2.7.7.49: RNA-directed DNA polymerase
This is an abbreviated version!
For detailed information about RNA-directed DNA polymerase, go to the full flat file.
Word Map on EC 2.7.7.49
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2.7.7.49
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telomeres
-
htert
-
antiretroviral
-
immortalization
-
nnrti
-
ribonucleoprotein
-
viruses
-
virological
-
hiv-infected
-
lymphocyte
-
nonnucleoside
-
zidovudine
-
marrow
-
lengthen
-
strand
-
subtype
-
carcinogenesis
-
tumour
-
efavirenz
-
retroviral
-
rnase
-
nevirapine
-
tumorigenesis
-
lamivudine
-
g-quadruplexes
-
anti-hiv
-
drug-resistant
-
cd4
-
c-myc
-
single-stranded
-
integrase
-
first-line
-
alt
-
tenofovir
-
karyotype
-
polymerases
-
lifespan
-
retroviruses
-
ritonavir
-
proviral
-
polyproteins
-
virion
-
treatment-naive
-
dntp
-
indinavir
-
erosion
-
duplex
-
p16ink4a
-
hiv-positive
-
papillomavirus
-
medicine
-
synthesis
-
drug development
-
analysis
- 2.7.7.49
-
telomeres
-
htert
-
antiretroviral
-
immortalization
-
nnrti
- ribonucleoprotein
- viruses
-
virological
-
hiv-infected
- lymphocyte
-
nonnucleoside
- zidovudine
- marrow
-
lengthen
- strand
- subtype
- carcinogenesis
- tumour
- efavirenz
-
retroviral
- rnase
- nevirapine
- tumorigenesis
- lamivudine
-
g-quadruplexes
-
anti-hiv
-
drug-resistant
- cd4
- c-myc
-
single-stranded
-
integrase
-
first-line
-
alt
- tenofovir
-
karyotype
- polymerases
-
lifespan
- retroviruses
- ritonavir
-
proviral
- polyproteins
- virion
-
treatment-naive
- dntp
- indinavir
-
erosion
- duplex
- p16ink4a
-
hiv-positive
- papillomavirus
- medicine
- synthesis
- drug development
- analysis
Reaction
Synonyms
Bst DNA polymerase, CS5 pol, DNA nucleotidyltransferase (RNA-directed), DNA polymerase, DNA polymerase I, FeLV RT, FIV RT, FV Pol, FV reverse transcriptase, Gag-Pol, HBV-pol, HIV reverse transcriptase, HIV-1 M RT, HIV-1 O RT, HIV-1 R, HIV-1 reverse transcriptase, HIV-1 RT, HIV-reverse transcriptase, HIV-RT, human hepatitis B virus polymerase, human immunodeficiency virus type 1 reverse transcriptase, iScript enzyme, K4 polymerase, K4PolI, M-MuLV reverse transcriptase, MMLV RT, Moloney Murine leukemia virus reverse transcriptase, Moloney murine leukemia virus RT, MoMLV RT, More, MuLV RT, MX162-RT, MX65-RT, nucleoside reverse transcriptase, nucleotidyltransferase, deoxyribonucleate, RNA-dependent, p66 RT, P72, PFV RT, Pol, polymerase/reverse transcriptase, prototype foamy virus reverse transcriptase, R2-RT, reverse transcriptase, reverse transcriptase/RNA dependent DNA polymerase, reverse-transcriptase, revertase, RNA dependent DNA polymerase, RNA revertase, RNA-dependent DNA polymerase, RNA-instructed DNA polymerase, RT, SFV RT, simian foamy virus reverse transcriptase, SS RT, SuperScript I reverse transcriptase, Superscript II, SUPERSCRIPT II reverse transcriptase, T. Z05 pol, telomerase, telomerase catalytic subunit, telomerase reverse transcriptase, TERT, Tgo-Pol, xenotropic murine leukemia virus-related virus reverse transcriptase, XMRV RT, XNA reverse transcriptase
ECTree
2.7.7.49 RNA-directed DNA polymeraseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.7.7.49 - RNA-directed DNA polymerase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
((1s,3s)-3-(7-amino-2H-2,3,5,6-tetraazabenzo[cd]azulen-2-yl)cyclobutyl)methyl tetrahydrogen triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
((2R,3S,5R)-3-hydroxy-5-(6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)tetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate
diphosphate + DNAn+1
-
-
-
-
?
