2.7.7.47: streptomycin 3''-adenylyltransferase

This is an abbreviated version!
For detailed information about streptomycin 3''-adenylyltransferase, go to the full flat file.

Reaction

Synonyms

AAD (3''), aadA, AadA6, aminoglycoside (3'')(9) adenylyltransferase, aminoglycoside 3''-adenylyltransferase, aminoglycoside 3'-adenyltransferase, aminoglycoside adenyltransferase, aminoglycoside adenyltransferase A, aminoglycoside adenyltransferase type A, aminoglycoside adenylyltransferase, ANT(3'')-II, ANT(3'')-Ii/AAC(6')-IId, SMATase, str, streptomycin adenylate synthetase, streptomycin adenyltransferase, streptomycin adenylylase, streptomycin adenylyltransferase, streptomycin-spectinomycin adenylyltransferase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.7 Nucleotidyltransferases

                2.7.7.47 streptomycin 3''-adenylyltransferase

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Results	in table
2	Activating Compound
2172	AA Sequence
8	Application
1	CAS Registry Number
17	Cloned(Commentary)
3	Cofactor
4	Crystallization (Commentary)
25	General Information
1	General Stability
13	Inhibitors
6	kcat/KM [mM/s]
6	Ki Value [mM]
11	KM Value [mM]
4	Localization
7	Metals/Ions
4	Molecular Weight [Da]
46	Natural Substrates/ Products (Substrates)
57	Organism
12	PDB ID
5	pH Optimum
4	pI Value
17	Protein Variants
11	Purification (Commentary)
1	Reaction
1	Reaction Type
53	Reference
3	Specific Activity [micromol/min/mg]
79	Substrates and Products (Substrate)
7	Subunits
61	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
3	Temperature Stability [°C]
9	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.7.7.47 - streptomycin 3''-adenylyltransferase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

modeling of enzyme structure

Klebsiella pneumoniae

P08881

723011

homology modeling of structure, and ternary complex with ATP and streptomycin or spectinomycin

Pseudomonas aeruginosa

Q9RGC2

739973

wild type enzyme complexed with ATP, magnesium, and streptomycin, sitting drop vapor diffusion method, using 10% (w/v) PEG 20,000, 20% (v/v) PEG monomethyl ether 550, 0.1 M MOPS/HEPES-Na, pH 7.5. Mutant enzyme E87Q complexed with ATP and streptomycin or dihydrostreptomycin, sitting drop vapor diffusion method, using 20% (w/v) PEG 6000, 0.2 M CaCl2, and 0.1 M HEPES, pH 7.5

Salmonella enterica

-

761481

to 2.5 A resolution. AadA consists of a nucleotidyltransferase domain and an alpha-helical bundle domain. AadA crystallizes as a monomer. ATP binding has to occur before binding of the aminoglycoside substrate, and ATP binding repositions the two domains for aminoglycoside binding in the interdomain cleft

Salmonella enterica subsp. enterica serovar Typhimurium

Q8ZPX9

737356