2.7.4.4: nucleoside-phosphate kinase

This is an abbreviated version!
For detailed information about nucleoside-phosphate kinase, go to the full flat file.

Reaction

Synonyms

AMPK1, AMPK2, kinase, nucleoside monophosphate (phosphorylating), NMP kinase, NMP-kinase, nucleoside monophosphate kinase, thymidylate kinase, TMPK

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.4 Phosphotransferases with a phosphate group as acceptor

                2.7.4.4 nucleoside-phosphate kinase

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Results	in table
2	Activating Compound
2	AA Sequence
1	CAS Registry Number
5	Disease/ Diagnostics
6	Inhibitors
4	kcat/KM [mM/s]
4	KM Value [mM]
1	Localization
4	Metals/Ions
1	Natural Substrates/ Products (Substrates)
4	Organism
6	PDB ID
1	pH Range
2	pH Stability
2	Purification (Commentary)
1	Reaction
1	Reaction Type
4	Reference
2	Source Tissue
1	Specific Activity [micromol/min/mg]
2	Storage Stability
9	Substrates and Products (Substrate)
9	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Temperature Stability [°C]
4	Turnover Number [1/s]

KM Value

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KM Value on EC 2.7.4.4 - nucleoside-phosphate kinase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.08 - 0.14

dAMP

2 entries

0.23

TMP

Homo sapiens

-

in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C

722049

0.119

[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid

Homo sapiens

-

in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C

722049

0.08

dAMP

Homo sapiens

-

isoform AMPK2, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37°C

722061

0.14

dAMP

Homo sapiens

-

isoform AMPK1, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37°C

722061