((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-yloxy)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
((2R,3S,5R)-5-(6-amino-8-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
(-)-beta-2',3'-dideoxy-3'-thiacytidine triphosphate + 2-naphthyl-Tyr115 of p66 reverse transcriptase
?
-
-
-
-
?
(-)-beta-2',3'-dideoxy-3'-thiacytidine triphosphate + aminomethyl-Phe115 of p66 reverse transcriptase
?
-
-
-
-
?
(-)-beta-2',3'-dideoxy-3'-thiacytidine triphosphate + DNAn
diphosphate + ?
-
-
-
-
?
(-)-beta-2',3'-dideoxy-3'-thiacytidine triphosphate + Tyr115 of p66 reverse transcriptase
?
-
-
-
-
?
2'-deoxyribonucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme mutant N483Q/S486Q/T539N/Y545Q/D547T/P548Q/A570Q/D578Q/A597T/W604R/S612N/V730L/R736Q/S739N/M747R
-
-
?
2'-fluoro-N-cyclobutyladenosine triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate + DNAn
diphosphate + ?
-
incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue
-
-
?
3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate + DNAn
diphosphate + ?
-
incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue
-
-
?
3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate + DNAn
diphosphate + ?
-
incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue
-
-
?
3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate + DNAn
diphosphate + ?
-
incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue
-
-
?
3'-azido-2',3'-dideoxyadenosine triphosphate + DNAn
diphosphate + DNAn+1
-
incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue
-
-
?
N-cyclobutyladenosine triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
N-cyclobutyladenosine-phosphonyl diphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
thymidine-5'-O-1-thiotriphosphate + DNAn
diphosphate + DNAn+1
-
enzyme exhibits a strong preference to incorporate Sp-TTP alphaS isomer over Rp-TTP alphaS isomer in the presence of Mg2+. This stereoselective preference is decreased when Mg2+ is replaced with Mn2+ and Co2+. The enzyme exhibited no phosphorothioate elemental effects for the incorporation of Sp-TTP alphaS, but large elemental effects were calculated for Rp-TTP alphaS for each of the metals tested
-
-
?
2'-deoxyadenosine 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
2'-deoxyadenosine 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
2'-deoxycytidine 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
2'-deoxycytidine 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
2'-deoxyguanosine 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
2'-deoxyguanosine 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
2'-deoxythymidine 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
2'-deoxythymidine 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
diphosphate + DNAn+1
the enzyme exhibits an innate ability to reverse transcribe RNA and other synthetic congeners (XNAs) into DNA. Analysis of the active site of the enzyme reveals the importance of structural plasticity as a possible mechanism for XNA-dependent DNA synthesis
-
-
?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
exploration of the reaction mechanism of HIV reverse transcriptase with a nucleotide substrate
-
-
?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
diphosphate + DNAn+1
-
globally fitting transient kinetic data are collected over a range of substrate concentrations and temperatures to derive kinetic and thermodynamic parameters governing enzyme specificity. Nucleotide binding is exothermic but is marginally stable relative to the physiological substrate concentrations, but substrate binding triggers an enzyme conformational change, which is the major determinant of specificity
-
-
?
dATP + DNAn
diphosphate + DNAn+1
-
RNA-dependent DNA synthesis
-
-
?
dATP + DNAn
diphosphate + DNAn+1
-
enzyme mutant N483Q/S486Q/T539N/Y545Q/D547T/P548Q/A570Q/D578Q/A597T/W604R/S612N/V730L/R736Q/S739N/M747R
-
-
?
