2.7.4.25: (d)CMP kinase

This is an abbreviated version!
For detailed information about (d)CMP kinase, go to the full flat file.

Reaction

Synonyms

ATP:CMP phosphotransferase, bacterial cytidylate kinase, CMK, CMP kinase, CMPK, cytidine 5�-monophosphate kinase, cytidine monophosphate kinase, cytidylate kinase, dCMP kinase, deoxycytidine monophosphate kinase, deoxycytidine monophosphokinase, deoxycytidylate kinase, More, P25 protein, prokaryotic cytidylate kinase, SP1603, UCK, UMP-CMP kinase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.4 Phosphotransferases with a phosphate group as acceptor

                2.7.4.25 (d)CMP kinase

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Results	in table
41245	AA Sequence
1	Application
12	Cloned(Commentary)
10	Cofactor
7	Crystallization (Commentary)
5	General Information
1	General Stability
1	Inhibitors
38	kcat/KM [mM/s]
98	KM Value [mM]
5	Metals/Ions
7	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
210	Organism
4	Pathway
9	PDB ID
25	Protein Variants
8	Purification (Commentary)
1	Reaction
3	Reaction Type
19	Reference
4	Specific Activity [micromol/min/mg]
2	Storage Stability
76	Substrates and Products (Substrate)
5	Subunits
44	Synonyms
1	Systematic Name
15	Temperature Stability [°C]
45	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 2.7.4.25 - (d)CMP kinase

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.083 - 105

ara-CMP

4 entries

0.0114 - 2940

CMP

13 entries

0.0068 - 1160

dCMP

13 entries

0.0033 - 1.25

UMP

8 entries

0.083

ara-CMP

Escherichia coli K-12

-

mutant enzyme D185A, at 30°C and pH 7.4

712736

1.7

ara-CMP

Escherichia coli K-12

-

mutant enzyme R181M, at 30°C and pH 7.4

712736

7.5

ara-CMP

Escherichia coli K-12

-

mutant enzyme S101A, at 30°C and pH 7.4

712736

105

ara-CMP

Escherichia coli K-12

-

wild type enzyme, at 30°C and pH 7.4

712736

0.0114

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme R110M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.053

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132H, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.12

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme R188M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.54

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132N, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.54

CMP

Escherichia coli K-12

-

mutant enzyme D185A, at 30°C and pH 7.4

712736

1.41

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

7.4

CMP

Escherichia coli K-12

-

mutant enzyme R181M, at 30°C and pH 7.4

712736

25.2

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme S36A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

589

CMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132S, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

697

CMP

Escherichia coli K-12

-

mutant enzyme S101A, at 30°C and pH 7.4

712736

1100

CMP

Mycobacterium tuberculosis

-

pH and temperature not specified in the publication

712276

2940

CMP

Escherichia coli K-12

-

wild type enzyme, at 30°C and pH 7.4

712736

2940

CMP

Escherichia coli K-12

P0A6I0

wild type enzyme, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.0068

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme R110M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.052

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme R188M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.3

dCMP

Escherichia coli K-12

-

mutant enzyme D185A, at 30°C and pH 7.4

712736

0.4

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

1.1

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132H, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

1.88

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132N, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

1.9

dCMP

Escherichia coli K-12

-

mutant enzyme R181M, at 30°C and pH 7.4

712736

4.1

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme S36A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

6.1

dCMP

Escherichia coli K-12

-

mutant enzyme S101A, at 30°C and pH 7.4

712736

200

dCMP

Mycobacterium tuberculosis

-

pH and temperature not specified in the publication

712276

230

dCMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132S, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

1150

dCMP

Escherichia coli K-12

P0A6I0

wild type enzyme, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

1160

dCMP

Escherichia coli K-12

-

wild type enzyme, at 30°C and pH 7.4

712736

0.0033

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132H, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.0048

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme R110M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.083

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132N, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.3

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme S36A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

0.88

UMP

Mycobacterium tuberculosis

-

pH and temperature not specified in the publication

712276

0.88

UMP

Escherichia coli K-12

P0A6I0

wild type enzyme, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

1.04

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132S, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282

1.25

UMP

Escherichia coli K-12

P0A6I0

mutant enzyme D132A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C

692282