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0.00014
-
R62H/D77N mutant, without UTP, no activity with 1 mM UTP
0.0002
-
R62H mutant, without UTP, no activity with 1 mM UTP
0.0003
-
D77N mutant, without UTP, no activity with 1 mM UTP
0.00613
-
wild-type, with 1 mM UTP
0.011
0-45% ammonium sulfate precipitate
0.01238
-
wild-type, without UTP
0.033
45-55% ammonium sulfate precipitate
0.053
55-80% ammonium sulfate precipitate
0.13
mutant D201N, rate of the reverse reaction
0.15
mutant D146N, at pH 6
0.32
mutant D146N, at pH 8
0.6
with 6-aza-UMP as substrate
1.04
mutant D201N, at pH 6
1.7
-
at 2.0 mM ATP, without GTP
10.2
at 8.0 mM ATP, with 0.5 mM GTP
11.3
at 8.0 mM ATP, without GTP
11.8
-
at 8.0 mM ATP, without GTP
110.5
N140A/D159N mutant protein, 0.3 mM UMP, 0.5 mM GTP
12.8
-
at 8.0 mM ATP, without GTP
124.2
-
at 8.0 mM ATP, with 0.5 mM GTP
126
with UMP as substrate
130
with MnCl2 and ATP as phosphate donor
134.3
-
at 2.0 mM ATP, with 0.5 mM GTP
135
at pH 6.5, in the presence of MnCl2
160
wild-type, in the presence of ATP and GTP
162
with 5-fluoro-UMP as substrate
18.1
at 8.0 mM ATP, without GTP
182
at pH 6.5, in the presence of MgCl2
19.4
-
at 8.0 mM ATP, with 0.5 mM GTP
2.7
at 2.0 mM ATP, without GTP
23.5
-
at 2.0 mM ATP, with 0.5 mM GTP
23.6
-
at 8.0 mM ATP, with 0.5 mM GTP
23.8
D159N mutant protein, 0.3 mM UMP, 0.5 mM UTP
24
with 5-fluoro-UMP as substrate
26.1
at 2.0 mM ATP, without GTP
274
at pH 7.5, in the presence of MnCl2
28.1
at 8.0 mM ATP, without GTP
28.6
at 2.0 mM ATP, with 0.5 mM GTP
3.6
mutant D201N, in the presence of 1 mM ATP and 1 mM UMP
3.7
-
at 2.0 mM ATP, without GTP
30.4
N140A/D159N mutant protein, 0.2 mM ATP, 0.5 mM UTP
36.3
D93A/D159N mutant protein, 0.2 mM ATP, 0.5 mM GTP, 0.5 mM UTP
36.8
D159N mutant protein, 0.2 mM ATP, 0.5 mM UTP
38.1
D93A/D159N mutant protein, 0.2 mM ATP, 0.5 mM UTP
38.4
D93A/D159N mutant protein, 0.2 mM ATP, 0.5 mM GTP
38.9
-
at 8.0 mM ATP, without GTP
39.1
at 8.0 mM ATP, with 0.5 mM GTP
399
at pH 7.5, in the presence of MgCl2
4.6
-
at 2.0 mM ATP, without GTP
40.7
D93A/D159N mutant protein, 0.2 mM ATP
46.1
D159N mutant protein, 0.2 mM ATP
46.6
D159N mutant protein, 0.2 mM ATP, 0.5 mM GTP, 0.5 mM UTP
46.8
-
activity independent of concentrations of ATP, at 2.0 mM ATP, without GTP
47.5
-
at 8.0 mM ATP, without GTP
48.9
N140A/D159N mutant protein, 0.2 mM ATP
5.1
activity dependent of concentrations of ATP, at 2.0 mM ATP, without GTP
51.4
N72A/D93A/D159N mutant protein, 2 mM ATP, 0.5 mM GTP
51.9
D159N mutant protein, 0.2 mM ATP, 0.5 mM GTP