dCTP + DNAn
diphosphate + DNAn+1
-
enzyme mutant N483Q/S486Q/T539N/Y545Q/D547T/P548Q/A570Q/D578Q/A597T/W604R/S612N/V730L/R736Q/S739N/M747R
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
efficiency of natural and synthetic templates
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme accepts a variety of natural RNA templates, but shows a preference for oncogenic virus RNA. RNA from other oncogenic viruses is as efficient as AMV RNA. Homopolymeric duplexes are exceptionally good templates, stimulating synthesis 100fold greater than natural RNA or DNA. The enzyme requires a primer
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
affinity of the enzyme for (U)n and a series of (U)n analogs
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the polymerase requires a primer strand with free 3'-hydroxyl group and a template strand to direct DNA synthesis
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme requires a 3'-OH group on a primer and carrying out synthesis from the 5' to the 3' end of the molecule, that is by addition of nucleoside monophosphates at the 3'-OH end of the primer. Poly(rA) is almost totally inactive as a template until a primer, either poly(dT) or oligo(dT) is added
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the alpha enzyme form is more active in the single-strand cDNA-directed synthesis of double-stranded cDNA-directed synthesis of double-stranded DNA than the other 2 enzyme forms
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme accepts a variety of natural RNA templates, but shows a preference for oncogenic virus RNA. RNA from other oncogenic viruses is as efficient as AMV RNA. Homopolymeric duplexes are exceptionally good templates, stimulating synthesis 100fold greater than natural RNA or DNA. The enzyme requires a primer
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme appears to be required very early after infection to synthesize proviral DNA
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
R2-RT is capable of efficiently utilizing single-stranded DNA (ssDNA) as a template. The processivity of the enzyme on ssDNA templates is higher than its processivity on RNA templates. This finding suggests that R2-RT is also capable of synthesizing the second DNA strand during retrotransposition
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
Chicken syncytial virus
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
Chicken syncytial virus
-
the enzyme appears to be required very early after infection to synthesize proviral DNA
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme appears to be required very early after infection to synthesize proviral DNA
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the purified enzyme can synthesize DNA using RNA as a template and a synthetic oligodeoxynucleotide as a primer: cDNA can be synthesized using the Escherichi coli 5S RNA as template and a 15-base synthetic oligonucleotide complementary to the 3'-end of the 5S RNA as a primer. The enzyme can also produce a full-length cDNA using a 50-base synthetic DNA as a template and a synthetic oligonucleotide complementary to the 3'-end of the template as a primer
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
RNA-dependent and DNA-dependent DNA polymerase activity. The p66/p51 heterodimer can perform strand displacement DNA synthesis of approximately 300 bases. The homodimer p66 alone can carry out limited strand displacement DNA synthesis, but this activity is stimulated by the p51 subunit at a molar ratio of one molecule of p55 to five molecules of p51. The homodimer p51 itself is unable to fill a small gap of 26 nucleotides in a double-stranded DNA substrate and is not active by itself in strand displacement DNA synthesis
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme might play a role in normal differentiation
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
Hamster leukemia virus
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
Hamster leukemia virus
-
the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
Hamster leukemia virus
-
enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
Hamster leukemia virus HaLV
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
poly(dA)/oligo(dT)12-18 and poly(rA)/oligo(dT)12-18 as template-primers
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
691140, 691152, 691632, 691882, 692539, 692555, 692567, 692580, 692582, 692597, 693312, 693914, 694207, 694309, 694504, 695141, 695224
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
DNA synthesis of the recombinant enzyme is higher on poly(rA)*oligo(dT)12 than on poly(rC)*oligo(dG). The activity on poly[d(A-T)] is noticeably lower than that on poly(rA)*oligo(dT)12
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
DNA polymerase Pol gamma also catalyzes reverse transcription with a slightly higher efficiency than HIV-1 reverse transcriptase
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
DNA polymerase Pol gamma also catalyzes reverse transcription with a slightly higher efficiency than HIV-1 reverse transcriptase. RNA-primed DNA synthesis activity is required for initiation of mtDNA replication. Poly gamma holoenzyme is capable of performing this reaction at a physiologically releavant rate
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
HTLV-III
-
the enzyme transcribs (rA)n*(dT)12, (rAm)n*(dT)12, (rC)n*(dG)12 and (rCm)n*(dG)12. The enzyme catalyzes transcription of the 70S RNA from SSAV. (RC)n*(dG)12-dependent activity is several fold higher than that catalyzed by (rA)n*(dT)12 and is strictly Mg2+-dependent
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
661221, 662208, 662652, 690316, 690317, 691234, 691261, 691778, 691942, 692252, 692800, 693371, 693584, 693910, 695260, 695261, 714972, 721311, 721815, 722241, 722692, 723089
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
with RNA-directed DNA synthesis, the rate-limiting step occurs after the phosphodiester bond formation while with DNA template it occurs at the dNTP binding step
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
bis-(2'-deoxynucleoside)5,5'-tetraphosphates and bis-(2'-deoxynucleoside)5',5'-triphosphates are effective substrates for DNA elongation
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
mechenism
