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0.25
8-N3ADPglucose
-
pH 8.5, 37°C
1.25
8-N3ATP
-
pH 8.5, 37°C
0.007 - 1.2
ADP-alpha-D-glucose
0.00038 - 0.9
diphosphate
0.047 - 0.12
phosphoenolpyruvate
additional information
additional information
-
0.104
ADP
-
pH 8.0, 37°C, in absence of phosphoenolpyruvate
0.235
ADP
-
pH 8.0, 37°C, strain ATCC 15365
0.41
ADP
-
influence of activators
0.42
ADP
-
pH 8.0, 37°C, strains JP102 and LT-2, in presence of 0.25 mM fructose 1,6-bisphosphate
1
ADP
-
pH 8.0, 37°C, strains JP102, in presence of 1 mM fructose 1,6-bisphosphate
1.1
ADP
-
pH 8.0, 37°C, strains LT-2, in presence of 1 mM fructose 1,6-bisphosphate
1.2
ADP
-
pH 7.5, 37°C, in absence of 3-phosphoglycerate
1.7
ADP
-
pH 8.0, 37°C, in presence of fructose 1,6-bisphosphate
2
ADP
-
pH 7.5, 37°C, in presence of 3-phosphoglycerate
2
ADP
-
pH 8.0, 37°C, in presence of fructose 6-phosphate
3.8
ADP
-
in absence of fructose 6-phosphate
3.8
ADP
-
in absence of fructose 6-phosphate
5
ADP
-
in presence of 0.5 mM fructose 6-phosphate
5
ADP
-
in presence of 0.5 mM fructose 6-phosphate
0.007
ADP-alpha-D-glucose
wild-type, substrate ATP, pH 7.4, 37°C
0.01
ADP-alpha-D-glucose
chimeric mutant, substrate ATP, pH 7.4, 37°C
0.045
ADP-alpha-D-glucose
-
oxidized wild-type, substrate ATP, pH 7.4, 37°C
0.05
ADP-alpha-D-glucose
-
chimeric mutant, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.05
ADP-alpha-D-glucose
chimeric mutant, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.083
ADP-alpha-D-glucose
-
chimeric mutant, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.083
ADP-alpha-D-glucose
chimeric mutant, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.09
ADP-alpha-D-glucose
-
chimeric mutant, substrate glucose 1-phosphate, pH 7.4, 37°C
0.09
ADP-alpha-D-glucose
chimeric mutant, substrate glucose 1-phosphate, pH 7.4, 37°C
0.09
ADP-alpha-D-glucose
wild-type, substrate glucose 1-phosphate, pH 7.4, 37°C
0.11
ADP-alpha-D-glucose
-
oxidized wild-type, substrate glucose 1-phosphate, pH 7.4, 37°C
0.11
ADP-alpha-D-glucose
-
oxidized wild-type, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.12
ADP-alpha-D-glucose
wild-type, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.13
ADP-alpha-D-glucose
-
oxidized wild-type, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.14
ADP-alpha-D-glucose
-
reduced wild-type, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.19
ADP-alpha-D-glucose
-
chimeric mutant, substrate ATP, pH 7.4, 37°C
0.21
ADP-alpha-D-glucose
-
reduced wild-type, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.46
ADP-alpha-D-glucose
-
reduced wild-type, substrate glucose 1-phosphate, pH 7.4, 37°C
1.2
ADP-alpha-D-glucose
wild-type, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.007
AMP
-
pH 8.0, 37°C, wild-type enzyme in presence of 0.1 mM fructose-1,6-bisphosphate
0.012
AMP
-
pH 8.0, 37°C, in absence of phosphoenolpyruvate
0.015
AMP
-
pH 8.0, 37°C, strain ATCC 15365
0.023
AMP
-
pH 8.0, 37°C, in presence of phosphoenolpyruvate
0.028
AMP
-
pH 8.0, 37°C, strain LT-2, in presence of 0.25 mM fructose 1,6-bisphosphate
0.031
AMP
-
pH 8.0, 37°C, strain ATCC 274
0.032
AMP
-
pH 8.0, 37°C, strain JP102, in presence of 0.25 mM fructose 1,6-bisphosphate
