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2.7.4.22: UMP kinase

This is an abbreviated version!
For detailed information about UMP kinase, go to the full flat file.

Reaction

ATP
+
UMP
=
ADP
+
UDP

Synonyms

ATP:UMP phosphotransferase, HP0777 protein, More, pyrH, Rv2883c, SsUMPK, UMP kinase, UMP-kinase, UMPK, UMPKs, uridine monophosphate kinase, uridylate kinase, XC1936

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.22 UMP kinase

Specific Activity

Specific Activity on EC 2.7.4.22 - UMP kinase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00014
-
R62H/D77N mutant, without UTP, no activity with 1 mM UTP
0.0002
-
R62H mutant, without UTP, no activity with 1 mM UTP
0.0003
-
D77N mutant, without UTP, no activity with 1 mM UTP
0.003
0.00613
-
wild-type, with 1 mM UTP
0.011
0-45% ammonium sulfate precipitate
0.01238
-
wild-type, without UTP
0.033
45-55% ammonium sulfate precipitate
0.053
55-80% ammonium sulfate precipitate
0.13
mutant D201N, rate of the reverse reaction
0.15
mutant D146N, at pH 6
0.32
mutant D146N, at pH 8
0.6
with 6-aza-UMP as substrate
1.04
mutant D201N, at pH 6
1.5
mutant R62H, at pH 6
1.7
-
at 2.0 mM ATP, without GTP
1.9
mutant D77N, at pH 6
10.2
at 8.0 mM ATP, with 0.5 mM GTP
100.3
11.3
at 8.0 mM ATP, without GTP
11.8
-
at 8.0 mM ATP, without GTP
110.5
N140A/D159N mutant protein, 0.3 mM UMP, 0.5 mM GTP
12.8
-
at 8.0 mM ATP, without GTP
124.2
-
at 8.0 mM ATP, with 0.5 mM GTP
126
with UMP as substrate
130
with MnCl2 and ATP as phosphate donor
134.3
-
at 2.0 mM ATP, with 0.5 mM GTP
135
at pH 6.5, in the presence of MnCl2
160
wild-type, in the presence of ATP and GTP
162
with 5-fluoro-UMP as substrate
18.1
at 8.0 mM ATP, without GTP
182
at pH 6.5, in the presence of MgCl2
19.4
-
at 8.0 mM ATP, with 0.5 mM GTP
2.7
at 2.0 mM ATP, without GTP
23.5
-
at 2.0 mM ATP, with 0.5 mM GTP
23.6
-
at 8.0 mM ATP, with 0.5 mM GTP
23.8
D159N mutant protein, 0.3 mM UMP, 0.5 mM UTP
24
with 5-fluoro-UMP as substrate
25
His-tagged protein
26
native protein
26.1
at 2.0 mM ATP, without GTP
274
at pH 7.5, in the presence of MnCl2
28.1
at 8.0 mM ATP, without GTP
28.6
at 2.0 mM ATP, with 0.5 mM GTP
3.6
mutant D201N, in the presence of 1 mM ATP and 1 mM UMP
3.7
-
at 2.0 mM ATP, without GTP
30.4
N140A/D159N mutant protein, 0.2 mM ATP, 0.5 mM UTP
32
mutant D174N, at pH 6
36.3
D93A/D159N mutant protein, 0.2 mM ATP, 0.5 mM GTP, 0.5 mM UTP
36.8
D159N mutant protein, 0.2 mM ATP, 0.5 mM UTP
38.1
D93A/D159N mutant protein, 0.2 mM ATP, 0.5 mM UTP
38.4
D93A/D159N mutant protein, 0.2 mM ATP, 0.5 mM GTP
38.9
-
at 8.0 mM ATP, without GTP
39.1
at 8.0 mM ATP, with 0.5 mM GTP
399
at pH 7.5, in the presence of MgCl2
4.4
mutant R62H, at pH 8
4.6
-
at 2.0 mM ATP, without GTP
40.7
D93A/D159N mutant protein, 0.2 mM ATP
45
mutant D174N, at pH 8
46.1
D159N mutant protein, 0.2 mM ATP
46.6
D159N mutant protein, 0.2 mM ATP, 0.5 mM GTP, 0.5 mM UTP
46.8
-
activity independent of concentrations of ATP, at 2.0 mM ATP, without GTP
48.9
N140A/D159N mutant protein, 0.2 mM ATP
5.1
activity dependent of concentrations of ATP, at 2.0 mM ATP, without GTP
51.4
N72A/D93A/D159N mutant protein, 2 mM ATP, 0.5 mM GTP
51.9
D159N mutant protein, 0.2 mM ATP, 0.5 mM GTP
52
mutant N140A
52.7
N72A/D93A/D159N mutant protein, 2 mM ATP
52.8
-
at 2.0 mM ATP, with 0.5 mM GTP
55.7
N140A/D159N mutant protein, 0.2 mM ATP, 0.5 mM GTP, 0.5 mM UTP
57.6
D93A/D159N mutant protein, 0.3 mM UMP, 0.5 mM GTP, 0.5 mM UTP
57.7
at 2.0 mM ATP, with 0.5 mM GTP
58.2
N140A/D159N mutant protein, 0.2 mM ATP, 0.5 mM GTP
60.5
-
at 8.0 mM ATP, with 0.5 mM GTP
60.8
D93A/D159N mutant protein, 0.3 mM UMP, 0.5 mM UTP
61.5
D93A/D159N mutant protein, 0.3 mM UMP
62.3
D93A/D159N mutant protein, 0.3 mM UMP, 0.5 mM GTP
62.4
D159N mutant protein, 0.3 mM UMP
63
mutant D168N, at pH 6
65.9
at 2.0 mM ATP, with 0.5 mM GTP
66
with MgCl2 and ATP as phosphate donor
666
at pH 8.5, in the presence of MnCl2
67
with 6-aza-UMP as substrate
68.5
-
at 2.0 mM ATP, with 0.5 mM GTP
71.1
at 8.0 mM ATP, with 0.5 mM GTP
76.1
N72A/D93A/D159N mutant protein, 1 mM UMP, 0.5 mM GTP
77.1
D93A/D159N mutant protein, 2 mM ATP
78.3
N140A/D159N mutant protein, 0.3 mM UMP, 0.5 mM UTP
78.7
N72A/D93A/D159N mutant protein, 0.3 mM UMP
81.9
N140A/D159N mutant protein, 0.3 mM UMP
83.4
D93A/D159N mutant protein, 2 mM ATP, 0.5 mM GTP
86.6
mutant D168N, at pH 8
87.4
-
at 8.0 mM ATP, with 0.5 mM GTP
88
mutant T138A
9.7
-
at 2.0 mM ATP, with 0.5 mM GTP
90
mutant T138A/N140A
90.7
D93A/D159N mutant protein, 0.3 mM UMP
91.7
D159N mutant protein, 0.3 mM UMP, 0.5 mM GTP, 0.5 mM UTP
93.9
D93A/D159N mutant protein, 1 mM UMP, 0.5 mM GTP
98.2
N140A/D159N mutant protein, 0.3 mM UMP, 0.5 mM GTP, 0.5 mM UTP
99.6
D159N mutant protein, 0.3 mM UMP, 0.5 mM GTP
additional information