2.7.7.31: DNA nucleotidylexotransferase
This is an abbreviated version!
For detailed information about DNA nucleotidylexotransferase, go to the full flat file.
Word Map on EC 2.7.7.31
-
2.7.7.31
-
dutp
-
nick
-
tunel
-
nick-end
-
caspase-3
-
leukemia
-
tdt-mediated
-
necrosis
-
bcl-2
-
marrow
-
tunel-positive
-
lymphoblast
-
lymphocyte
-
neuroprotective
-
sham
-
lymphoid
-
ischemia
-
eosin
-
infarct
-
b-cell
-
hematoxylin
-
end-labeling
-
deoxyuridine
-
cerebral
-
thymus
-
polymerases
-
malondialdehyde
-
reperfusion
-
thymocytes
-
ladder
-
cardiomyocytes
-
anti-apoptotic
-
ischemia-reperfusion
-
immunophenotype
-
myeloid
-
immunoglobulin
-
dutp-biotin
-
occlusion
-
nissl
-
labeling-positive
-
analysis
-
french-american-british
-
3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium
-
blastic
-
internucleosomal
-
t-lymphoblastic
-
apoptosis-related
-
transcriptases
-
2'-deoxyuridine
-
biotechnology
-
klenow
-
calla
-
medicine
- 2.7.7.31
- dutp
- nick
-
tunel
-
nick-end
- caspase-3
- leukemia
-
tdt-mediated
- necrosis
- bcl-2
- marrow
-
tunel-positive
- lymphoblast
- lymphocyte
-
neuroprotective
-
sham
-
lymphoid
- ischemia
- eosin
- infarct
- b-cell
- hematoxylin
-
end-labeling
- deoxyuridine
- cerebral
- thymus
- polymerases
- malondialdehyde
-
reperfusion
- thymocytes
-
ladder
-
cardiomyocytes
-
anti-apoptotic
-
ischemia-reperfusion
-
immunophenotype
- myeloid
- immunoglobulin
-
dutp-biotin
- occlusion
-
nissl
-
labeling-positive
- analysis
-
french-american-british
-
3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium
-
blastic
-
internucleosomal
-
t-lymphoblastic
-
apoptosis-related
- transcriptases
- 2'-deoxyuridine
- biotechnology
-
klenow
- calla
- medicine
Reaction
Synonyms
addase, bTdT, deoxynucleotidyl terminal transferase, deoxynucleotidyl transferase, deoxyribonucleic acid nucleotidyltransferase, deoxyribonucleic nucleotidyltransferase, DNA-polymerizing enzyme, DNTT, hTdT, hTdTL1, hTdTL2, hTdTS, mTdT, nucleotidyltransferase, terminal deoxyribo-, Rep75, TdT, terminal addition enzyme, terminal deoxynucleotide transferase, terminal deoxynucleotidyl transferase, terminal deoxynucleotidyl transferase (TDT), terminal deoxynucleotidyltransferase, terminal deoxynucleotiyl transferase, terminal deoxyribonucleotidyl transferase, terminal deoxyribonucleotidyltransferase
ECTree
2.7.7.31 DNA nucleotidylexotransferaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.7.7.31 - DNA nucleotidylexotransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2',3'-dideoxyadenosine 5'-triphosphate + 5'-FAM labelled oligonucleotide
diphosphate + ?
-
-
-
?
2',3'-dideoxycytosine 5'-triphosphate + 5'-FAM labelled oligonucleotide
diphosphate + ?
-
-
-
?
2',3'-dideoxyguanosine 5'-triphosphate + 5'-FAM labelled oligonucleotide
diphosphate + ?
-
-
-
?
2',3'-dideoxythymidine 5'-triphosphate + 5'-FAM labelled oligonucleotide
diphosphate + ?
-
-
-
?
2-pyridone-2'-deoxyriboside triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
-
-
-
?
4-bromo-2-pyridone-2'-deoxyriboside triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
-
-
-
?
4-chloro-2-pyridone-2'-deoxyriboside triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
-
-
-
?
4-hydroxy-2-pyridone-2'-deoxyriboside triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
-
-
-
?
4-thio-2'-deoxyuridine triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
-
-
-
?
5-carboxy-2'-deoxyuridine triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
-
-
-
?
