2.7.4.22: UMP kinase
This is an abbreviated version!
For detailed information about UMP kinase, go to the full flat file.
Reaction
Synonyms
ATP:UMP phosphotransferase, HP0777 protein, More, pyrH, Rv2883c, SsUMPK, UMP kinase, UMP-kinase, UMPK, UMPKs, uridine monophosphate kinase, uridylate kinase, XC1936
ECTree
2.7.4.22 UMP kinaseAdvanced search results
results (
results (Cloned
Cloned on EC 2.7.4.22 - UMP kinase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli BL21 (DE3)-pLysS, overexpression of the Hig6-tagged target protein is induced by the addition of 0.5 mM IPTG at 20°C for 21 h, SeMet-substituted XC1936 is expressed in a similar way
-
expression in Escherichia coli, site directed mutagenesis
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
expression of the His-tagged enzyme in Escherichia coli strain B834(DE3)
Q81S73
gene pyrH or Rv2883c, sequence comparisons and phylogenetic analysis and tree
gene pyrH, DNA and amino acid sequence determination, analysis, and comparisons
gene pyrH, recombinant expression in Escherichia coli strain BL21 (DE3) from vector pT73.3
-
His-tagged version expressed in Escherichia coli BL21(DE3)
overexpression in Escherichia coli wild type and SeMet-substituted protein
-
overexpression of N-terminally His6-tagged SeMet-substituted XC1936 apo-form in Escherichia coli strain BL21(DE3)
-
plasmid vector pUHE23-2, expression in Escherichia coli strain NF1830
the mutants UpUMPKF133N and UpUMPK-F133A are constructed by site-directed mutagenesis using the plasmid pET-14b containing cDNA for UMPK, expression in Escherichia coli BL21 (DE3), overexpression by induction with isopropyl-beta-D-thiogalactoside
