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2.7.7.1: nicotinamide-nucleotide adenylyltransferase

This is an abbreviated version!
For detailed information about nicotinamide-nucleotide adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.1

Reaction

ATP
+
nicotinamide ribonucleotide
=
diphosphate
 +
NAD+

Synonyms

adenosine triphosphate-nicotinamide mononucleotide transadenylase, adenylyltransferase, nicotinamide mononucleotide, Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase, AtNMNAT, ATP:NMN adenylyltransferase, diphosphopyridine nucleotide pyrophosphorylase, Drosophila NMNAT, GlNMNAT, hNMNAT, hNMNAT-1, hNMNAT-2, human nicotinamide mononucleotide adenylyl-transferase, m-nonN-Nmnat1, mononucleotide adenylyltransferase, More, NAD synthase, NAD+ pyrophosphorylase, nadD, NadM-Nudix, NadR, NaMNAT, nicotinamide 5'-mononucleotide adenylyltransferase, nicotinamide 5'-mononucleotide adenylyltransferase-2, nicotinamide 5-mononucleotide adenylyltransferase-2, nicotinamide adenine dinucleotide pyrophosphorylase, nicotinamide mononucleotide adenylyl-transferase, nicotinamide mononucleotide adenylyltransferase, nicotinamide mononucleotide adenylyltransferase 1, nicotinamide mononucleotide adenylyltransferase 2, nicotinamide mononucleotide adenylyltransferase type 1, nicotinamide mononucleotide adenylyltransferase1, nicotinamide mononucleotide adenylyltransferase2, nicotinamide/nicotinate mononucleotide adenylyltransferase, nicotinamide/nicotinic acid mononucleotide adenylyltransferase, nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, nicotinate/nicotinamide mononucleotide adenyltransferase, NMN adenylyl transferase 1, NMN adenylyltransferase, NMN adenylyltransferase/ADP-ribose pyrophosphatase, NMN AT, NMN-adenylyltransferase, NMN/NaMN adenylyltransferase, NMN/NaMN adenylyltransferase 2, NMN/NaMN adenylyltransferase 3, NMNAT, NMNAT-2, NMNAT1, NMNAT2, NMNAT3, NMNATase, non-nuclear-localized-Nmnat1, PF3D7_1327600, PNAT, Pof1, pyridine nucleotide adenylyltransferase, Tc00.1047053507047.170, TK0067, TTHA1780, yNMNAT-1, yNMNAT-2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.1 nicotinamide-nucleotide adenylyltransferase

Storage Stability

Storage Stability on EC 2.7.7.1 - nicotinamide-nucleotide adenylyltransferase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, several months
-
-20°C, 0.5% v/v Triton X-100, 50 mM sodium phosphte buffer, pH 6.8, 0.5 M NaCl, 100% activity after 3 months
-
-20°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 20% loss of activity
-20°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 55% loss of activity
-20°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 60% loss of activity
-20°C, in 20 mM HEPES buffer, pH 7.5, about 20% loss of activity within 1 month
-
-20°C, in stroma-free lysate: 30% loss of activity within 2-3 days, in washed erythrocytes: up to 1 month
-
-20°C, several weeks without loss in activity
-
-27°C, several months
-
-80°C, 1 mM DTT, 0.5 mM EDTA, 2 weeks
-
-80°C, NMNAT2 is stable
0-3°C, E. coli enzyme: 31 days stable, yeast enzyme: 22% loss of activity within 31 days
-
4°C, 0.3-0.5 mg/ml protein in 0.1-0.3 M NaCl, 1 mM DTT, several months
-
4°C, 0.5% (v/v) Triton X-100, 50 mM sodium phosphate buffer, pH 6.8, 0.5 M NaCl, 80% activity after 3 months
-
4°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 55% loss of activity
4°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 60% loss of activity
4°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 90% loss of activity
4°C, in 20 mM HEPES buffer, pH 7.5, about 30% loss of activity within 2 weeks
-
4°C, in 50 mM potassium phosphate buffer, pH 7.4, 1 mM DTT, 1 mM KCl, 1 mM MgCl2, 0.5 mM EDTA, 1 mM PMSF, several months
-
4°C, NMNAT1 is stable
high KCl concentrations stabilize during storage
-