Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.4.8: guanylate kinase

This is an abbreviated version!
For detailed information about guanylate kinase, go to the full flat file.

Word Map on EC 2.7.4.8

Reaction

ATP
+
GMP
=
ADP
 +
GDP

Synonyms

5'-GMP kinase, apo-EcGMPK, ATP: GMP phosphotransferase, ATP:GMP phosphotransferase, BmGK, CASK polypeptide, Cavbeta1b, Cavbeta2a, deoxyguanylate kinase, DLG1/SAP97, ecGMPK, GK, GK3, GKc, GKpm, GMK, GMK3, GMP kinase, GMPK, guanosine monophosphate kinase, guanosine monophosphate kinase (EcGMPK), guanylate kinase, guanylate kinase (GK), guanylate monophosphate kinase, GUK, kinase, guanylate (phosphorylating), MAGUK, MAGUK PSD-95, MAGUKs, membrane associated guanylate kinase, membrane associated guanylate kinase protein, membrane-associated guanylate kinase, membrane-associated guanylate kinases, MoGuk1, MoGuk2, More, organellar GK, post-synaptic density-95 membrane associated guanylate kinase, postsynaptic density protein-95 SH3 GK, PSD-95 GK, PSD-95 MAGUK, PSD-95alpha, PSD-95beta, Staphylococcus aureus guanylate monophosphate kinase, voltage-gated calcium channel beta1b, voltage-gated calcium channel beta2a, YGK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.8 guanylate kinase

Crystallization

Crystallization on EC 2.7.4.8 - guanylate kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
vapour diffusion method
purified the GK domain fragment of human DLG1, hanging drop vapor diffusion method, mixing of 6.5 mg/ml protein in 20 mM Tris-HCl buffer, pH 8.0, 150 mM NaCl and 2 mM dithiothreitol, with reservoir solution containing 0.2 M sodium thiocyanate and 22% PEG 3350, 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement and selenomethionine single-wavelength anomalous dispersion, using the PSD-95 GK domain, PDB ID 1JXO, as a search model
SAP97 SH3-guanylate kinase/p-LGN18 complex, hanging drop vapor diffusion method, using 0.2M ammonium nitrate, 20% (w/v) polyethylene glycol 3350
-
crystal structure of a complex with ADP and GMP
-
SAP97 SH3-guanylate kinase/p-LGN18 complex, hanging drop vapor diffusion method, using 0.2M ammonium nitrate, 20% (w/v) polyethylene glycol 3350
crystal structure of a complex with GMP
-
crystal structure of the enzyme with a non-acetylated N terminus in its unligated form as well as in complex with GMP
octahedral bipyramids, preliminary X-ray analysis
-
temperature-dependent space-group transitions between orthorhombic and tetragonal forms
-
Crystals grown in 20% PEG 3350, 0.2 M LiSO4 and 0.1 M Tris-HCl pH 8.
-