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2.7.7.27: glucose-1-phosphate adenylyltransferase

This is an abbreviated version!
For detailed information about glucose-1-phosphate adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.27

Reaction

ATP
+
alpha-D-glucose 1-phosphate
=
diphosphate
 +
ADP-alpha-D-glucose

Synonyms

adenosine 5'-diphosphate (ADP)-glucose pyrophosphorylase, adenosine 5'-diphosphate glucose pyrophosphorylase, adenosine diphosphate glucose pyrophosphorylase, adenosine diphosphoglucose pyrophosphorylase, adenosine-5'-diphosphoglucose pyrophosphorylase, adenylyltransferase, glucose 1-phosphate, ADG2, ADP glucose pyrophosphorylase, ADP-Glc PPase, ADP-Glc pyrophosphorylase, ADP-glucose pyrophosphorylase, ADP-glucose synthase, ADP-glucose synthetase, ADPG pyrophosphorylase, ADPGlc PPase, ADPglucose pyrophosphorylase, AGP, AGP-S, AGP1, AGP2, AGPase, AGPL2, AGPS, AGPS1, AGPS2b, APS1, Brittle-2, BT2, GlgC, GlgD, OtaL, OtaS, SH2, Shrunken-2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.27 glucose-1-phosphate adenylyltransferase

General Stability

General Stability on EC 2.7.7.27 - glucose-1-phosphate adenylyltransferase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
bovine serum albumin stabilizes the purified enzyme at 0.5 mg/ml
endosperm enzyme stability is increased by ammonium sulfate, as a supernatant of 30% saturing retains 50% of the activity after 5 min heating at 60°C, by additionally polyethylenegycol retains 70% of enzyme activity
freeze-thawing reduces activity by 70%, high ionic strength, ATP and Mg2+ stabilize
-
irreversibly insoluble when dialyzed against deionized water
-
no reducing reagent required during purification
-
phosphate stabilizes
-
phosphate, in the absence of 3-PGA, stabilizes endosperm AGPase
-
PMSF and chymostatin prevent proteolysis during purification, not p-aminobenzamidine, N-alpha-p-tosyl-L-lysine chloromethylketone, NEM, PCMB, benzamidine, leupeptin, pepstatin or EDTA
-
polyvinylpyrrolidone, i.e. PVP fractionation stabilizes during purification, 20% sucrose and 30 mM phosphate stabilize during purification
-
the irradiation of enzyme in the presence of 8-N3ATP, fructose 1,6-phosphate and Mg2+ result in the covalent modification accompanying the loss of the enzyme activity
-