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2.7.7.4: sulfate adenylyltransferase

This is an abbreviated version!
For detailed information about sulfate adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.4

Reaction

ATP
+
sulfate
=
diphosphate
 +
adenylyl sulfate

Synonyms

3'-phospho-adenosine-5'-phosphosulphate synthase, 3'-phosphoadenosine-5'-phosphosulfate synthase, AcATPS1, adenosine 5'-triphosphate sulphurylase, adenosine 5'-triphosphate-sulfurylase, adenosine triphosphate sulfurylase, adenosine triphosphate sulphurylase, adenosine triphosphate-sulphurylase, adenosine-5'-triphosphate sulfurylase, adenosinetriphosphate sulfurylase, adenylsulfurylase, adenylylsulfate pyrophosphorylase, adenylyltransferase, sulfate, APS1, APS2, At3g22890, ATP sulfurylase, ATP sulfurylase 1, ATP sulfurylase 2, ATP sulfurylase 3, ATP sulfurylase 4, ATP sulfurylase isoform 1, ATP sulfurylase-APS kinase, ATP sulphurylase, ATP-S, ATP-sulfurylase, ATP: sulfate adenylyl transferase, ATPS, ATPS-A, ATPS-B, ATPS1, ATPS2, CysD, dissimilatory ATP sulfurylase, MgATP:sulfate adenylyltransferase, MgATP:sulfate adenylyltransferase,, PAPS synthase, PAPS synthase 1, PAPS synthase 2, PAPS synthase1, PAPS synthase2, PAPSS1, PAPSs2, SAT, sulfate adenylyltransferase, sulfurylase, TtATPS, TTHA0666

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.4 sulfate adenylyltransferase

Purification

Purification on EC 2.7.7.4 - sulfate adenylyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a chloroplastic and a cytosolic enzyme form
-
bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase. Full-length enzyme and its constituent adenosine 5'-phosphosulfate kinase and ATP sulfurylase domains are individually expressed and purified
DEAE-52 cellulose column chromatography, Source 15Q column chromatography, Superdex 200 gel filtration, and hydroxyapatite column chromatography
-
enzyme form ATPSm and ATPSc
-
expressed enzyme is purified by affinity chromatography using glutathione-Sepharose and subsequent ion-exchange chromatography
glutathione Sepharose column chromatography and Superdex 200 gel filtration
glutathione-Sepharose column chromatography and Mono Q column chromatography
hydroxyapatite column chromatography, DEAE cellulose column chromatography, and gel filtration
-
immobilized metal ion affinity chromatography
N-terminal His-tagged protein is passed over a Ni2+-NTA column
partial
protein is purified by sequential chromatographies on hydroxyapatite, DEAE cellulose and gel filtration
-
recombinant enzyme
recombinant His-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration, native enzyme by hydrophobic interaction chromatography, ultrafiltration, dialysis, anion exchange chromatography, ultracentrifugation, and gel filtration
recombinant wild-type and mutant N-terminally truncated and His6-tagged enzymes from Escherichia coli strain Rosetta (DE3) by nicke affinity chromatography and gel filtration