2.7.7.15: choline-phosphate cytidylyltransferase

This is an abbreviated version!
For detailed information about choline-phosphate cytidylyltransferase, go to the full flat file.

Reaction

Synonyms

AtCCT1, CCT, CCT-alpha, CCT1, CCT2, CCTalpha, CCTalpha236, CCTbeta, CCTbeta2, CCTbeta3, CDP-choline pyrophosphorylase, CDP-choline synthetase, choline phosphate cytidylyltransferase, choline-phosphate cytidylyltransferase A, choline-phosphate cytidylyltransferase-alpha, cholinephosphate cytidylyltransferase, CpCCT1, cpcT, CTalpha, CTbeta2, CTP-phosphocholine cytidylyltransferase, CTP:cholinephosphate cytidylyltransferase, CTP:phosphocholine cytidylyltransferase, CTP:phosphocholine cytidylyltransferase alpha, CTP:phosphocholine cytidylyltransferase alpha-isoform, CTP:phosphocholine cytidylyltransferase beta2, CTP:phosphocholine cytidylyltransferase beta3, CTP:phosphocholine cytidylyltransferase CCT, CTP:phosphocholine cytidylyltransferase-alpha, CTP:phosphocholine cytidylyltransferase-beta2, CTP:phosphocholine cytidylyltranspherase alpha, CTP:phosphorylcholine cytidylyltransferase, CTP:phosphorylcholine cytidylyltransferase CCT1, CTP:phosphorylcholine cytidylyltransferase CCT2, cytidine diphosphocholine pyrophosphorylase, cytidylyltransferase, cytidylyltransferase, choline phosphate, LmjF.18.1330, More, PCYT1A, PF3D7_1316600, PfCCT, phosphocholine cytidylyltransferase, phosphocholine cytidylyltransferase alpha, phosphorylcholine cytidylyltransferase, phosphorylcholine transferase, phosphorylcholine:CTP cytidylyltransferase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.7 Nucleotidyltransferases

                2.7.7.15 choline-phosphate cytidylyltransferase

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Results	in table
36	Activating Compound
5722	AA Sequence
4	Application
1	CAS Registry Number
38	Cloned(Commentary)
1	Crystallization (Commentary)
164	Disease/ Diagnostics
9	Expression
36	General Information
6	General Stability
69	Inhibitors
4	Ki Value [mM]
106	KM Value [mM]
83	Localization
14	Metals/Ions
29	Molecular Weight [Da]
46	Natural Substrates/ Products (Substrates)
267	Organism
11	Pathway
38	PDB ID
11	pH Optimum
1	pH Range
1	pI Value
19	Posttranslational Modification
79	Protein Variants
33	Purification (Commentary)
4	Reaction
1	Reaction Type
102	Reference
104	Source Tissue
31	Specific Activity [micromol/min/mg]
10	Storage Stability
126	Substrates and Products (Substrate)
24	Subunits
117	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
9	Temperature Stability [°C]
14	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.7.7.15 - choline-phosphate cytidylyltransferase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C stable for several months in 50% glycerol/20 mM Tris pH 7.5

Streptococcus pneumoniae

-

642922

-20°C, enzyme concentrated to a small volume, 50% glycerol, 20% loss of activity after 2 weeks

Ricinus communis

-

642901

-20°C, stable for at least 8 months in 70% ammonium sulfate

Streptococcus pneumoniae

Q93MI4

642912

-20°C, stable for more than 1 month in presence of 20% glycerol

Ricinus communis

-

642899

-70°C, 50 mM Tris-HCl, 150 mM NaCl, 1.0 mM EDTA, 2.0 mM DTT, 0.025% NaN3, pH 7.4, 0.03% Triton X-100, 200 mM phosphate, stable for several months

Rattus norvegicus

-

642889

-70°C, stable in presence of Triton X-100 and 0.2 M potassium phosphate

Rattus norvegicus

-

642894

0°C, 80% loss of activity after 6 days

Rattus norvegicus

-

642900

4°C, 20% loss of activity per day

Rattus norvegicus

-

642889

4°C, 30% loss of activity after 1 week

Ricinus communis

-

642899

4°C, increase of activity in fresh cytosol obtained by storage is due to an increase of lysophosphatidylethanolamine and a decrease in phosphatidylethanolamine

Rattus norvegicus

-

642898