Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.007 - 1.55
(R)-5-diphosphomevalonate
0.012 - 2.04
(R)-5-phosphomevalonate
0.007 - 1.55
(R)-mevalonate 5-diphosphate
0.034 - 2.04
(R)-mevalonate 5-phosphate
0.19
(R,S)-5-phosphomevalonate
-
-
0.042
5-phosphomevalonate
-
pH 7.2, 30°C
0.38
ADPbetaS
-
pH 7.0, 25°C
0.012
diphosphomevalonate
-
pH 7.0, 25°C
0.0042 - 0.35
phosphomevalonate
additional information
additional information
-
0.007
(R)-5-diphosphomevalonate
mutant K48M, 30°C, pH 7.0
0.041
(R)-5-diphosphomevalonate
wild-type, 30°C, pH 7.0
0.041
(R)-5-diphosphomevalonate
mutant R73M, 30°C, pH 7.0
0.044
(R)-5-diphosphomevalonate
mutant K69M, 30°C, pH 7.0
0.045
(R)-5-diphosphomevalonate
mutant R93M, 30°C, pH 7.0
0.057
(R)-5-diphosphomevalonate
mutant R138M, 30°C, pH 7.0
0.102
(R)-5-diphosphomevalonate
mutant R130M, 30°C, pH 7.0
0.139
(R)-5-diphosphomevalonate
mutant R141M, 30°C, pH 7.0
1.25
(R)-5-diphosphomevalonate
mutant R111M, 30°C, pH 7.0
1.55
(R)-5-diphosphomevalonate
mutant R84M, 30°C, pH 7.0
0.012
(R)-5-phosphomevalonate
-
pH 7.5, 30°C
0.034
(R)-5-phosphomevalonate
wild-type, 30°C, pH 7.0
0.047
(R)-5-phosphomevalonate
mutant R138M, 30°C, pH 7.0
0.048
(R)-5-phosphomevalonate
mutant R93M, 30°C, pH 7.0
0.1
(R)-5-phosphomevalonate
mutant R73M, 30°C, pH 7.0
0.102
(R)-5-phosphomevalonate
mutant R130M, 30°C, pH 7.0
0.11
(R)-5-phosphomevalonate
mutant K69M, 30°C, pH 7.0
0.131
(R)-5-phosphomevalonate
mutant K48M, 30°C, pH 7.0
0.225
(R)-5-phosphomevalonate
mutant R141M, 30°C, pH 7.0
0.88
(R)-5-phosphomevalonate
at pH 7.2 and 37°C
0.885
(R)-5-phosphomevalonate
at pH 7.2 and 30°C
1.71
(R)-5-phosphomevalonate
mutant R84M, 30°C, pH 7.0
2.04
(R)-5-phosphomevalonate
mutant R111M, 30°C, pH 7.0
0.007
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant K48M
0.041
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant wild-type enzyme
0.041
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant wild-type enzyme
0.041
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant R73M
0.044
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant K69M
0.045
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant R93M
0.047
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant R18Q
0.057
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant R138M
0.079
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant K19M
0.102
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant R130M
0.138
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant D23N
0.139
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant R141M
0.187
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant K17M
1.25
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant R111M
1.55
(R)-mevalonate 5-diphosphate
pH 7.0, 30°C, recombinant mutant R84M
0.034
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant wild-type enzyme
0.034
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant wild-type enzyme
0.047
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant R138M
0.048
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant R93M
0.1
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant R73M
0.102
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant R130M
0.11
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant K69M
0.116
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant D23N
0.123
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant R18Q
0.131
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant K48M
0.176
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant K17M
0.225
