2.7.4.14: UMP/CMP kinase
This is an abbreviated version!
For detailed information about UMP/CMP kinase, go to the full flat file.
Word Map on EC 2.7.4.14
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2.7.4.14
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dcmp
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deoxycytidine
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gemcitabine
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cidofovir
-
nmp-binding
-
umpks
-
medicine
-
diagnostics
- 2.7.4.14
- dcmp
- deoxycytidine
- gemcitabine
- cidofovir
-
nmp-binding
- umpks
- medicine
- diagnostics
Reaction
Synonyms
ATP:UMP-CMP phosphotransferase, CMP kinase, CMP/UMP kinase, CMPK, CMPK1, CMPK2, CTP:CMP phosphotransferase, cytidine monophosphate kinase, cytidine/uridine monophosphate kinase 2, cytidylate kinase, dCK, deoxycytidine kinase, EC 2.7.4.5, kinase, cytidylate (phosphorylating), More, MssA protein, nucleoside monophosphate kinase, P25, pyrimidine nucleoside monophosphate kinase, UCK, UMP-CMP kinase, UMP-CMP kinase 2, UMP-CMPK2, UMP/CMP kinase, UMP/CMP kinase 1, UMP/CMPK1, UMPK, uridine monophosphate/cytidine monophosphate kinase
ECTree
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Substrates Products
Substrates Products on EC 2.7.4.14 - UMP/CMP kinase
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REACTION DIAGRAM
ATP + (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine
ADP + (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine
Cidofovir
-
-
?
ATP + 2'-azido-CMP
ADP + 2'-azido-CDP
-
4fold higher activity with 2'-azido-dCMP than with 2'-azido-CMP
-
-
?
ATP + 2'-azido-dCMP
ADP + 2'-azido-dCDP
-
4fold higher activity with 2'-azido-dCMP than with 2'-azido-CMP
-
-
?
ATP + 2'-azido-dUMP
ADP + 2'-azido-dUDP
-
similar activity with 2'-azido-UMP and 2'-azido-dUMP
-
-
?
ATP + 2'-azido-UMP
ADP + 2'-azido-UDP
-
similar activity with 2'-azido-UMP and 2'-azido-dUMP
-
-
?
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
ADP + beta-L-2',3'-dideoxy-3'-thiacytidine diphosphate
-
-
-
-
?
ATP + gemcitabine
ADP + gemcitabine monophosphate
activity of DCK
i.e. 2',2'-difluorodeoxycytidine 5'-monophosphate
-
?
ATP + gemcitabine phosphate
ADP + gemcitabine diphosphate
activity of CMPK
i.e. 2',2'-difluorodeoxycytidine 5'-diphosphate
-
?
ATP + 5-aza-cidofovir
ADP + phospho-5-aza-cidofovir
wild-type enzyme
-
-
?
ADP + phospho-cidofovir
i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid
-
-
?
ATP + cidofovir
ADP + phospho-cidofovir
i.e. (S)-[1-(4-amino-2-oxo-pyrimidin-1-yl)-3-hydroxy-propan-2-yl]oxy-methylphosphonic acid, wild-type enzyme
-
-
?
ATP + CMP
ADP + CDP
-
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
-
-
?
ATP + CMP
ADP + CDP
-
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and dCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
-
-
?
ADP + phospho-cytarabine
anticancer drug, phosphorylation catalyzed by the enzyme in cidofovir-resistant HaCaT cells
-
-
?
ATP + cytarabine
ADP + phospho-cytarabine
i.e. araC, wild-type enzyme and mutant P64T
-
-
?
ATP + dCMP
ADP + dCDP
-
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
-
-
?
ATP + dCMP
ADP + dCDP
-
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
-
-
?
ATP + dCMP
ADP + dCDP
-
higher activity with the D-enantiomer compared to the L-enantiomer
-
-
?
ATP + dCMP
ADP + dCDP
-
no evidence for reversal of the reaction
-
?, ir
ATP + dUMP
ADP + dUDP
-
higher activity with the D-enantiomer compared to the L-enantiomer
-
-
?
ADP + phospho-gemcitabine
i.e. 2',2'-difluorodeoxycytidine
-
-
?
ATP + gemcitabine
ADP + phospho-gemcitabine
i.e. 2',2'-difluorodeoxycytidine, wild-type enzyme
-
-
?
ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP
-
-
-
-
?
ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP
ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP
-
-
-
-
?
ATP + L-dCMP
ADP + L-dCDP
slower phosphorylation than the D-enantiomers
-
-
?
ATP + L-dUMP
ADP + L-dUDP
slower phosphorylation than the D-enantiomers
-
-
?
ATP + UMP
ADP + UDP
-
the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis
-
-
?
ATP + UMP
ADP + UDP
-
the enzyme catalyses an important step in the phosphorylation of UTP, CTP and cCTP. It is also involved in the necessary phosphorylation by cellular kinases of nucleoside analogs used in antiviral therapies
-
-
?
?
-
-
formation of a ternary complex, addition of substrates is random
-
-
?
additional information
?
-
-
the UMP-CMP kinase has a relaxed enantiospecificity for the nucleoside monophosphate acceptor site, but it is restricted to D-nucleotides at the donor site
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-
?
additional information
?
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the enzyme is involved in the phosphorylation of nucleic acid precursors, and is critical in the ribo- and deoxyribonucleoside salvage pathway, as well as the anabolic phosphorylation of nucleoside analogues used in viral and anticancer therapies, e.g. cidofovir, which is activated by the enzyme, overview
-
-
?
additional information
?
-
-
the enzyme is involved in the phosphorylation of nucleic acid precursors, and is critical in the ribo- and deoxyribonucleoside salvage pathway, as well as the anabolic phosphorylation of nucleoside analogues used in viral and anticancer therapies, e.g. cidofovir, which is activated by the enzyme, overview
-
-
?
additional information
?
-
UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response
-
-
?
additional information
?
-
-
UMP-CMPK2 may have other functions in addition to the supply of substrates for mtDNA synthesis, it is highly expressed in leukemia cells, in bone marrow, and is tightly correlated with macrophage activation and inflammatory response
-
-
?
additional information
?
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substrate/product binding site determination and kinetics using MABA-CDP in a competition assay, overview
-
-
?
additional information
?
-
-
substrate/product binding site determination and kinetics using MABA-CDP in a competition assay, overview
-
-
?
additional information
?
-
-
the enzyme shows enantioselectivity, acceptor-binding site structure of human UMP-CMP kinase in the closed form or in ligand-bound forms, overview
-
-
?
additional information
?
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hyperensitivity and resistance against anti-cancer drugs in the different cervical tumour cell lines, effects of changes on UMP/CMPK1 expression on CTP and UTP synthesis , overview
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-
?
additional information
?
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CMP and UMP are the preferred substrates for purified CMPK, and it has lower affinity to dCMP and dUMP
-
-
?
additional information
?
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mutants P64T/R134M and R134M show no activity with all substrates
-
-
?