2.7.3.4: taurocyamine kinase
This is an abbreviated version!
For detailed information about taurocyamine kinase, go to the full flat file.
Word Map on EC 2.7.3.4
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2.7.3.4
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creatine
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glycocyamine
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lombricine
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annelid
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polychaete
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cdna-derived
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arenicola
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2.7.3.3
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neanthes
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paragonimus
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phosphoarginine
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westermani
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drug development
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pharmacology
- 2.7.3.4
- creatine
- glycocyamine
- lombricine
-
annelid
-
polychaete
-
cdna-derived
- arenicola
-
2.7.3.3
- neanthes
- paragonimus
- phosphoarginine
- westermani
- drug development
- pharmacology
Reaction
Synonyms
ATP:taurocyamine phosphotransferase, CsTK, guanidino kinase, hypotaurocyamine kinase, kinase (phosphorylating), taurocyamine, kinase, taurocyamine (phosphorylating), MiTK, More, phosphagen kinase, PK1, PK2, SmGK, taurocyamine kinase, taurocyamine phosphotransferase, TK, TPK
ECTree
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Crystallization
Crystallization on EC 2.7.3.4 - taurocyamine kinase
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purified recombinant wild-type and mutant enzymes complexed with taurocyamine or L-arginine, sitting drop vapor diffusion method, mixing of 5 mg/ml protein in 20mM Tris-HCl, pH 8.5, with reservoir solution, containing 200mM diammonium tartrate, pH 5.4, 20% w/v PEG 3350, 20% v/v ethylene glycol, in a 2:1 or 1:1 protein/solution volume ratio, 2 weeks, 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution leads to a small angle x-ray scattering model of SmTK-TSA in solution with two closed active sites, molecular replacement. The SmTK crystal is soaked with the dead end transition state analogue components taurocyamine-NO3 2-MgADP
sitting-drop vapour-diffusion, 100 mM MES, pH 6.5, 25%(w/v) PEG 3000, 290 K, unit-cell parameters a = 52.7, b = 122.1, c = 63.2 A , beta = 108.5°, space group P21, 2.8 A resolution on ESRF beamline ID29