5-cyano-2'-deoxyuridine triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
-
-
-
?
a 2'-deoxyribonucleoside 5'-triphosphate + single stranded DNAn
diphosphate + single stranded DNAn+1
alpha-[2-N-(9-fluorenylmethoxycarbonyl)aminoethylphosphonyl]-beta,gamma-difluoromethylenediphosphonate + DNAn
diphosphate + ?
-
synthetic substrate
-
-
?
alpha-[2-N-(9-fluorenylmethoxycarbonyl)aminoethylphosphonyl]-beta,gamma-diphosphate + DNAn
diphosphate + ?
-
synthetic substrate
-
-
?
B-dUTP + (Cy5-dT10)poly(dTTP)
diphosphate + (Cy5-dT10)poly(dTTP)(B-dUTP)
-
-
-
?
Cy5-dCTP + 2bA FAM oligo
diphosphate + ?
2bA FAM oligo is a primer with FAM at 2 bases from the 3' end (5'-CGCTTGCACAGGTGCGTT/iFluorT/CA-3')
-
-
?
d[5-(3-aminophenyl)]CTP + DNA
diphosphate + ?
5-substituted dCTPs give products elongated by 1 to 5 nucleotides, with most intense bands corresponding to 2-3 extra nucleotides
-
-
?
d[5-(3-aminophenyl)]UTP + DNA
diphosphate + ?
5-substituted dUTPs give products elongated by 4 to 5 nucleotides, the maximum length of the tail is about 14 nucleotides
-
-
?
d[5-(3-nitrophenyl)]CTP + DNA
diphosphate + ?
5-substituted dCTPs give products elongated by 1 to 5 nucleotides, with most intense bands corresponding to 2-3 extra nucleotides
-
-
?
d[5-(3-nitrophenyl)]UTP + DNA
diphosphate + ?
5-substituted dUTPs give products elongated by 4 to 5 nucleotides, the maximum length of the tail is about 14 nucleotides
-
-
?
d[7-deaza-7-(3-aminophenyl)]ATP + DNA
diphosphate + ?
the most intense band observed for reaction with 7-deaza,7-substituted dATPs corresponds to one nucleotide attached, but the products are rather polydisperse in lengths, reaching several tens of extra nucleotides
-
-
?
d[7-deaza-7-(3-nitrophenyl)]ATP + DNA
diphosphate + ?
the most intense band observed for reaction with 7-deaza,7-substituted dATPs corresponds to one nucleotide attached, but the products are rather polydisperse in lengths, reaching several tens of extra nucleotides
-
-
?
d[7-deaza-7-(3-nitrophenyl)]GTP + DNA
diphosphate + ?
most useful substrate, efficiently incorporated by the transferase to form long tail-labels at any oligonucleotide
-
-
?
N4-amino-2'-deoxycytidine triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
-
-
-
?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
a 2'-deoxyribonucleoside 5'-triphosphate + single stranded DNAn
diphosphate + single stranded DNAn+1
-
the enzyme catalyzes the elongation of a DNA with the length of three or more nucleotides by the isothermal and repetitive addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules
-
-
?
a 2'-deoxyribonucleoside 5'-triphosphate + single stranded DNAn
diphosphate + single stranded DNAn+1
-
-
-
?
dATP + DNAn
diphosphate + DNAn+1
terminal transferase activity at the 3'-OH extremity of the nicking site
-
-
?
dATP + DNAn
diphosphate + DNAn+1
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543
terminal transferase activity at the 3'-OH extremity of the nicking site
-
-
?
deoxynucleoside triphosphate + DNA
diphosphate + DNAn+1
-
hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides
-
-
?
deoxynucleoside triphosphate + DNA
diphosphate + DNAn+1
-
enzyme may act as a random-sequence generator in highly localized regions of the DNA, enzyme may be an intracellular marker for a subpopulation of cells
-
-
?
deoxynucleoside triphosphate + DNA
diphosphate + DNAn+1
-
the enzyme has a key role in V(D)J recombination during lymphocyte and repertoire development
-
-
?
deoxynucleoside triphosphate + DNA
diphosphate + DNAn+1
-
involved in creating somatic diversity of immunoglobulin and T-cell receptor genes
-
-
?