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
poly(rA)n¥oligo(dT)12-18
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
reverse transcriptase has RNA-dependent and DNA-dependent DNA polymerase activity
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
RNA-dependent DNA synthesis
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
Ext-T DNA 23-mer primer annealed to the RNA 40-mer template
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
Human T-cell lymphotropic virus/lymphadenopathy-associated virus
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
Lymphadenopathy associated virus
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme appears to be required very early after infection to synthesize proviral DNA
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
wild-type Moloney murine leukemia virus reverse transcriptase selectively uses deoxyribonucleotides over ribonucleotides as substrates
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
Td26/50-Cy3-Pd18b
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme appears to be required very early after infection to synthesize proviral DNA
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
due to its low dNTP binding affinity, the dNTP binding step becomes rate-limiting in the multiple rounds of the dNTP incorporation by MuLV RT. The active site of MuLV RT has an intrinsically low dNTP binding affinity, compared with HIV-1 RT. In addition, instead of the misinsertion step, the mismatch extension step, which varies between MuLV and HIV-1 RTs, contributes to their fidelity differences
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme synthesizes full-length cDNA copies of in vitro transcripts beginning at the 3'-end and has a preference for transcripts having the 3'tRNA-like structure. The enzyme begins cDNA synthesis directly opposite the 3'-terminal nucleotide of the template RNA. The activity with poly(rC) alone is about 5% of that with poly(rC)*oligo(dG), efficient use of the substrate is dependent on the primer
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
prototype foamy virus
-
heteropolymeric single stranded M13 substrate
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme requires a 3'-OH group on a primer and carrying out synthesis from the 5' to the 3' end of the molecule, that is by addition of nucleoside monophosphates at the 3'-OH end of the primer. Poly(rA) is almost totally inactive as a template until a primer, either poly(dT) or oligo(dT) is added
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme appears to be required very early after infection to synthesize proviral DNA
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme appears to be required very early after infection to synthesize proviral DNA
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme requires a 3'-OH group on a primer and carrying out synthesis from the 5' to the 3' end of the molecule, that is by addition of nucleoside monophosphates at the 3'-OH end of the primer. Poly(rA) is almost totally inactive as a template until a primer, either poly(dT) or oligo(dT) is added
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
all four deoxyribonucleotide triphosphates are required for full activity, some activity is present when only three deoxyribonucleotide triphosphates are added and 10-20% of full activity is still present with only two deoxyribonucleotide triphosphates
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme plays a central role in transposition of retroelements
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
telomerase is the cellular RNA-dependent DNA polymerase that uses an integral RNA template to synthesize telomeric DNA repeats at the ends of linear chromosomes. Dimerization as a functionally conserved feature of the RNA templates utilized by reverse transcriptases
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
heteropolymeric single stranded M13 substrate
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme shows both RNA-dependent and DNA-dependent DNA synthesis activity and an associated RNAse H activity
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme prefers the template-primer poly(rA)*oligo(dT) over poly(rC)*oligo(dG). With poly(rCm)*oligo(dG) only marginal activity is detected, and no activity is measured with poly(dA)*oligo(dT)
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme appears to be required very early after infection to synthesize proviral DNA
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
DNA polymerase (PolA) and topoisomerase I (TopA) proteins exhibit highly efficient reverse transcriptase activity in addition to their predicted functions
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
DNA polymerase (PolA) and topoisomerase I (TopA) proteins exhibit highly efficient reverse transcriptase activity in addition to their predicted functions
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
DNA polymerase shows significant reverse-transcriptase activity in presence of Mg2+
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
RNA-dependent DNA polymerase activity by enzyme mutants T326A, L329A, Q384A, F388A, M408A, Y438A, L329A/Q384A, L329A/Y438A, and Q384A/Y438A, not by the wild-type enzyme
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
RNA-dependent DNA polymerase activity by enzyme mutants T326A, L329A, Q384A, F388A, M408A, Y438A, L329A/Q384A, L329A/Y438A, and Q384A/Y438A, not by the wild-type enzyme
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
Td26/50-Cy3-Pd18b
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
DNA polymerase shows significant reverse-transcriptase activity in presence of Mg2+
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
poly(rC)*p(dG)(12-18)
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
DNA polymerase shows significant reverse-transcriptase activity in presence of Mg2+
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
poly(rC)*p(dG)(12-18)
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
-
DNA polymerase shows significant reverse-transcriptase activity in presence of Mg2+
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
[Caldibacillus] cellulovorans CompA.2
-
DNA polymerase shows significant reverse-transcriptase activity in presence of Mg2+
-
-
?
