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2.7.4.22: UMP kinase

This is an abbreviated version!
For detailed information about UMP kinase, go to the full flat file.

Reaction

ATP
+
UMP
=
ADP
+
UDP

Synonyms

ATP:UMP phosphotransferase, HP0777 protein, More, pyrH, Rv2883c, SsUMPK, UMP kinase, UMP-kinase, UMPK, UMPKs, uridine monophosphate kinase, uridylate kinase, XC1936

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.22 UMP kinase

Purification

Purification on EC 2.7.4.22 - UMP kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE cellulose column chromatography, Sephadex G-200 gel filtration, and nickel metal chelate affinity chromatography
heating at 70°C, ammonium sulfate precipitation, and column chromatography on Dyematrex Gel Red A and the anion exchange materials DE52 and Q6. Minor amounts (around 2%) of a smaller 15 kDa protein, presumably an SsUMPK degradation product, co-purified with SsUMP.
immobilized metal-affinity chromatography (IMAC) on a nickel column, SDS-PAGE, further purified by FPLC on an anion-exchange column, greater than 99% purity
-
metal affinity column using the gravity flow procedure, purity determined by SDS-PAGE
Ni-NTA affinity chromatography and gel filtration
Q81S73
Ni-NTA column chromatography
of the recombinant protein
of the recombinant protein by affinity chromatography
of the recombinant protein by affinity chromatography, no difference in activity compared to the wild type
of the recombinant proteins
-
recombinant His-tagged enzyme from Escherichia coli strain B834(DE3) by nickel affinity chromatography and gel filtration
Q81S73
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant N-terminally His6-tagged SeMet-substituted XC1936 apo-form from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, the His-tag is cleaved off by tobacco etch virus protease
-
TALON resin column chromatography and Superdex 200 gel filtration
the recombinant enzyme from Escherichia coli
to homogeneity
wild-type and mutant D159N