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2.7.4.6: nucleoside-diphosphate kinase

This is an abbreviated version!
For detailed information about nucleoside-diphosphate kinase, go to the full flat file.

Word Map on EC 2.7.4.6

Reaction

ATP
+
nucleoside diphosphate
=
ADP
+
nucleoside triphosphate

Synonyms

AfNDK, Afu5g03490, ASNDK, At4g09320, At4g11010, At5g63310, HsNDK, kinase, nucleoside diphosphate (phosphorylating), mitochondrial nucleoside diphosphate kinase, MJ1265, More, NDK, NDK B, NDK-1, NDKB, NDP kinase, NDP kinase A, NDP kinase alpha, NDP kinase beta, NDPK, NDPK B, NDPK I, NDPK II, NDPK III, NDPK IIpn, NDPK In, NDPK-3, NDPK-B, NDPK-C, NDPK-D, NDPK-In, NDPK1, NDPK2, NDPK3, NDPK3a, NDPKA, NDPKB, NM23, NM23 metastasis suppressor, Nm23-H1, NM23-H2, NM23-H4, NME1, NME2, NME3, NME4, nucleoside 5'-diphosphate kinase, nucleoside diphosphate (UDP) kinase, nucleoside diphosphate kinase, nucleoside diphosphate kinase 1, nucleoside diphosphate kinase 2, nucleoside diphosphate kinase 3, nucleoside diphosphate kinase 3a, nucleoside diphosphate kinase A, nucleoside diphosphate kinase B, nucleoside diphosphate kinase C, nucleoside diphosphate kinase D, nucleoside diphosphate kinase-1, nucleoside diphosphate kinase-2, nucleoside diphosphate kinases alpha, nucleoside diphosphate kinases beta, nucleoside diphosphate phosphotransferase, nucleoside diphosphokinase, nucleoside-diphosphate kinase 3, nucleotide diphosphate kinase, nucleotide phosphate kinase, ORF454, Os12g36194, OsNDPK2, SwoH, TcNDPK1, TcNDPK3, UDP kinase, uridine diphosphate kinase, WSL12, YSS2 protein

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.6 nucleoside-diphosphate kinase

Cloned

Cloned on EC 2.7.4.6 - nucleoside-diphosphate kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned and expressed in Escherichia coli
cloned and expressed in Escherichia coli JM83
-
cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme
cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme (NDPK)
cytosolic form
expressed as GST-fusion protein in Escherichia coli
expressed as HAT-tag fusion protein in Escherichia coli
expressed as His-tag fusion protein in Escherichia coli
-
expressed as His-tag fusion protein in Escherichia coli DH5alpha
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21 Star (DE3) cells
-
expressed in Escherichia coli BL21 Star(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLyS cells
-
expressed in Escherichia coli BL21(DE3)pLysE
-
expressed in Escherichia coli BL21star(DE3) cells
expressed in Escherichia coli JM109 cells
-
expressed in Escherichia coli M15 cells
-
expressed in Escherichia coli Tuner(DE3)pLysS
-
expressed with and without His-tag in Escherichia coli
-
expression in Escherichia coli
expression in Escherichia coli as His-tagged protein
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli BL21(DE3)R3 Rosetta cells
expression in Escherichia coli or in Saccharomyces cerevisiae
expression of GST-fusion NM23 protein in Escherichia coli
-
expression of GST-tagged NPDK2
expression of HA-tagged chimeric mutants NDP kinase alpha1-130beta131-152 and NDP kinase beta1-130alpha131-152
-
expression of His-tagged NDPK in Escherichia coli
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109
expression of His-tagged wild-type and mutant NDPKB
-
expression of His6-tagged enzyme containing a thrombin cleavage site
-
expression of isozyme NDPK2 in Solanum tuberosum cv. Atlantic plant leaf cytosol under the control of an oxidative stress-inducible SWPA2 promoter or enhanced CaMV 35S promoter, resulting in transgenic lines SN1, SN19 and EN1, EN2
expression of wild-type and mutant enzymes
functional recombinant expression in Escherichia coli
fusion of extra sequence containing hexa-His-tag at amino-terminus of the enzyme facilitates folding and activation in Escherichia coli
gene awd, DNA and amino acid sequence determinatioon and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene Cf-ndpk, DNA and amino acid sequence determination and analysis, sequence comparison, functional expression in Escherichia coli, the recombinant enzyme shows transfer of the terminal phosphate between UDP and ATP
gene ndk, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene ndk, expression of wild-type and mutant enzymes in Escherichia coli strain JM109
gene ndk, functional expression of untagged wild-type enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene ndk, overexpressionin strain BL21(DE3)
-
gene ndk, recombinant expression in Escherichia coli strain BL21Star(DE3)
-
gene ndk, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
-
gene ndk, recombinant expression of tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene ndk, recombinant expression of wild-type and mutant enzymes, quantitative real-time PCR expression analysis
gene ndk, sequence comparison
gene ndk, sequence comparison, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21Star (DE3) , subcloning in Escherichia coli strains JM109 and DH5alpha
gene ndpk2, expression of GST-tagged wild-type enzyme in Escherichia coli, expression of SOS2 and NDPK2 in Saccharomyces cerevisiae in the two-hybrid system
-
gene NDPK3 or At4g11010, enzyme overexpression in transgenic Arabidopsis thalina a lines, quantitative RT-PCR expression analysis
gene NDPK3a, expression analysis, NDPK3a transcript abundance in leaves treated with different sugars and NAG, overview
-
gene TcCLB.508707.200, recombinant overexpression of GFP-tagged wild-type and mutant enzymes in transgenic epimastigotes, expression analysis, enzyme fused with dimeric GFP relocalizes into protein granules inside the cell, positioned in most cases adjacent to the nucleus, while control parasites transfected with GFP conserved the cytosolic localization, overview. The observed granules all along the parasites population are of different size probably due to the heterogeneity in the expression levels of the fusion protein. The majority of this granules occupy 5-15% of the cell body
isozyme NDK B, DNA and amino acid sequence determination and analysis, sequence comparison
isozyme NDPK3, expression in Escherichia coli
-
mutant enzyme D93N is expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells
native and mutant enzymes expressed as His-tag fusion protein in Escherichia coli SG13009
-
NDPK isozymes, sequence comparisons and phylogenetic analysis, expression of GFP-tagged isozyme NDPK3 in Arabidopsis thaliana protoplasts, targeting to the mitochondria
-
overexpressed in Escherichia coli
overexpression in H10 cell membranes
-
overview
-
recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
transient expression of GFP-tagged wild-type and mutant NDPK2s in Pisum sativum protoplasts
wild type and mutant proteins expressed as GST-fusion protein in Escherichia coli BL21(DE3) or DH5alpha, complementation studies with NdK deficient Pseudomonas aeruginosa