dGTP + DNAn
diphosphate + DNAn+1
-
RNA-dependent DNA synthesis
-
-
?
dTTP + DNAn
diphosphate + DNAn+1
-
-
-
?
dTTP + DNAn
diphosphate + DNAn+1
-
incorporation of dTTP into poly(rA)-p(dT)15
-
-
?
dTTP + DNAn
diphosphate + DNAn+1
-
incorporation of dTTP into poly(rA)-p(dT)15
-
-
?
dTTP + DNAn
diphosphate + DNAn+1
-
incorporation of dTTP into poly(rA)-p(dT)45
-
-
?
dTTP + DNAn
diphosphate + DNAn+1
prototype foamy virus
-
homopolymeric substrate poly(rA)/oligo(dT)
-
-
?
dTTP + DNAn
diphosphate + DNAn+1
-
homopolymeric substrate poly(rA)/oligo(dT)
-
-
?
dTTP + DNAn
diphosphate + DNAn+1
-
enzyme mutant N483Q/S486Q/T539N/Y545Q/D547T/P548Q/A570Q/D578Q/A597T/W604R/S612N/V730L/R736Q/S739N/M747R
-
-
?
additional information
?
-
-
reverse transcriptases perform template switches when there is a very short (two-nucleotide) complementarity between the 3' ends of the primer (donor) strand and the DNA or RNA template acceptor strands. Combined two-step clamp/DNA polymerase activity, where the initial clamp is followed by DNA synthesis, overview
-
-
?
additional information
?
-
-
the reverse transcriptase activity of DNA polymerase gamma is not likely to contribute significantly to the biology of mitochondrial DNA replication
-
-
?
additional information
?
-
-
the reverse transcriptase activity of DNA polymerase gamma: in comparison with the kinetic parameters observed with a DNA template, the rate of correct deoxynucleotide incorporation is reduced 25fold, whereas the dissociation constant for nucleotide binding is increased 4fold
-
-
?
additional information
?
-
-
telomerase function comprises lengthening of telomeres, enhancement of DNA repair, promotion of cell growth, modulation of mitochondrial functions under oxidative stress, inhibition of apoptosis, promotion of stem cell proliferation, suppression of DNA damage checkpoints
-
-
?
additional information
?
-
-
reverse transcriptase supports RNA-directed DNA synthesis, DNA-directed DNA synthesis and DNA-directed RNA hydrolysis, the enzyme adopts opposite binding orientations on duplexes containing DNA or RNA primers, directing DNA synthesis or RNA hydrolysis activity respectively, binding orientation determines enzymatic activity of reverse transcriptase
-
-
?
additional information
?
-
-
bis-(2'-deoxynucleoside)5,5'-tetraphosphates and bis-(2'-deoxynucleoside)5',5'-triphosphates are effective substrates for DNA elongation
-
-
?
additional information
?
-
-
can use both RNA and DNA as a template for DNA synthesis and can cleave RNA within an RNA/DNA hybrid (RNase H activity)
-
-
?
additional information
?
-
-
uses the cellular tRNALys,3 molecule as primer
-
-
?
additional information
?