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant R141M
0.23
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant K19M
1.71
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant R84M
2.04
(R)-mevalonate 5-phosphate
pH 7.0, 30°C, recombinant mutant R111M
0.047
ADP
pH 7.0, 30°C, recombinant wild-type enzyme
0.047
ADP
pH 7.0, 30°C, recombinant wild-type enzyme
0.047
ADP
wild-type, 30°C, pH 7.0
0.06
ADP
pH 7.0, 30°C, recombinant mutant K48M
0.06
ADP
mutant K48M, 30°C, pH 7.0
0.065
ADP
pH 7.0, 30°C, recombinant mutant R18Q
0.079
ADP
pH 7.0, 30°C, recombinant mutant D23N
0.091
ADP
pH 7.0, 30°C, recombinant mutant R93M
0.091
ADP
mutant R93M, 30°C, pH 7.0
0.093
ADP
pH 7.0, 30°C, recombinant mutant R111M
0.093
ADP
mutant R111M, 30°C, pH 7.0
0.106
ADP
pH 7.0, 30°C, recombinant mutant K69M
0.106
ADP
mutant K69M, 30°C, pH 7.0
0.118
ADP
pH 7.0, 30°C, recombinant mutant R73M
0.118
ADP
mutant R73M, 30°C, pH 7.0
0.131
ADP
pH 7.0, 30°C, recombinant mutant K19M
0.164
ADP
pH 7.0, 30°C, recombinant mutant R130M
0.164
ADP
mutant R130M, 30°C, pH 7.0
0.238
ADP
pH 7.0, 30°C, recombinant mutant R84M
0.238
ADP
mutant R84M, 30°C, pH 7.0
0.286
ADP
pH 7.0, 30°C, recombinant mutant K17M
0.451
ADP
pH 7.0, 30°C, recombinant mutant R138M
0.451
ADP
mutant R138M, 30°C, pH 7.0
5.62
ADP
pH 7.0, 30°C, recombinant mutant R141M
5.62
ADP
mutant R141M, 30°C, pH 7.0
0.043
ATP
-
pH 7.5, 30°C
0.043
ATP
-
ATP in form of MgATP2-
0.0743
ATP
at pH 7.2 and 37°C
0.094
ATP
pH 7.0, 30°C, recombinant mutant R73M
0.094
ATP
mutant R73M, 30°C, pH 7.0
0.0983
ATP
at pH 7.2 and 30°C
0.101
ATP
pH 7.0, 30°C, recombinant mutant R93M
0.101
ATP
mutant R93M, 30°C, pH 7.0
0.107
ATP
pH 7.0, 30°C, recombinant wild-type enzyme
0.107
ATP
pH 7.0, 30°C, recombinant wild-type enzyme
0.107
ATP
wild-type, 30°C, pH 7.0
0.107
ATP
ATP in form of MgATP2-
0.229
ATP
pH 7.0, 30°C, recombinant mutant R130M
0.229
ATP
mutant R130M, 30°C, pH 7.0
0.236
ATP
pH 7.0, 30°C, recombinant mutant K48M
0.236
ATP
mutant K48M, 30°C, pH 7.0
0.268
ATP
pH 7.0, 30°C, recombinant mutant K69M
0.268
ATP
mutant K69M, 30°C, pH 7.0
0.303
ATP
pH 7.0, 30°C, recombinant mutant R84M
0.303
ATP
mutant R84M, 30°C, pH 7.0
0.518
ATP
pH 7.0, 30°C, recombinant mutant R138M
0.518
ATP
mutant R138M, 30°C, pH 7.0
0.536
ATP
pH 7.0, 30°C, recombinant mutant R18Q
0.536
ATP
ATP in form of MgATP2-
0.556
ATP
pH 7.0, 30°C, recombinant mutant K17M
0.556
ATP
ATP in form of MgATP2-
0.792
ATP
pH 7.0, 30°C, recombinant mutant R111M
0.792
ATP
mutant R111M, 30°C, pH 7.0
0.815
ATP
pH 7.0, 30°C, recombinant mutant D23N
0.815
ATP
ATP in form of MgATP2-
1.312
ATP
pH 7.0, 30°C, recombinant mutant K19M
1.312
ATP
ATP in form of MgATP2-
5.2
ATP
pH 7.0, 30°C, recombinant mutant R141M
5.2
ATP
mutant R141M, 30°C, pH 7.0
0.0042
phosphomevalonate
-
pH 7.0, 25°C
0.0082
phosphomevalonate
-
pH 7.5, 30°C
0.075
phosphomevalonate
-
-
0.35
phosphomevalonate
-
pH 7.5, 30°C
additional information
additional information
steady-state kinetics
-
additional information
additional information
-
steady-state kinetics
-
additional information
additional information
-
detailed kinetics, Bi Bi sequential ordered mechanism with phosphomevalonate as the first substrate and ADP the last product, the enzyme follows hyperbolic kinetics and a sequential mechanism, initial velocity and isotope exchange at equilibrium measurements, overview
-
additional information
additional information
-
kinetics of recombinant wild-type and mutant enzymes, overview
-
additional information
additional information
kinetics of recombinant wild-type and mutant enzymes, overview
-