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2.7.7.27: glucose-1-phosphate adenylyltransferase

This is an abbreviated version!
For detailed information about glucose-1-phosphate adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.27

Reaction

ATP
+
alpha-D-glucose 1-phosphate
=
diphosphate
 +
ADP-alpha-D-glucose

Synonyms

adenosine 5'-diphosphate (ADP)-glucose pyrophosphorylase, adenosine 5'-diphosphate glucose pyrophosphorylase, adenosine diphosphate glucose pyrophosphorylase, adenosine diphosphoglucose pyrophosphorylase, adenosine-5'-diphosphoglucose pyrophosphorylase, adenylyltransferase, glucose 1-phosphate, ADG2, ADP glucose pyrophosphorylase, ADP-Glc PPase, ADP-Glc pyrophosphorylase, ADP-glucose pyrophosphorylase, ADP-glucose synthase, ADP-glucose synthetase, ADPG pyrophosphorylase, ADPGlc PPase, ADPglucose pyrophosphorylase, AGP, AGP-S, AGP1, AGP2, AGPase, AGPL2, AGPS, AGPS1, AGPS2b, APS1, Brittle-2, BT2, GlgC, GlgD, OtaL, OtaS, SH2, Shrunken-2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.27 glucose-1-phosphate adenylyltransferase

Specific Activity

Specific Activity on EC 2.7.7.27 - glucose-1-phosphate adenylyltransferase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0021
wild-type APS1, forward reaction
0.01
mutant D143N, small subunit
0.013
-
in crude extracts, study of influence of fructose 1,6-bisphosphate and fructose 6-phosphate
0.014
0.02
mutation K41R, large subunit, plus D143N, small subunit
0.03
-
wild type
0.066
0.09
-
transgenic line CS8-13
0.1
-
transgenic line CS8-29
0.125
-
strain ATCC 15365
0.17
-
transgenic line CS8-3
0.19
-
transgenic line CS8-18
0.287
-
strain ATCC 274
0.5
-
mutant lacking N-terminal 19 amino acids, 37°C, pH 8.0
0.61
-
mutant W113A, presence of 0.5 mM AMP plus 2.5 mM fructose 1,6-bisphosphate , pH 8.0, 37°C
0.63
-
mutant W113A, presence of 0.5 mM AMP, pH 8.0, 37°C
0.82
-
mutant Q74A, presence of 0.5 mM AMP plus 2.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C
1.02
mutation T51K, large subunit, plus D143N, small subunit
1.05
-
mutant Q74A, presence of 0.5 mM AMP, pH 8.0, 37°C
1.08
-
wild-type, pH 8.0, 37°C
1.12
-
mutant W113A, presence of 2.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C
1.43
-
-
1.45
-
mutant W113A, pH 8.0, 37°C
1.93
-
mutant Q74A, pH 8.0, 37°C
10.87
100
-
wild-type enzyme
105
-
mutant lacking N-terminal 15 amino acids, 37°C, pH 8.0
11.6
plastidial enzyme
110
-
double mutant enzymes
12.3
cytosolic enzyme
129
-
wild-type enzyme
13.1
large subunit mutant D161G, 37°C, pH 7.4
13.4
mutant D157E
131
-
wild-type, 37°C, pH 8.0
14.2
mutant T129V
148
-
wild-type enzyme
15.2
wild-type, 37°C, pH 7.4
16
recombinant enzyme, homotetramer APS1
17
recombinant enzyme APS1 plus APL4
19.1
mutant D157N
2.4
endosperm enzyme
2.9
mutation K41R/T51K, large subunit, plus D143N, small subunit
21.81
23
mutant K41R/T51K
23.7
mutant T51K
26.8
mutant G267S
3.2
mutant G128L
3.6
-
wild-type, presence of 0.5 mM AMP plus 2.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C
3.7
recombinant enzyme APS2 plus APL1
34
-
-
37.5
mutant K41R
39.8
mutant Q127M
4.4
-
-
40.5
mutant G36A
41.1
-
recombinant enzyme from maize endosperm
47.6
-
wild-type, presence of 2.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C
5
recombinant enzyme APS1 plus APL2
5.4
mutant A132V
5.6
mutant A132D
58.5
leaf enzyme
6.5
mutant G267L
6.7
mutant A132N
7.6
mutant G128A
7.8
large subunit mutant D161G, 55°C, pH 7.4
80 - 100
-
mutant enzymes
86
-
coexpression of mutant lacking N-terminal 15 amino acids and C-terminal 108 amino acids with C-terminal peptide of 108 amino acids, 37°C, pH 8.0
9.5
mutant G37A
90 - 110
-
-
94
-
mutant lacking N-terminal 3 amino acids, 37°C, pH 8.0
98
-
mutant lacking N-terminal 11 amino acids, 37°C, pH 8.0
additional information