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5.8
D-glucose 1-phosphate
-
pH 7.5, 37°C, 5 mM UTP
0.38 - 1.3
N-acetyl-alpha-D-galactosamine 1-phosphate
0.008 - 0.861
N-acetyl-alpha-D-glucosamine 1-phosphate
0.011 - 2.25
N-acetyl-D-glucosamine 1-phosphate
6.3
UDP-D-glucose
-
pH 8.5, 37°C, 5 mM diphosphate
0.016
UDP-N-acetyl-alpha-D-glucosamine
pH 7.5, 80°C
0.808 - 2.768
UDP-N-acetyl-D-galactosamine
0.065 - 6.1
UDP-N-acetyl-D-glucosamine
additional information
additional information
-
0.016
diphosphate
pH 7.5, 80°C
0.79
diphosphate
-
pH 7.4, 37°C
5
diphosphate
-
pH 7.5, 30°C
5.4
diphosphate
-
pH 7.5, 30°C
0.38
N-acetyl-alpha-D-galactosamine 1-phosphate
-
AGX1, 37°C, pH 7.5
1.1
N-acetyl-alpha-D-galactosamine 1-phosphate
-
pH 7.5, 37°C, 5 mM UTP
1.3
N-acetyl-alpha-D-galactosamine 1-phosphate
-
AGX2, 37°C, pH 7.5
0.008
N-acetyl-alpha-D-glucosamine 1-phosphate
pH 7.5, 80°C
0.03
N-acetyl-alpha-D-glucosamine 1-phosphate
pH 7.5, 30°C
0.03
N-acetyl-alpha-D-glucosamine 1-phosphate
purified recombinant native enzyme, pH 7.5, 37°C
0.041
N-acetyl-alpha-D-glucosamine 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.061
N-acetyl-alpha-D-glucosamine 1-phosphate
-
pH 8.2, 37°C, recombinant His6-tagged enzyme
0.068
N-acetyl-alpha-D-glucosamine 1-phosphate
wild-type enzyme, pH 7.5, 80°C
0.068
N-acetyl-alpha-D-glucosamine 1-phosphate
-
wild-type enzyme, pH 7.5, 80°C
0.068
N-acetyl-alpha-D-glucosamine 1-phosphate
wild-type ST0452, pH 7.5, 80°C
0.097
N-acetyl-alpha-D-glucosamine 1-phosphate
mutant enzyme Y97M, pH 7.5, 80°C
0.097
N-acetyl-alpha-D-glucosamine 1-phosphate
-
mutant enzyme Y97M, pH 7.5, 80°C
0.097
N-acetyl-alpha-D-glucosamine 1-phosphate
mutant Y97M, pH 7.5, 80°C
0.147
N-acetyl-alpha-D-glucosamine 1-phosphate
mutant enzyme Y97N, pH 7.5, 80°C
0.147
N-acetyl-alpha-D-glucosamine 1-phosphate
-
mutant enzyme Y97N, pH 7.5, 80°C
0.147
N-acetyl-alpha-D-glucosamine 1-phosphate
mutant Y97N, pH 7.5, 80°C
0.861
N-acetyl-alpha-D-glucosamine 1-phosphate
mutant enzyme Y97V, pH 7.5, 80°C
0.861
N-acetyl-alpha-D-glucosamine 1-phosphate
-
mutant enzyme Y97V, pH 7.5, 80°C
0.861
N-acetyl-alpha-D-glucosamine 1-phosphate
mutant Y97V, pH 7.5, 80°C
0.011
N-acetyl-D-glucosamine 1-phosphate
R116A mutant, 30°C, pH 8.3
0.012
N-acetyl-D-glucosamine 1-phosphate
P122A mutant, 30°C, pH 8.3
0.014
N-acetyl-D-glucosamine 1-phosphate
wild type enzyme, 30°C, pH 8.3
0.015
N-acetyl-D-glucosamine 1-phosphate
T115A mutant, 30°C, pH 8.3
0.018
N-acetyl-D-glucosamine 1-phosphate
K123A mutant, 30°C, pH 8.3
0.019
N-acetyl-D-glucosamine 1-phosphate
G114A mutant, 30°C, pH 8.3
0.029
N-acetyl-D-glucosamine 1-phosphate
L117A mutant, 30°C, pH 8.3
0.034
N-acetyl-D-glucosamine 1-phosphate
G111A mutant, 30°C, pH 8.3
0.044
N-acetyl-D-glucosamine 1-phosphate
pH 7.5, 37°C
0.07
N-acetyl-D-glucosamine 1-phosphate
-
pH 8.2, 37°C
0.124
N-acetyl-D-glucosamine 1-phosphate
G112A mutant, 30°C, pH 8.3
0.18
N-acetyl-D-glucosamine 1-phosphate
pH 7.6, 37°C
0.29
N-acetyl-D-glucosamine 1-phosphate
-
pH 7.5, 37°C, 5 mM UTP
0.337
N-acetyl-D-glucosamine 1-phosphate
pH 7.6, 37°C
2.25
N-acetyl-D-glucosamine 1-phosphate
-
30°C
0.808
UDP-N-acetyl-D-galactosamine
pH 7.6, 37°C
2.2
UDP-N-acetyl-D-galactosamine
-
pH 8.5, 37°C, 5 mM diphosphate
2.768
UDP-N-acetyl-D-galactosamine
pH 7.6, 37°C
0.065
UDP-N-acetyl-D-glucosamine
pH 7.6, 37°C
0.219
UDP-N-acetyl-D-glucosamine
pH 7.6, 37°C
0.23
UDP-N-acetyl-D-glucosamine
-
pH 8.5, 37°C, 5 mM diphosphate
0.36
UDP-N-acetyl-D-glucosamine
-
pH 7.4, 37°C
0.4
UDP-N-acetyl-D-glucosamine
-
30°C
0.7
UDP-N-acetyl-D-glucosamine
-
AGX1, pH 8.5, 37°C
0.8
UDP-N-acetyl-D-glucosamine
-
P220A, Y227A, R115A mutants, pH 8.5, 37°C
2.2
UDP-N-acetyl-D-glucosamine
-
pH 7.5, 30°C
2.2
UDP-N-acetyl-D-glucosamine
-
G224A mutants, pH 8.5, 37°C
6.1
UDP-N-acetyl-D-glucosamine
-
pH 7.5, 30°C
0.0017
UTP
pH 7.5, 80°C
0.03
UTP
pH 7.5, 37°C, recombinant enzyme
0.038
UTP
Mg-UTP, purified recombinant native enzyme, pH 7.5, 37°C
0.095
UTP
-
pH 8.2, 37°C, recombinant His6-tagged enzyme
0.42
UTP
-
pH 7.5, 37°C, 10 mM N-acetyl-D-glucose 1-phosphate
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Km values are 3.54 microM/l, 13.9 microM/l, 4.22 microM/l, 13.3 microM/l for isoforms MP I, MP II, MP III, MP IV, respcetively
-
additional information
additional information
-
the UAP enzyme kinetics display a strictly ordered bi.bi mechanism
-
additional information
additional information
kinetics of wild-type and mutant enzymes for Glc-1-P UTase activities, overview
-
additional information
additional information
-
kinetics of wild-type and mutant enzymes for Glc-1-P UTase activities, overview
-