52.7
N72A/D93A/D159N mutant protein, 2 mM ATP
52.8
-
at 2.0 mM ATP, with 0.5 mM GTP
55.7
N140A/D159N mutant protein, 0.2 mM ATP, 0.5 mM GTP, 0.5 mM UTP
57.6
D93A/D159N mutant protein, 0.3 mM UMP, 0.5 mM GTP, 0.5 mM UTP
57.7
at 2.0 mM ATP, with 0.5 mM GTP
58.2
N140A/D159N mutant protein, 0.2 mM ATP, 0.5 mM GTP
60.5
-
at 8.0 mM ATP, with 0.5 mM GTP
60.8
D93A/D159N mutant protein, 0.3 mM UMP, 0.5 mM UTP
61.5
D93A/D159N mutant protein, 0.3 mM UMP
62.3
D93A/D159N mutant protein, 0.3 mM UMP, 0.5 mM GTP
62.4
D159N mutant protein, 0.3 mM UMP
65.9
at 2.0 mM ATP, with 0.5 mM GTP
66
with MgCl2 and ATP as phosphate donor
666
at pH 8.5, in the presence of MnCl2
67
with 6-aza-UMP as substrate
68.5
-
at 2.0 mM ATP, with 0.5 mM GTP
71.1
at 8.0 mM ATP, with 0.5 mM GTP
76.1
N72A/D93A/D159N mutant protein, 1 mM UMP, 0.5 mM GTP
77.1
D93A/D159N mutant protein, 2 mM ATP
78.3
N140A/D159N mutant protein, 0.3 mM UMP, 0.5 mM UTP
78.7
N72A/D93A/D159N mutant protein, 0.3 mM UMP
81.9
N140A/D159N mutant protein, 0.3 mM UMP
83.4
D93A/D159N mutant protein, 2 mM ATP, 0.5 mM GTP
86.6
mutant D168N, at pH 8
87.4
-
at 8.0 mM ATP, with 0.5 mM GTP
9.7
-
at 2.0 mM ATP, with 0.5 mM GTP
90.7
D93A/D159N mutant protein, 0.3 mM UMP
91.7
D159N mutant protein, 0.3 mM UMP, 0.5 mM GTP, 0.5 mM UTP
93.9
D93A/D159N mutant protein, 1 mM UMP, 0.5 mM GTP
98.2
N140A/D159N mutant protein, 0.3 mM UMP, 0.5 mM GTP, 0.5 mM UTP
99.6
D159N mutant protein, 0.3 mM UMP, 0.5 mM GTP
0.003
-
100.3
D159N mutant protein, 2 mM ATP, 0.5 mM GTP
100.3
N72A/D159N mutant protein, 2 mM ATP, 0.5 mM GTP
105
wild-type, at pH 6
128
mutant D159N, at pH 6
128
mutant D159N, at pH 6.0
153
mutant D159N, at pH 7.4
153
mutant D159N, at pH 8
61.6
D159N mutant protein, 0.3 mM UMP
61.6
N72A/D159N mutant protein, 0.3 mM UMP
8.2
mutant D201N, at pH 8
84.2
D159N mutant protein, 1 mM UMP, 0.5 mM GTP
84.2
N72A/D159N mutant protein, 1 mM UMP, 0.5 mM GTP
9.1
-
at 8.0 mM ATP, without GTP
9.1
-
activity independent of concentrations of ATP, at 2.0 mM ATP, without GTP
92.5
D159N mutant protein, 2 mM ATP
92.5
N72A/D159N mutant protein, 2 mM ATP
additional information
R92A, H96A, and R127A exhibit increased enzyme activity
additional information
-
R92A, H96A, and R127A exhibit increased enzyme activity
additional information
GTP and UTP are extremely poor substrates with an activity below 1 micromol/min/mg with MnCl2 or MgCl2
additional information
-
GTP and UTP are extremely poor substrates with an activity below 1 micromol/min/mg with MnCl2 or MgCl2