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2.7.7.49: RNA-directed DNA polymerase

This is an abbreviated version!
For detailed information about RNA-directed DNA polymerase, go to the full flat file.

Word Map on EC 2.7.7.49

Reaction

a 2'-deoxyribonucleoside 5'-triphosphate
+
DNAn
=
diphosphate
 +
DNAn+1

Synonyms

Bst DNA polymerase, CS5 pol, DNA nucleotidyltransferase (RNA-directed), DNA polymerase, DNA polymerase I, FeLV RT, FIV RT, FV Pol, FV reverse transcriptase, Gag-Pol, HBV-pol, HIV reverse transcriptase, HIV-1 M RT, HIV-1 O RT, HIV-1 R, HIV-1 reverse transcriptase, HIV-1 RT, HIV-reverse transcriptase, HIV-RT, human hepatitis B virus polymerase, human immunodeficiency virus type 1 reverse transcriptase, iScript enzyme, K4 polymerase, K4PolI, M-MuLV reverse transcriptase, MMLV RT, Moloney Murine leukemia virus reverse transcriptase, Moloney murine leukemia virus RT, MoMLV RT, More, MuLV RT, MX162-RT, MX65-RT, nucleoside reverse transcriptase, nucleotidyltransferase, deoxyribonucleate, RNA-dependent, p66 RT, P72, PFV RT, Pol, polymerase/reverse transcriptase, prototype foamy virus reverse transcriptase, R2-RT, reverse transcriptase, reverse transcriptase/RNA dependent DNA polymerase, reverse-transcriptase, revertase, RNA dependent DNA polymerase, RNA revertase, RNA-dependent DNA polymerase, RNA-instructed DNA polymerase, RT, SFV RT, simian foamy virus reverse transcriptase, SS RT, SuperScript I reverse transcriptase, Superscript II, SUPERSCRIPT II reverse transcriptase, T. Z05 pol, telomerase, telomerase catalytic subunit, telomerase reverse transcriptase, TERT, Tgo-Pol, xenotropic murine leukemia virus-related virus reverse transcriptase, XMRV RT, XNA reverse transcriptase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.49 RNA-directed DNA polymerase

Crystallization

Crystallization on EC 2.7.7.49 - RNA-directed DNA polymerase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structures of natural Bst DNA polymerase that capture the post-translocated product of DNA synthesis on templates composed entirely of 2'-deoxy-2'-fluoro-beta-D-arabino nucleic acid and alpha-L-threo-furanosyl nucleic acid
in complex with inhibitor TMC278, hanging drop vapour diffusion method, using 9% to 12% PEG 8000, 50 mM imidazole pH 6.0-6.8, 10 mM spermine, 15 mM MgSO4 and 100 mM ammonium sulfate
molecular docking of inhibitor emetine
-
molecular modeling of complexes with inhibitors, e.g. 6-([2-[(4-cyanophenyl)amino]pyrimidin-4-yl]amino)-5,7-dimethylnaphthalene-2-carbonitrile, 6-([4-[(4-cyanophenyl)amino]-1,3,5-triazin-2-yl]amino)-5,7-dimethylnaphthalene-2-carbonitrile
-
the development of a modified DNA aptamer that binds HIV-1 reverse transcriptase (RT) with ultra-high affinity enables the X-ray structure determination of an HIV-1 RT-DNA complex to 2.3 A resolution without the need for an antibody Fab fragment or RT-DNA crosslinking. The 38-mer hairpin-DNA aptamer has a 15 base-pair duplex, a three-deoxythymidine hairpin loop, and a five-nucleotide 5'-overhang. The aptamer binds RT in a template-primer configuration with the 3'-end positioned at the polymerase active site and has 2'-O-methyl modificationsat the second and fourth duplex template nucleotides that interact with the p66 fingers and palm subdomains. This structure represents the highest resolution RT-nucleic acid structure to date. The RT-aptamer complex is catalytically active and can serve as a platform for studying fundamental RT mechanisms and for development of anti-HIV inhibitors through fragment screening and other approaches
the engineered reverse transcriptase protein (Q258C and C280S mutations) cross-linked to the dsDNA primer/template (20-mer/27-mer) containing the N2-cystamine 2'-deoxyguanosine six bases upstream from the priming site (P site) was used for the crystallographic studies
crystal structure of inhibitor 6-([4-[(4-cyanophenyl)amino]-1,3,5-triazin-2-yl]amino)-5,7-dimethylnaphthalene-2-carbonitrile bound to the active site of an isolated RNase H domain of HIV-1 reverse transcriptase at a resolution limit of 1.71 A
fragment screening by X-ray crystallography revealed seven new inbhibitor binding sites within HIV-1 reverse transcriptase, of which three sites, i.e. the Knuckles, the NNRTI Adjacent, and the Incoming Nucleotide Binding sites are inhibitory in an enzymatic assay
crystal structure of the full-length Moloney murine leukemia virus reverse transcriptase at 3.0 A resolution, hanging-drop vapour-diffusion method
-