2.7.4.10: nucleoside-triphosphate-adenylate kinase

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For detailed information about nucleoside-triphosphate-adenylate kinase, go to the full flat file.

Reaction

Synonyms

adenylate kinase 3, adenylate kinase 4, AK3, AK4, CsAK3, GAK, GTP: AMP phosphotra nsferase, GTP:AMP phosphotransferase, guanosine triphosphate-adenylate kinase, kinase, nucleoside triphosphate-adenylate (phosphorylating), nucleoside triphosphate-adenosine monophosphate transphosphorylase, nucleoside triphosphate-adenylate kinase, ssozyme 3 of adenylate kinase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.4 Phosphotransferases with a phosphate group as acceptor

                2.7.4.10 nucleoside-triphosphate-adenylate kinase

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Results	in table
2041	AA Sequence
1	Application
1	CAS Registry Number
7	Cloned(Commentary)
5	Crystallization (Commentary)
42	Disease/ Diagnostics
2	Expression
2	General Information
7	General Stability
17	Inhibitors
9	Ki Value [mM]
13	KM Value [mM]
9	Localization
11	Metals/Ions
7	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
16	Organism
2	Pathway
11	PDB ID
5	pH Optimum
4	pH Range
1	Protein Variants
6	Purification (Commentary)
2	Reaction
1	Reaction Type
15	Reference
1	Renatured (Commentary)
11	Source Tissue
5	Specific Activity [micromol/min/mg]
4	Storage Stability
41	Substrates and Products (Substrate)
2	Subunits
18	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Temperature Stability [°C]

Temperature Stability

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Temperature Stability on EC 2.7.4.10 - nucleoside-triphosphate-adenylate kinase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0

Sus scrofa

-

5 min, 2 mg protein/ml, stable in 0.1 N HCl

642538

30 - 65

Saccharomyces cerevisiae

-

soluble isoenzyme Adk2p (long), 66% of the maximum activity at 30°C, about 16% activity at 65°C

662361

35

Saccharomyces cerevisiae

-

soluble isoenzyme Adk2p (short) inactivated

662361

40

Sus scrofa

-

5 min, 2 mg protein/ml, in 0.1 N HCl, 70% loss of activity

642538

95

Sus scrofa

-

5 min, 95% inactivation at pH 7.4, 0.15 M imidazole buffer

642538

additional information

Bos taurus

-

35% ammonium sulfate, AMP or other substrates enhance thermal stability

642534