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2.7.7.48: RNA-directed RNA polymerase

This is an abbreviated version!
For detailed information about RNA-directed RNA polymerase, go to the full flat file.

Word Map on EC 2.7.7.48

Reaction

nucleoside triphosphate
+
RNAn
=
diphosphate
 +
RNAn+1

Synonyms

111 kDa protein, 180 kDa protein, 182 kDa protein, 183 kDa protein, 186 kDa protein, 216.5 kDa protein, 2A protein, 3CD protein, 3D pol, 3D polymerase, 3Dpol, 3Dpol-like protein, 3P complex, 69.6 kDa protein, core protein, core protein VP1, CVB3 RdRp, D-elp1, DENV 3 polymerase, DENV 3 RdRp, DENV RdRp, ego-1, ego-11, FluPol, FMDV 3D, GhRdRP, HC-J4 NS5BDELTA21, HCN NS5B protein, HCV NS5B, HCV NS5B polymerase, HCV RdRp, hepatitis C virus polymerase, HRV16 3Dpol, influenza polymerase PA, inner layer protein VP1, JEV NS5, JEV NS5 protein, JEV RdRp, jRdRp, L protein, L/P RdRP, large structural protein, M1 phosphoprotein, MOP1, More, NgRDR1, NIB, nonstructural phosphoprotein, nonstructural protein, nonstructural protein 12, nonstructural protein 5B, NS5, NS5 polymerase, NS5 protein, NS5 RdRp, NS5B, NS5B enzyme, NS5B polymerase, NS5B protein, NS5B RdRp, NS5B RNA-dependent RNA polymerase, NS5b-directed RNA polymerase, NS7, nsp12, nsp4, nucleocapsid phosphoprotein, nucleotidyltransferase, ribonucleate, RNA-dependent, ORF1, ORF1A, ORF1B, OsRDR6, P protein, P180, P3D, P66, P70, P88 protein, p92, PB1, PB1 proteins, PB2, PB2 proteins, Phage f2 replicase, phi6, phi6 polymerase, picornaviral 3D polymerase, plant-encoded RNA-dependent RNA polymerase 1, Pol, Pol IV, polymerase 3Dpol, polymerase acidic protein, polymerase basic 1 protein, polymerase L, protein 3Dpol, proteins PB1, proteins, PB 2, proteins, specific or class, lambda3, of reovirus, proteins, specific or class, PB 1, proteins, specific or class, PB 2, Q-beta replicase, Qbeta replicase, Qbeta-replicase, RDR, RDR1, RDR1a, RDR1b, RDR1c, RDR1c2, RDR2, RDR6, RDRP, replicase, phage f2, replicase, Qbeta, ribonucleic acid replicase, ribonucleic acid-dependent ribonucleate nucleotidyltransferase, ribonucleic acid-dependent ribonucleic acid polymerase, ribonucleic replicase, ribonucleic synthetase, RNA dependent RNA polymerase, RNA nucleotidyltransferase (RNA-directed), RNA polymerase, RNA polymerase IV, RNA replicase, RNA synthetase, RNA transcriptase, RNA-binding protein, RNA-dependent ribonucleate nucleotidyltransferase, RNA-dependent RNA polymerase, RNA-dependent RNA polymerase 1, RNA-Dependent RNA Polymerase 2, RNA-dependent RNA polymerase 6, RNA-dependent RNA polymerase NIb, RNA-dependent RNA polymerase NS5B, RNA-dependent RNA polymerases, RNA-dependent RNA replicase, RNA-dependent RNA-polymerase, RNA-directed RNA polymerase, RNA-directed RNA polymerase L, RNAdependent RNA polymerase, rrf-1, RRF-3, RrpC, RSV RdRp, SARS-CoV-2 RdRp, SARS-CoV-2 RNA polymerase, SARSCoV-2 polymerase, SARSCoV-2-core polymerase complex, self-priming RdRp, sigma NS protein, transcriptase, VP1, VP1 protein, WNV NS5

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.48 RNA-directed RNA polymerase

Substrates Products

Substrates Products on EC 2.7.7.48 - RNA-directed RNA polymerase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(dT)12 + UTP
diphosphate + ?
show the reaction diagram
-
assay uses poly(A) as an RNA template and oligo(dT)12-18 as the primer. Enzyme is strictly dependent on the presence of primer
-
-
?
2'-C-methyl-ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
-
-
ir
5-fluorouridine triphosphate + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
the enzyme incorporates 5-fluorouridine monophosphate during RNA elongation in place of UMP or CMP using homopolymeric and heteropolymeric templates. Incorporation of 5-fluorouridine monophosphate does not prevent chain elongation, and, in some sequence contexts, it favors misincorporations at downstream positions. 5-Fluorouridine monophosphate is incorporated into the nascent RNA and occupies the new 3'-end of the primer at the active site of the enzyme. 5-Fluorouridine monophosphate establishes a Watson and Crick pair with the corresponding acceptor AMP in the template strand and an additional hydrogen bond with Ser304 of the polymerase. Further interactions, similar to those observed with standard nucleotides, contribute also to stabilize 5-fluorouridine monophosphate in the 3'-terminus of the RNA. When present in the template, 5-fluorouridine monophosphate directs the incorporation of AMP and GMP, with ATP being a more effective substrate than GTP. The misincorporation of GMP is 17fold faster opposite 5-fluorouridine than opposite U in the template. But Incorporated 5-fluorouridine monophosphate is not a chain terminator during RNA elongation
RNA with incoporated 5-fluorouridine phosphate
-
?
ara-ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
-
-
ir
ATP + poly(U)
diphosphate + ?
show the reaction diagram
ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
ATP + sshRNAn
diphosphate + sshRNAn+1
show the reaction diagram
-
-
-
-
?
CTP + RNA9
diphosphate + RNA10
show the reaction diagram
AJ242654
-
-
-
?
CTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
CTP + sshRNAn
diphosphate + sshRNAn+1
show the reaction diagram
-
-
-
-
?
dATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
-
-
ir
GTP + poly(C)
diphosphate + ?
show the reaction diagram
GTP + poly(C)
diphosphate + poly(C)n+1
show the reaction diagram
-
-
-
-
?
GTP + poly(C)n
diphosphate + poly(C)n+1
show the reaction diagram
-
-
-
-
?
GTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
GTP + sshRNAn
diphosphate + sshRNAn+1
show the reaction diagram
-
-
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
show the reaction diagram
remdesivir triphosphate + RNAn
diphosphate + RNAn 3'-remdesivir
show the reaction diagram
remdesivir triphosphate + RNAn
diphosphate + RNAn+1
show the reaction diagram
remdesivir triphosphate is effective in combating COVID-19 because it is a better substrate than ATP for the viral RNA-dependent RNA polymerase
-
-
?
rGTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
ribavirin triphosphate + RNAn
?
show the reaction diagram
ribavirin is a guanosine analogue that can be a substrate for the viral RNA polymerase. HCV is genetically variable, and this genetic variation can affect the polymerase's use of ribavirin triphosphate, overview
-
-
?
UTP + poly(A)n
diphosphate + poly(A)n+1
show the reaction diagram
-
-
-
-
?
UTP + RNAn
diphosphate + RNAn+1
show the reaction diagram
UTP + sshRNAn
diphosphate + sshRNAn+1
show the reaction diagram
-
-
-
-
?
additional information
?
-