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2.7.4.6: nucleoside-diphosphate kinase

This is an abbreviated version!
For detailed information about nucleoside-diphosphate kinase, go to the full flat file.

Word Map on EC 2.7.4.6

Reaction

ATP
+
nucleoside diphosphate
=
ADP
 +
nucleoside triphosphate

Synonyms

AfNDK, Afu5g03490, ASNDK, At4g09320, At4g11010, At5g63310, HsNDK, kinase, nucleoside diphosphate (phosphorylating), mitochondrial nucleoside diphosphate kinase, MJ1265, More, NDK, NDK B, NDK-1, NDKB, NDP kinase, NDP kinase A, NDP kinase alpha, NDP kinase beta, NDPK, NDPK B, NDPK I, NDPK II, NDPK III, NDPK IIpn, NDPK In, NDPK-3, NDPK-B, NDPK-C, NDPK-D, NDPK-In, NDPK1, NDPK2, NDPK3, NDPK3a, NDPKA, NDPKB, NM23, NM23 metastasis suppressor, Nm23-H1, NM23-H2, NM23-H4, NME1, NME2, NME3, NME4, nucleoside 5'-diphosphate kinase, nucleoside diphosphate (UDP) kinase, nucleoside diphosphate kinase, nucleoside diphosphate kinase 1, nucleoside diphosphate kinase 2, nucleoside diphosphate kinase 3, nucleoside diphosphate kinase 3a, nucleoside diphosphate kinase A, nucleoside diphosphate kinase B, nucleoside diphosphate kinase C, nucleoside diphosphate kinase D, nucleoside diphosphate kinase-1, nucleoside diphosphate kinase-2, nucleoside diphosphate kinases alpha, nucleoside diphosphate kinases beta, nucleoside diphosphate phosphotransferase, nucleoside diphosphokinase, nucleoside-diphosphate kinase 3, nucleotide diphosphate kinase, nucleotide phosphate kinase, ORF454, Os12g36194, OsNDPK2, SwoH, TcNDPK1, TcNDPK3, UDP kinase, uridine diphosphate kinase, WSL12, YSS2 protein

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.6 nucleoside-diphosphate kinase

Renatured

Renatured on EC 2.7.4.6 - nucleoside-diphosphate kinase

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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
extremely halophilic enzyme inactivated at low salt concentrations using heat-treatment or 6 M urea, addition of or dialysis against 2 M NaCl, 3 M KCl, or 4 M trimethylamine N-oxide required for effective refolding of enzyme, refolding using 4 M trimethylamine N-oxide is strongly pH-dependent, good refolding at pH 7, almost no activity regained at pH 8, at pH 8 inclusion of low concentrations of NaCl supports refolding and activation of activity
-
in 50 mM Tris-HCl buffer containing 3 M NaCl, at 4°C. Refolding is extremely slow, reaching the nearly full activity at 72 h
-
refolding of heat-denatured wild type enzyme in 50 mM Tris-HCl (pH 7.5) occurs depending on the salt concentration. Refolding rate is slow even in the presence of 2 M NaCl, reaching maximum at 2 days
-
refolding of heat-denatured wild-type enzyme requires salt concentrations above 2 M. Mutant G114R efficiently refolds in presence of 1 M NaCl
refolding of the His-tagged enzyme after denaturation is much more efficient than refolding of the untagged enzyme
renaturation of subunit polypeptides A and B generates several isozymes
-
the NDK readily refolds and reassembles back to the active dimeric structure, upon heat denaturation at 0.2 M NaCl, as soon as the temperature is lowered
-
the wild type enzyme unfolds and inactivates simultaneously in the presence of 4 M guanidine hydrochloride as with 4 M urea as denaturant. Refolding occurs at much lower guanidine hydrochloride concentrations (0.5-1 M)