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2.7.7.12: UDP-glucose-hexose-1-phosphate uridylyltransferase

This is an abbreviated version!
For detailed information about UDP-glucose-hexose-1-phosphate uridylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.12

Reaction

UDP-alpha-D-glucose
+
alpha-D-galactose 1-phosphate
=
alpha-D-glucose 1-phosphate
 +
UDP-alpha-D-galactose

Synonyms

galactose 1-phosphate uridylyltransferase, galactose-1-phosphate uridyl transferase, galactose-1-phosphate uridyltransferase, galactose-1-phosphate uridylyltransferase, galactose-1-phosphate:uridyltransferase, GALT, GALT enzyme, GalT1, GalT2, hexose 1-phosphate uridyltransferase, hexose 1-phosphate uridylyltransferase, hexose-1-phosphate uridylyltransferase, UDP-Glc-hexose-1-P uridylyltransferase, UDP-glucose-hexose-1-P uridylyltransferase, UDPGlc:Gal-1-P uridylyltransferase, UDPglucose-hexose-1-phosphate uridylyltransferase, UDPglucose:alpha-D-galactose-1-phosphate uridylyltransferase, uridine diphosphoglucose:alpha-D-galactose-1-phosphate uridilyltransferase, uridyl transferase, uridyltransferase, uridylylgalactose-1-P uridylyltransferase, uridylyltransferase, hexose 1-phosphate

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.12 UDP-glucose-hexose-1-phosphate uridylyltransferase

Purification

Purification on EC 2.7.7.12 - UDP-glucose-hexose-1-phosphate uridylyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
213fold to homogeneity
-
4190fold
-
45000fold
-
70600fold from erythrocytes
-
about 1800fold to homogeneity
-
about 2000fold
-
about 500fold
His-tagged recombinant wild-type and mutants F171Y and F171L to near homogeneity
-
over 200fold
-
partial from erythrocyte, several isozymes
-
placental enzyme to homogeneity, coelutes with a 67 kDa glycoprotein
-
recombinant bifunctional fusion protein from strain BL21(DE3), pLysS, pT7ET to near homogeneity, 9fold
-
recombinant enzyme from Escherichia coli strain BL21 (DE3) Codon Plus RIL by heat treatment at 80°C for 10 min, followed by anion exchange and cation exchange chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3) plysS
-
recombinant from overexpressing strain BL21(DE3)
-
recombinant His-tagged wild-type and mutant S135L from yeast, to near homogeneity
-
recombinant His-tagged wild-type and mutants P185A, P185S, P185G, P185E, and P185Q to near homogeneity
-
recombinant His-tagged wild-type, partial 67fold, and mutants from Escherichia coli Bl21(DE3)
-
recombinant mutants H166G and H166A
-
recombinant wild-type and mutants from strain BL21(DE3)pLysS to near homogeneity
recombinant wild-type and mutants Q168R and Q168N
-
tagged mutant heterodimers from yeast
-
to homogeneity
wild-type and mutant H166G
-