0.04
AMP
-
pH 7.6, 37°C, wild-type enzyme
0.06
AMP
-
pH 7.6, 37°C, mutant enzyme D142E
0.062
AMP
-
in absence of pyruvate
0.094
AMP
-
pH 7.0, 37°C, wild-type enzyme, in the presence of a saturating concentration of fructose 1,6-bisphosphate
0.096
AMP
-
pH 8.0, 37°C, strain JP102, in presence of 1 mM fructose 1,6-bisphosphate
0.11
AMP
-
pH 8.0, 37°C, strain LT-2, in presence of 1 mM fructose 1,6-bisphosphate
0.13
AMP
-
pH 7.6, 37°C, mutant enzyme D142A
0.204
AMP
-
pH 8.0, 37°C, in presence of phosphoenolpyruvate
0.26
AMP
-
in presence of 0.05 mM pyruvate
0.3
AMP
-
pH 7.0, 37°C, mutant enzyme P295G, in the presence of a saturating concentration of fructose 1,6-bisphosphate,
0.34
AMP
-
pH 7.0, 37°C, mutant enzyme P295N, in the presence of a saturating concentration of fructose 1,6-bisphosphate
0.38
AMP
-
pH 7.0, 37°C, mutant enzyme P295Q, in the presence of a saturating concentration of fructose 1,6-bisphosphate
0.64
AMP
-
influence of activators
0.95
AMP
-
pH 7.0, 37°C, mutant enzyme G336D, in the presence of a saturating concentration of fructose 1,6-bisphosphate
0.96
AMP
-
pH 7.0, 37°C, mutant enzyme P295E, in the presence of a saturating concentration of fructose 1,6-bisphosphate
1
AMP
-
pH 7.6, 37°C, mutant enzyme D142N
2
AMP
-
pH 7.0, 37°C, mutant enzyme P295D, in the presence of a saturating concentration of fructose 1,6-bisphosphate
2.5
AMP
-
in presence of 1 mM pyruvate
3
AMP
-
pH 8.0, 37°C, wild-type enzyme in presence of 0.3 mM fructose-1,6-bisphosphate
4.95
AMP
-
pH 7.0, 37°C, double mutant enzyme P295D/G336D
9
AMP
-
pH 8.0, 37°C, the chimeric enzymes, AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme is slightly activated by AMP in the range of 0.1-2 mM
9
AMP
-
pH 8.0, 37°C, the chimeric enzymes, AE contains the N-terminus of Agrobacterium tumefaciens enzyme and the C-terminus of Escherichia coli enzyme is slightly activated by AMP in the range of 0.1-2 mM
0.00038
diphosphate
-
pH 7.4, 37°C
0.025
diphosphate
-
reduced wild-type, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.03
diphosphate
chimeric mutant, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.047
diphosphate
-
oxidized wild-type, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.047
diphosphate
-
oxidized wild-type, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.047
diphosphate
-
reduced wild-type, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.075
diphosphate
chimeric mutant, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.1
diphosphate
wild-type, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.11
diphosphate
wild-type, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.19
diphosphate
-
oxidized wild-type, substrate glucose 1-phosphate, pH 7.4, 37°C
0.19
diphosphate
wild-type, substrate glucose 1-phosphate, pH 7.4, 37°C
0.35
diphosphate
-
chimeric mutant, substrate glucose 1-phosphate, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.35
diphosphate
-
reduced wild-type, substrate glucose 1-phosphate, pH 7.4, 37°C
0.36
diphosphate
-
chimeric mutant, substrate ATP, pH 7.4, 37°C
0.36
diphosphate
chimeric mutant, substrate ATP, pH 7.4, 37°C
0.44
diphosphate
-
chimeric mutant, substrate glucose 1-phosphate, pH 7.4, 37°C
0.44
diphosphate
