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2.7.4.1: ATP-polyphosphate phosphotransferase

This is an abbreviated version!
For detailed information about ATP-polyphosphate phosphotransferase, go to the full flat file.

Word Map on EC 2.7.4.1

Reaction

ATP
+
(Phosphate)n
=
ADP
 +
(phosphate)n+1

Synonyms

AAur2811, AjPPK, ATP:polyphosphate phosphotransferase, AUJ86_09545, CHU0107, CHU_0107, class I PPK2, class II polyphosphate kinase, class III PPK2, Deipr_1912, DR0132, DR_0132, ES2, family-2 polyphosphate kinase, FTT_1564, glycogen-bound polyphosphate kinase, GmSuSy, I601_4057, K649_10090, kinase, polyphosphate (phosphorylating), More, MrH2468, Mrub_2488, MXAN_0056, NCg12620, OsIPK2, PA0141, PA3455 protein, paPpx, plyphosphate kinase 1, poly P kinase 1, poly(P) kinase 1, poly(P) kinase 2, poly-P kinase, poly-P kinase 1, polyP kinase, polyphosphate kinase, polyphosphate kinase 1, polyphosphate kinase 2, polyphosphate kinase 3, polyphosphate kinase-1, polyphosphate kinase-2, polyphosphate-dependent nucleoside diphosphate kinase, polyphosphate: ADP phosphotransferase, polyphosphate:ADP phosphotransferase, polyphosphate:nucleotide phosphotransferase, polyphosphoric acid kinase, PPK, PPK-2, PPK1, PPK2, PPK2-1, PPK2-2, PPK2-3, PPK2a, PPK2B, PPK2c, PPK2d, PPK2e, PPK3, Rv3232c, SMc02148, SMc02148 protein, SPO0224, SPO1256, SPO1727

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.1 ATP-polyphosphate phosphotransferase

Cloned

Cloned on EC 2.7.4.1 - ATP-polyphosphate phosphotransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
complementation of the PPK1-deficient mutant strain PAOM5 by the wild-type PPK1
-
Escherichia coli strain harboring plasmid pSPK5 with ppk gene increases enzyme activity of polyphosphate kinase, resulting in increased accumulation of polyphosphate in Escherichia coli
expressed in Enterobacter aerogenes IAM1183
-
expressed in Enterobacter aerogenes strain IAM1183
-
expressed in Escherichia coli BL-21 (DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta2 (DE3) pLysS cells
-
expressed in Escherichia coli strain M15
-
expressed in Escherichia coli strain NR 100
-
expressed in Escherichia coli strains BL21 (DE3) and B834 (DE3)
expressed in Pseudomonas putida strain KT2440
-
expressed in Streptomyces lividans
-
expression in Escherichia coli
expression in Escherichia coli TOP10 cells
expression of His6-tagged Ppk in Escherichia coli strain BL21(DE3)
Thermosynechococcus vestitus
expression of polyphosphate kinase 2 gene in Escherichia coli
expression of type I polyphosphate kinase in Escherichia coli
expression with an N-terminal His-tag in Escherichia coli
gene Ddppk1, overexpression under control of the strong promoter Pact15, functional expression of GFP-DdPPK1 fusion protein
gene FTT1564, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 Rosetta pLysS (DE3)
gene ppk, expression analysis
gene ppk, expression analysis, quantitative real-time PCR assay for polyphosphate kinase genes in activated sludge, method development and optimization, overview
Q9S646
gene ppk, quantitative expression analysis in wild-type and transfected recombinant cells, overview, expression in HEK-293 and HEK-293T cells, HeLa cells, Cos-7 cells
gene ppk, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene ppk, subcloning in Escherichia coli strain TOP10
gene ppk1, DNA and amino acid sequence determination and analysis, expression analysis in several different sludges, phylogenetic analysis
gene ppk1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH10B, expression analysis, quantitative real-time PCR assay for polyphosphate kinase genes in activated sludge, method development and optimization, overview
gene ppk1, DNA and amino acid sequence determination and analysis, from at least five clades of Candidatus Accumulibacter, phylogenetic analysis
gene ppk1, expression of wild-type and mutant enzymes
gene ppk1, the ppk1 promoter of Mycobacterium tuberculosis is activated under phosphate starvation, involvement of SigE in ppk1 transcription, binding of SigE to the ppk1 promoter. Quantitative RT-PCR ppk1 expression analysis. Gene ppk1 in vitro transcription
gene ppx, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strains XL10-Gold and BL21-CodonPlus
-
gene SPO0224, DNA and amino acid sequence determination and analysis, recombinant expression of Strep-tagged isozyme PPK2-3 in Escherichia coli strain BL21(DE3)-T1
gene SPO1256, DNA and amino acid sequence determination and analysis, recombinant expression of STrep-tagged isoyzme PPK2-2 in Escherichia coli strain BL21(DE3)-T1
gene SPO1727, DNA and amino acid sequence determination and analysis, recombinant expression of Strep-tagged isozyme PPK2-1 in Escherichia coli strain BL21(DE3)-T1
genes NCgl0880 and NCgl2620 or pkk2A and pkk2B, expression of wild-type and mutant PKK2B isozymes in Escherichia coli strain Bl21(DE3), subcloning in Escherichia coli strain DH5alpha
-
His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells
-
overexpression in Escherichia coli
ppk1, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain
ppk1b, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain
ppk2a, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain
ppk2b, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain
ppk2c, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain
ppk2d, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain
ppk2e, DNA and amino acid sequence determination and analysis, recombinant expression of eYFP-tagged protein in Escherichia coli DELTAppk strain