2.7.6.2: thiamine diphosphokinase

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For detailed information about thiamine diphosphokinase, go to the full flat file.

Reaction

Synonyms

At1g02880 (AtTPK1), At2g44750 (AtTPK2), ATP:thiamin pyrophosphotransferase, CA1462, hTPK1, Plasmodium falciparum thiamine pyrophosphokinase, pyrophosphokinase, pyrophosphokinase, thiamin, TDPK, thiamin kinase, thiamin pyrophosphokinase, thiamin pyrophosphotransferase, thiamin:ATP pyrophosphotransferase, thiamine diphosphokinase, thiamine kinase, thiamine pyrophokinase, thiamine pyrophosphokinase, thiaminokinase, TPK, Tpk1, TPTase, YcfN

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.6 Diphosphotransferases

                2.7.6.2 thiamine diphosphokinase

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Results	in table
1	Activating Compound
14443	AA Sequence
3	Application
1	CAS Registry Number
9	Cloned(Commentary)
5	Cofactor
5	Crystallization (Commentary)
87	Disease/ Diagnostics
1	Expression
3	General Information
1	General Stability
34	Inhibitors
3	Ki Value [mM]
38	KM Value [mM]
8	Localization
22	Metals/Ions
11	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
37	Organism
8	Pathway
14	PDB ID
10	pH Optimum
3	pH Range
2	pI Value
12	Protein Variants
13	Purification (Commentary)
3	Reaction
1	Reaction Type
35	Reference
50	Source Tissue
12	Specific Activity [micromol/min/mg]
50	Substrates and Products (Substrate)
8	Subunits
48	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
4	Temperature Range [°C]
2	Temperature Stability [°C]
18	Turnover Number [1/s]

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Cloned on EC 2.7.6.2 - thiamine diphosphokinase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

Escherichia coli strain KL21 (DE3)pLysS is transformed with the expression vector coding for a histidine-tagged wild-type or mutant hTPK1

Homo sapiens

Q9H3S4

642743

expressed in Escherichia coli BL21 cells

Mus musculus

-

722395

gene CA1462, expression in Escherichia coli

Candida albicans

-

697004

gene tnr3

Schizosaccharomyces pombe

P41888

642757

gene TPK1, TPK1 Sanger sequencing analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS

Homo sapiens

Q9H3S4

739080

isozymes TPK1 and TPK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21, expression of fluorescent tagged isozymes in cytosol of mesophyll protoplasts

Arabidopsis thaliana

F4IV16

676560

overexpression in Escherichia coli

Saccharomyces cerevisiae

-

661335

TPK1

Homo sapiens

Q9H3S4

642755

TPK1 gene, located in 7q34-36, genotyping in 964 individuals from 220 families of European ancestry revealing an association between maternal rs228584 genotype and offspring birth weight

Homo sapiens

-

695592