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2.7.7.27: glucose-1-phosphate adenylyltransferase

This is an abbreviated version!
For detailed information about glucose-1-phosphate adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.27

Reaction

ATP
+
alpha-D-glucose 1-phosphate
=
diphosphate
 +
ADP-alpha-D-glucose

Synonyms

adenosine 5'-diphosphate (ADP)-glucose pyrophosphorylase, adenosine 5'-diphosphate glucose pyrophosphorylase, adenosine diphosphate glucose pyrophosphorylase, adenosine diphosphoglucose pyrophosphorylase, adenosine-5'-diphosphoglucose pyrophosphorylase, adenylyltransferase, glucose 1-phosphate, ADG2, ADP glucose pyrophosphorylase, ADP-Glc PPase, ADP-Glc pyrophosphorylase, ADP-glucose pyrophosphorylase, ADP-glucose synthase, ADP-glucose synthetase, ADPG pyrophosphorylase, ADPGlc PPase, ADPglucose pyrophosphorylase, AGP, AGP-S, AGP1, AGP2, AGPase, AGPL2, AGPS, AGPS1, AGPS2b, APS1, Brittle-2, BT2, GlgC, GlgD, OtaL, OtaS, SH2, Shrunken-2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.27 glucose-1-phosphate adenylyltransferase

Storage Stability

Storage Stability on EC 2.7.7.27 - glucose-1-phosphate adenylyltransferase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, in 50 mM HEPES buffer, pH 8.0, 10% sucrose, 5mM MgCl2, 0.1 mM EDTA, stable for at least 3 months
-20°C, in 60% (NH4)2SO4, 15 weeks
-
-20°C, purified endosperm enzyme, 20% loss of activity after 1 month
-20°C, purified leaf enzyme, stable for at least 3 months
-70°C, 50 mM Tris-HCl, pH 7.2, 2.5 mM dithioerythritol, at least 1 year
-
-70°C, in 50 mM Tris-HCl buffer, pH 7.2, 10 mM potassium phosphate, 0.5 mM dithioerythritol, 20% glycerol, stable
-
-80°C, 50 mM HEPES pH 8.0, 0.5 mM dithiothreitol, 0.1 mM EDTA, 20% w/v sucrose, stable for 12 months
-80°C, majority of activity is retained for several months, mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber
-
-80°C, majority of activity is retained for several months, recombinant enzyme from maize and mosaic AGPases derived from protein motifs normally expressed in the Zea mays endosperm and the Solanum tuberosum tuber
-
-80°C, stable for at least 3 months
-80°C, stable for more than 3 months
0°C, in 30 mM potassium phosphate buffer, pH 7, 4 days
-
0°C, partially purified, at least several weeks
-
4°C, 30-50% loss of activity overnight
-
4°C, endosperm extract, 6-8 h, 50% loss of activity
4°C, in 50 mM HEPES buffer, pH 7, 20% sucrose, 0.2 mM dithioerythritol, 1 mM EDTA, 2 weeks, about 60% loss of activity within 1 month
-
4°C, in 50 mM HEPES buffer, pH 7.5, 20% sucrose, 1 mM EDTA, 2 mM GSH, at least 6 weeks
-
frozen or 4°C, most stable in buffer containing 20% sucrose and 30 mM phosphate
-
frozen, partially purified preparation, up to 2 weeks
-