chimeric mutant, substrate glucose 1-phosphate, pH 7.4, 37°C
0.55
diphosphate
-
chimeric mutant, substrate ATP, presence of 3-phosphoglycerate, pH 7.4, 37°C
0.55
diphosphate
-
reduced wild-type, substrate ATP, pH 7.4, 37°C
0.81
diphosphate
-
oxidized wild-type, substrate ATP, pH 7.4, 37°C
0.9
diphosphate
wild-type, substrate ATP, pH 7.4, 37°C
0.022
phosphate
-
pH 7.5, in absence of activators
0.025
phosphate
pH 8.0, in absence of 0.25 mM 3-phosphoglycerate
0.038
phosphate
-
pH 8.5, 37°C, in absence of 3-phosphoglycerate
0.04
phosphate
-
pH 8.0, 37°C, recombinant heterotetrameric enzyme, in absence of 3-phosphoglycerate
0.044
phosphate
-
pH 7.0, 37°C, in absence of 3-phosphoglycerate
0.045
phosphate
-
pH 8.0, 37°C, in absence of 3-phosphoglycerate
0.054
phosphate
-
pH 8.0, 37°C, in absence of 3-phosphoglycerate
0.06
phosphate
-
pH 7.5, 37°C, in absence of 3-phosphoglycerate
0.064
phosphate
-
pH 7.3, 37°C, in absence of phosphoglycerate
0.072
phosphate
-
in absence of 3-phosphoglycerate
0.08
phosphate
-
pH 8.0, 37°C, the small subunit of enzyme, in presence of 3 mM 3-phosphoglycerate
0.088
phosphate
-
pH 7.5, 37°C, in absence of 3-phosphoglycerate
0.095
phosphate
-
pH 7.0, 37°C, in absence of 3-phosphoglycerate
0.12
phosphate
-
pH 7.5, 37°C, in presence of 3-phosphoglycerate
0.14
phosphate
-
pH 7.0, 37°C, in absence of 3-phosphoglycerate
0.18
phosphate
-
pH 8.5, in absence of 3-phosphoglycerate
0.18
phosphate
-
pH 7.5, 30°C, in the absence of 3-phosphoglycerate
0.19
phosphate
-
pH 8.0, in presence of 0.5 mM 3-phosphoglycerate
0.2
phosphate
-
pH 8.0, 37°C, in presence of 0.25 mM 3-phosphoglycerate
0.2
phosphate
leaf enzyme in absence of 3-phosphoglycerate
0.23
phosphate
-
in absence of 0.01 mM fructose 6-phosphate
0.26
phosphate
-
influence of activators
0.37
phosphate
pH 8.0, 37°C
0.4
phosphate
-
pH 8.0, in presence of 1 mM 3-phosphoglycerate
0.44
phosphate
-
pH 7.4, 37°C, in presence of 1 mM 3-phosphoglycerate
0.46
phosphate
-
pH 7.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate
0.53
phosphate
-
pH 8.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate
0.57
phosphate
-
pH 7.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate
0.63
phosphate
-
pH 8.0, 37°C, recombinant heterotetrameric enzyme, in presence of 3 mM 3-phosphoglycerate
0.7
phosphate
-
in absence of fructose 6-phosphate
0.7
phosphate
endosperm enzyme in absence of 3-phosphoglycerate
0.72
phosphate
-
pH 8.5, 37°C, in presence of 3-phosphoglycerate
0.91
phosphate
endosperm enzyme in presence of 0.25 mM 3-phosphoglycerate
0.93
phosphate
-
pH 8.0, 37°C, strains LT-2, in presence of 0.25 mM fructose 1,6-bisphosphate
0.97
phosphate
-
pH 7.3, 37°C, in presence of 3-phosphoglycerate
1
phosphate
-
pH 8.5, in presence of 2 mM 3-phosphoglycerate
1
phosphate
-
the phosphate inhibition pattern of Mos(1-198) is complex and biphasic. Inhibition at relatively high phopshate concentrations is less than predicted at low phosphate concentrations, the calculated Ki for phosphate is about 1 mM before 50% inhibition and 36 mM after 50% inhibition. Inhibition kinetics of mutants in presence and absence of 3-phosphoglycerate
1
phosphate
-
