Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.4.2: phosphomevalonate kinase

This is an abbreviated version!
For detailed information about phosphomevalonate kinase, go to the full flat file.

Word Map on EC 2.7.4.2

Reaction

ATP
+
(R)-5-phosphomevalonate
=
ADP
 +
(R)-5-diphosphomevalonate

Synonyms

5-phosphomevalonate kinase, ATP:5-phosphomevalonate phosphotransferase, CcPMK, ERG8, GbPMK, kinase, phosphomevalonate (phosphorylating), mevalonate phosphate kinase, mevalonate-5-phosphate kinase, mevalonic acid phosphate kinase, More, phosphomevalonate kinase, phosphomevalonic kinase, PMK, PMVAK, PMVK, SSO2988

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.2 phosphomevalonate kinase

Cloned

Cloned on EC 2.7.4.2 - phosphomevalonate kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in CV-1 cells
-
expressed in Escherichia coli BL21 (DE3) Rosetta cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH10B cells
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
-
expression in human embryonic kidney (HEK-293) Flp-In and CV1 cells
-
expression of phosphomevalonate kinase-green fluorescence fusion protein in CHO cells and fibroblasts
expression of the C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene CcPMK, DNA and amino acid sequence determination and analysis, cDNA on the basis of the transciptome database of Cinnamomum camphora, sequence comparison and phylogenetic analysis
gene ERG8, the pBAD plasmid containing the genes of MVK, PMK, and DMD from Saccharomyces cerevisiae (pBAD-ScMPD) is used for expression of a part of the classical MVA pathway (Fig. 6B). The codon usage of the MVK and PMK genes has been optimized for Escherichia coli, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21
gene GbPMK, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, subcloning and recombinant expression in Escherichia coli strain DH5alpha
gene PMVK, DNA and amino acid sequence determination and analysis, functional expression of soluble His-tagged wild-type enzyme and of His-tagged mutants in Escherichia coli strains JM109 and BL21(DE3)
gene PMVK, DNA and amino acid sequence determination and analysis, genotyping
gene PMVK, expression of His-tagged wild-type enzyme and mutants in Escherichia coli strain BL21(DE3)