analysis, sequence comarisons and phylogenetic analysis, the coding region of the cDNA of D1D2 is cloned into the pMAL-c2 vector and expressed as maltose binding protein fusion protein in Escherichia coli strain TB1, quantitative real-time PCR enzyme expression analysis. Recombinant expression of N-terminally GST-tagged enzyme D1 and D2 mutants in Escherichia coli strain BL21(DE3) pLysS
gene PHYSODRAFT_566100, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of N-terminal His6-PsPK protein in Escherichia coli strain Rosetta 2 (DE3)
recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
sequence comparisons, recombinant expression of enzyme domain1 and domain2 mutants in Escherichia coli strain TB1
the maltose binding protein-fused enzyme is expressed in Escherichia coli BL21(DE3) cells