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2.7.7.43: N-acylneuraminate cytidylyltransferase

This is an abbreviated version!
For detailed information about N-acylneuraminate cytidylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.43

Reaction

CTP
+
N-acetylneuraminate
=
diphosphate
 +
CMP-N-acylneuraminate

Synonyms

AaCSS, acylneuraminate cytidyltransferase, CMAS, Cmas1, Cmas2, CMP sialate pyrophosphorylase, CMP-N-acetylneuraminate synthase, CMP-N-acetylneuraminate synthetase, CMP-N-acetylneuraminic acid synthase, CMP-N-acetylneuraminic acid synthetase, CMP-N-acylneuraminic acid synthetase, CMP-NANA synthetase, CMP-Neu5Ac synthetase, CMP-NeuAc synthetase, CMP-NeuNAc synthetase, CMP-SA synthase, CMP-Sia synthetase, CMP-Sia-syn, CMP-sialate synthase, CMP-sialate synthetase, CMP-sialic acid synthetase, CMP-sialic acid synthetase (CSS), CMP-sialic synthetase, CMPsialate pyrophosphorylase, CMPsialate synthase, CNS, CSAS, CSS, cytidine 5'-monophosphate N-acetylneuraminic acid synthetase, cytidine 5'-monophospho-N-acetylneuraminic acid synthetase, cytidine 5'-monophosphosialic acid synthetase, cytidine 5-monophosphate N-acetylneuraminic acid synthetase, cytidine monophosphate N-acetylneuraminic acid synthetase, cytidine monophosphate sialic acid synthetase, cytidine monophosphate-N-acetylneuraminic acid synthetase, cytidine monophosphate-sialic acid synthetase, cytidine monophospho-sialic acid synthetase, cytidine monophosphoacetylneuraminic synthetase, cytidine monophosphosialate pyrophosphorylase, cytidine monophosphosialate synthetase, cytidyltransferase, acylneuraminate, cytidylyltransferase, acetylneuraminate, DmCSAS, DmCSS, hCSS, mCSS, N-Acetyl-neuraminic acid cytidylyltransferase, NmCSS, SaV CSS, sialate cytidylyltransferase, sialic acid cytidylyltransferase, TcCSS

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.43 N-acylneuraminate cytidylyltransferase

Cloned

Cloned on EC 2.7.7.43 - N-acylneuraminate cytidylyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a series of deletions from the 3'-end of the CMP-NeuAc synthetase coding region is constructed and expressed in Escherichia coli
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cloned in Escherichia coli as a His6-tagged fusion protein
cloned in Escherichia coli XL-10
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cloning is achieved by complementation of the Chineses hamster ovary lec32 mutation that causes a deficiency in CMP-N-acetylneuraminate synthetase activity, it also causes polysialic acid to be expressed in the capsule of the CMP-Neu5Ac synthetase negative Escherichia coli mutant EV5
DmCSAS is cloned into the baculovirus vector pBlueBac4.5 (pBlueBac-DmCSAS4) which is used to transfect LEC29.Lec32 cells
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DNA and amino acid sequence determination and analysis, when expressed in Drosophila S2 cells, DmCSS is mainly secreted into the culture medium, although partially detected in Golgi. Transient functional recombinant expression of myc-tagged wild-type and mutant enzymes in LEC29. Lec32 cells which lack a functional CSS, recombinant expression of His-tagged DmCSS in Escherichia coli
DNA and amino acid sequence determination and analysis, when expressed in mammalian and insect cells, recombinant AaCSS shows in vivo and in vitro CSS activities. In contrast, when expressed in bacteria, it lacks CSS activity because the N-terminal hydrophobic region appears to induce protein aggregation, when expressed as C-terminally V5-tagged enzyme in Drosophila S2 cells, AaCSS is predominantly localized in the endoplasmic reticulum, but not in the Golgi. Transient functional recombinant expression of wild-type and mutant myc-tagged enzymes in LEC29. Lec32 cells which lack a functional CSS, recombinant expression of His-tagged AaCSS in Escherichia coli
DNA and amino acid sequence determination and analysis, when expressed in mammalian and insect cells, recombinant TcCSS shows in vivo and in vitro CSS activities. In contrast, when expressed in bacteria, it lacks CSS activity because the N-terminal hydrophobic region appears to induce protein aggregation, when expressed in Drosophila S2 cells, TcCSS is predominantly localized in the endoplasmic reticulum, but not in the Golgi. Transient recombinant expression of myc-tagged enzyme in LEC29.Lec32 cells which lack a functional CSS, recombinant expression of His-tagged TcCSS in Escherichia coli
expressed in CHO cells, HeLa cells, and NIH-3T3 cells
expressed in Drosophila S2 cells or LEC29.Lec32 cells
expressed in Escherichia coli and CHO cells
expressed in Escherichia coli Rosetta 2 DE3 cells
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expressed in Escherichia coli strain BL21
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expressed in Saccharomyces cerevisiae strain Y187
expression in CHO cells
expression in CHO cells or Escherichia coli
expression in Escherichia coli
expression in Escherichia coli, enzyme can function in K1 capsular polysaccharide biosynthesis in Escherichia coli
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gene Cmas, quantitative PCR expression analysis
high-level expression in Escherichia coli
human CMP-N-acetylneuraminic acid synthetase is expressed in tobacco BY2 suspension-cultured cells, enzyme is demonstrated to be active
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the enzyme is subcloned for overexpression in Escherichia coli K12 using the expression vector pKK233-3
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the gene is cloned into a T7 expression vector, the protein is expressed in Escherichia coli
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the N-terminal domain, residues 39-267, and the C-terminal domain, residues 267-432, of CMP-sialic acid synthetase, and CMP-sialic acid synthetase, residues 39-432, are cloned into a pET22b-Strep vector
transfection of NIH-3T3 and EPC cells
transformation of the CMP-NeuNAc defective Escherichia coli K1 strain EV5 with CMP-NeuNAc synthetase from Neisseria meningitidis can complement the defect in Escherichia coli
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