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2.7.6.3: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase

This is an abbreviated version!
For detailed information about 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, go to the full flat file.

Word Map on EC 2.7.6.3

Reaction

ATP
+
6-hydroxymethyl-7,8-dihydropterin
=
AMP
 +
6-hydroxymethyl-7,8-dihydropterin diphosphate

Synonyms

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase, 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase/dihydropteroate synthase, 6-hydroxy-7,8-dihydropterin pyrophosphokinase, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK), 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/dihydropteroate synthase, 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase, 6-hydroxymethylpterin pyrophosphokinase, 7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase, 7,8-dihydropteroate-synthesizing enzyme, 7,8-dihydroxymethylpterin-pyrophosphokinase, ATP:2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine 6'-pyrophosphotransferase, cytHPPK/DHPS, dihydropterin pyrophosphokinase, FOLK, FtHPPK-DHPS, H2-pteridine-CH2-OH pyrophosphokinase, H2-pteridine-CH2OH pyrophosphokinase, HPPK, HPPK-DHPS, HPPK/DHPS, HPPK/dihydropteroate synthase, hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase, hydroxymethyldihydropteridine pyrophosphokinase, hydroxymethyldihydropterin diphosphokinase/dihydropteroate synthase, hydroxymethyldihydropterin pyrophosphokinase (HPPK), hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase, mitHPPK/DHPS, More, PPPK, PPPK-DHPS, PVX_123230, pyrophosphokinase, 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.6 Diphosphotransferases
                2.7.6.3 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase

Cloned

Cloned on EC 2.7.6.3 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
bifunctional protein 6-hydroxymethyl-7,8-dihydroxypterin pyrophosphokinase/7,8-dihydropteroate synthase is involved in tetrahydrofolate expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
-
expression in Escherichia coli
expression in Escherichia coli and Saccharomyces cerevisiae
expression of His6-tagged enzyme in Escherichia coli
expression of His6-tagged maltose-binding-protein fusion HPPK in Escherichia coli strain BL21(DE3)
Q7CKD7
expression of the bifunctional 6-hydroxymethyl-7,8-dihydropterin diphosphokinase/dihydropteroate synthase in Escherichia coli
-
gene folK, sequence comparisons, recombinant expression of His6-tagged and maltose-binding-protein-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL
hydroxymethyldihydropterin diphosphokinase from Plasmodium falciparum complements a folK-knockout mutant in Escherichia coli when expressed as a separate polypeptide detached from dihydropteroate synthase. Hydroxymethyldihydropterin diphosphokinase part of the bifunctional protein can function by itself but that a larger part of the polypeptide is needed to ensure full functionality
-
isozyme cytHPPK/DHPS DNA and amino acid sequence determination and analysis, and phylogenetic analysis, transient expression of GFP-tagged isozyme cytHPPK/DHPS in the cytosol of Arabidopsis thaliana protoplasts using transfection via Agrobacterium tumefaciens strain GV3101/pMP90, functional complementation of a Saccharomyces cerevisiae mutant, that lacks the enzyme activity, by expression of isozyme cytHPPK/DHPS, expression and subcloning of N-terminally His6-tagged isozyme cytHPPK/DHPS in Escherichia coli strains DH-5alpha and BL-21AI, co-expression with GroESL chaperones
multifunctional folic acid synthesis fas gene that encodes dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase, in cultured Spodoptera frugiperda SF9 insect cells
-
recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
the hydroxymethyldihydropterin pyrophosphokinase domain of the multifunctional folic acid synthesis Fas protein expressed as an independent enzyme in Escherichia coli, high level expression in inclusion bodies using an inducible tac promoter expression system
-