the phosphate inhibition pattern of Mos(1-198) is complex and biphasic. Inhibition at relatively high phosphate concentrations is less than predicted at low phosphate concentrations, the calculated Ki for phosphate is about1 mM before 50% inhibition and 36 mM after 50% inhibition. Inhibition kinetics of mutants in presence and absence of 3-phosphoglycerate
1.15
phosphate
-
pH 7.5, 37°C, in presence of 3-phosphoglycerate
1.27
phosphate
-
pH 7.3, 37°C, in presence of 3-phosphoglycerate
1.3
phosphate
-
pH 8.0, 37°C, strains JP102, in presence of 0.25 mM fructose 1,6-bisphosphate
1.3
phosphate
-
pH 7.5, in presence of 1 mM 3-phosphoglycerate
1.4
phosphate
-
wild-type enzyme, in presence of 3-phosphoglycerate
1.4
phosphate
-
wild-type enzyme, in presence of 3-phosphoglycerate
1.5
phosphate
-
pH 7.0, 37°C, in presence of 3-phosphoglycerate
1.5
phosphate
-
pH 7.5, 5°C, in the absence of 3-phosphoglycerate
1.52
phosphate
endosperm enzyme in presence of 1 mM 3-phosphoglycerate
1.7
phosphate
-
pH 8.0, 37°C, strains LT-2, in presence of 1 mM fructose 1,6-bisphosphate
1.83
phosphate
mutant BT2/S163F, 15 mM 3-phosphoglycerate
2
phosphate
-
pH 8.0, 37°C, strains JP102, in presence of 1 mM fructose 1,6-bisphosphate
2.28
phosphate
mutant BT2/C382F, 15 mM 3-phosphoglycerate
2.4
phosphate
-
in absence of 0.1 mM fructose 6-phosphate
2.49
phosphate
endosperm enzyme in presence of 5 mM 3-phosphoglycerate
2.96
phosphate
-
pH 7.4, 37°C, enzyme from maize endosperm
3.21
phosphate
mutant BT2/Q213H, 15 mM 3-phosphoglycerate
3.72
phosphate
mutant BT2/P372A, 15 mM 3-phosphoglycerate
3.95
phosphate
-
pH 7.4, 37°C, short day photoperiod
3.96
phosphate
mutant BT2/H149S, 15 mM 3-phosphoglycerate
4.26
phosphate
mutant BT2/D368S, 15 mM 3-phosphoglycerate
4.26
phosphate
-
pH 7.4, 37°C, long day photoperiod
5.34
phosphate
mutant BT2/T361C, 15 mM 3-phosphoglycerate
8.7
phosphate
-
pH 8.0, 37°C, strain ATCC 15365
8.7
phosphate
-
mutant Mos(1-198), in presence of 3-phosphoglycerate
8.7
phosphate
-
mutant Mos(1-198), in presence of 3-phosphoglycerate
9.8
phosphate
-
pH 7.4, 37°C, in presence of 10 mM 3-phosphoglycerate
13.23
phosphate
mutant BT2/C114A, 15 mM 3-phosphoglycerate
14.5
phosphate
mutant BT2/V227R, 15 mM 3-phosphoglycerate
15.22
phosphate
mutant BT2/E438Q, 15 mM 3-phosphoglycerate
16.8
phosphate
wild type BT2/SH2, 15 mM 3-phosphoglycerate
17.61
phosphate
mutant BT2/M172T, 15 mM 3-phosphoglycerate
17.93
phosphate
mutant BT2/V502T, 15 mM 3-phosphoglycerate
18.36
phosphate
mutant BT2/C424V, 15 mM 3-phosphoglycerate
20.43
phosphate
mutant BT2/A508S, 15 mM 3-phosphoglycerate
0.047
phosphoenolpyruvate
-
influence of activators
0.12
phosphoenolpyruvate
pH 8.0, 37°C
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
Ki of AMP, effect of fructose 1,6-bisphosphate on wild-type and mutant enzymes
-
additional information
additional information
-
Ki of phosphate, ADP, AMP when NADPH, fructose 1,6-bisphosphate and pyridoxalphosphate are activators
-
additional information
additional information
-
Ki of phosphate, ADP, AMP when NADPH, fructose 1,6-bisphosphate and pyridoxalphosphate are activators
-
additional information
additional information
comparison of Ki of phosphate
-
additional information
additional information
-
comparison of Ki of phosphate
-