deoxynucleoside triphosphate + DNA
diphosphate + DNAn+1
-
roles in DNA replication and repair processes
-
-
?
deoxynucleoside triphosphate + DNA
diphosphate + DNAn+1
-
hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides
-
-
?
deoxynucleoside triphosphate + DNA
diphosphate + DNAn+1
-
hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides
-
-
?
deoxynucleoside triphosphate + DNA
diphosphate + DNAn+1
-
in the presence of Mn2+ yeast telomerase can switch to a template-independent mode of DNA synthesis as terminal transferase
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer: DNase II-treated DNA
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
longer chains interact well with the enzyme, but synthesis rate decreases
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer: oligonucleotides containing at least 3 phosphate groups and a free 3'-hydroxyl end
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
no nucleotide specificity
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer: DNase I-treated DNA
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme also catalyzes diphosphate exchange and pyrophosphorolysis
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
as the initiator chain length is increased to 5-7 nucleotides the polymerization rate also increases
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer: heat-denatured DNA
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
phosphorthioate primers with internucleotide linkages in [RP] configuration are used efficiently, while linkages in [SP] configuration decrease significantly primer extension reaction
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
relative rates of polymerization with several nucleotides
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
longer chains interact well with the enzyme, but synthesis rate decreases
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer: oligonucleotides containing at least 3 phosphate groups and a free 3'-hydroxyl end
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
as the initiator chain length is increased to 5-7 nucleotides the polymerization rate also increases
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme possesses the ability to synthesize in vitro short fragments of DNA in the absence of a primer-template or even a primer or a template in the reaction. The DNA synthesized de novo appears to have an unusual structure
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
low levels of activity with dGTP and oligo(dA), oligo(dC) or oligo(dT)
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the preferred combination is oligo(dT) initiator with dTTP substrate
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer: preferred length 12-18 residues
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
longer chains interact well with the enzyme, but synthesis rate decreases
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer: oligonucleotides containing at least 3 phosphate groups and a free 3'-hydroxyl end
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
as the initiator chain length is increased to 5-7 nucleotides the polymerization rate also increases
-
ir
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the long murine terminal deoxynucleotidyltransferase isoform does not play a role in the control of V(D)J junctional diversity
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
deoxynucleoside triphosphate preference: dGTP, dCTP, dTTP, dATP (42000 MW enzyme)
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
extension of the 3'-hydroxy terminus for approximately 40 nucleotides is achieved if non-denatured DNA and dTTP are used as primer and substrate
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer: polydeoxynucleotides
extension of the 3'-hydroxy terminus for approximately 40 nucleotides is achieved if non-denatured DNA and dTTP are used as primer and substrate
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer: oligodeoxynucleotides
extension of the 3'-hydroxy terminus for approximately 40 nucleotides is achieved if non-denatured DNA and dTTP are used as primer and substrate
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
polymerization is highly efficient if only one of the four deoxyribonucleoside 5'-triphosphates is present
extension of the 3'-hydroxy terminus for approximately 40 nucleotides is achieved if non-denatured DNA and dTTP are used as primer and substrate
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
prefers non-denatured to single-stranded DNA as primer
extension of the 3'-hydroxy terminus for approximately 40 nucleotides is achieved if non-denatured DNA and dTTP are used as primer and substrate
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
extension of the 3'-hydroxy terminus for approximately 40 nucleotides is achieved if non-denatured DNA and dTTP are used as primer and substrate
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
primer required
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reaction is not template directed
-
?
additional information
?
-
-
enzyme additionally has oligodeoxynucleotidyl kinase activity, substrates are triphosphates modified at Palpha,beta,gamma-residues
-
-
?
additional information
?
-
wild type enzyme is unable to incorporate bulky 3'-blocked nucleotides due to steric clashes
-
-
-
additional information
?
-
-
alternative splicing seems to regulate TdT isoform expression. Both long isoforms, hTdTL1 and hTdTL2 have 3'-5' exonuclease activity. Overexpression of the short isoform hTdTS or the long isoform hTdTL2 greatly reduces the efficiency of recombinantion, which is reverted to normal levels by simultaneous expression of both enzymes
-
-
?
additional information
?
-
the wild type enzyme substantially reduces activity on primers with 3' terminal structure (hairpins)
-
-
-
