2.7.4.22: UMP kinase
This is an abbreviated version!
For detailed information about UMP kinase, go to the full flat file.
Reaction
Synonyms
ATP:UMP phosphotransferase, HP0777 protein, More, pyrH, Rv2883c, SsUMPK, UMP kinase, UMP-kinase, UMPK, UMPKs, uridine monophosphate kinase, uridylate kinase, XC1936
ECTree
Advanced search results
Purification
Purification on EC 2.7.4.22 - UMP kinase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
ammonium sulfate precipitation, DEAE cellulose column chromatography, Sephadex G-200 gel filtration, and nickel metal chelate affinity chromatography
heating at 70°C, ammonium sulfate precipitation, and column chromatography on Dyematrex Gel Red A and the anion exchange materials DE52 and Q6. Minor amounts (around 2%) of a smaller 15 kDa protein, presumably an SsUMPK degradation product, co-purified with SsUMP.
immobilized metal-affinity chromatography (IMAC) on a nickel column, SDS-PAGE, further purified by FPLC on an anion-exchange column, greater than 99% purity
-
metal affinity column using the gravity flow procedure, purity determined by SDS-PAGE
of the recombinant protein
of the recombinant protein by affinity chromatography
of the recombinant protein by affinity chromatography, no difference in activity compared to the wild type
recombinant His-tagged enzyme from Escherichia coli strain B834(DE3) by nickel affinity chromatography and gel filtration
Q81S73
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant N-terminally His6-tagged SeMet-substituted XC1936 apo-form from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, the His-tag is cleaved off by tobacco etch virus protease
-
TALON resin column chromatography and Superdex 200 gel filtration
-
of the recombinant protein by affinity chromatography, no difference in activity compared to the wild type
-
of the recombinant protein by affinity chromatography, no difference in activity compared to the wild type