-
-
ribonucleoside triphosphate are efficiently incorporated into DNA in the macrophage but not in the T cell environment, detailed overview. HIV-1 RT initiates both (-)- and (+)-proviral DNA synthesis using RNA primers (e.g. tRNA3Lys and polypurine tract RNA primer) containing 3'-end ribonucleoside monophosphates during viral replication
-
-
?
additional information
?
-
-
the enzyme performs DNA-dependent DNA synthesis and RNA-dependent DNA synthesis, see also EC 2.7.7.7
-
-
?
additional information
?
-
-
the viral DNA polymerase activity can be both RNA and DNA dependent, see also EC 2.7.7.7
-
-
?
additional information
?
-
-
HIV-1 RT has high substrate affinity and low susceptibility to formamide, and low fidelity. Extremely high fidelity is not required for detection of a target RNA in RT-polymerase chain reaction and RNA-specific amplification, and an isothermal reaction that is widely used in clinical diagnosis, in which RT synthesizes promoter-bearing double-stranded DNA with the help of its RNase H activity
-
-
?
additional information
?
-
-
N-cyclobutyladenosine analogues can act as substrates for incorporation by HIV-1 RT and be a potential scaffold for HIV inhibitors, overview
-
-
?
additional information
?
-
AF324493
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-) ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-) ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-) ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
-
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-) ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
AF324493
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-) ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview.. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-) ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview.. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-) ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview.. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
-
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-) ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview.. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
AF324493
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-)ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-)ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-)ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
-
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-)ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
-
reverse transcriptases perform template switches when there is a very short (two-nucleotide) complementarity between the 3' ends of the primer (donor) strand and the DNA or RNA template acceptor strands. Combined two-step clamp/DNA polymerase activity, where the initial clamp is followed by DNA synthesis, overview
-
-
?
additional information
?
-
-
binding affinity Kd of HIV-1 reverse transcriptase for non-canonical rNTPs is 1.4- to 43fold lower, and the rNTP rate of incorporation is 15- to 1551fold slower than for dNTP, suggesting that reverse transcriptase is more selective for incorporation of dNTPs rather than rNTPs. HIV-1 reverse transcriptase selectivity for dNTP versus rNTP is the lowest for ATP
-
-
?
additional information
?
-
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-) ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
recombinant subtype B and C HIV-1 reverse transcriptases show similar enzyme activities and efficiency of tRNA-primed (-) ssDNA synthesis, processivity, fidelity and RNase H activity, as well as susceptibilities to drugs, overview.. The enzyme also exhibits RNase H activity, EC 3.1.13.2
-
-
?
additional information
?
-
-
HIV-1 RT has high substrate affinity and low susceptibility to formamide
-
-
?
additional information
?
-
-
RNA-DNA hybrid substrate preparation by annealing the RNA oligonucleotide LA-237 with a 1.2fold excess of the complementary DNA oligonucleotide JV-08
-
-
?
additional information
?
-
-
reverse transcriptases perform template switches when there is a very short (two-nucleotide) complementarity between the 3' ends of the primer (donor) strand and the DNA or RNA template acceptor strands. Combined two-step clamp/DNA polymerase activity, where the initial clamp is followed by DNA synthesis, overview
-
-
?
additional information
?
-
-
elongates telomeres to tolerate mutations in the telomeric template
-
-
?
additional information
?
-
-
the N-terminal protease domain of the reverse transcriptase also shows polymerase activity
-
-
?
additional information
?
-
-
no saturation is observed for extension on DNA templates
-
-
?
additional information
?
-
-
no saturation is observed for extension on DNA templates
-
-
?
additional information
?
-
-
DNA-dependent DNA polymerase commonly accepts DNA and dNTP and excludes RNA and rNTP, but some enzyme mutants also show RNA-dependent DNA polymerase activity as reverse transcriptases, overview. Reverse transcriptase is the enzyme that catalyzes DNA polymerization using RNA as a template, i.e. RNA-dependent DNA polymerase, see for EC 2.7.7.49
-
-
?
additional information
?
-
-
DNA-dependent DNA polymerase commonly accepts DNA and dNTP and excludes RNA and rNTP, but some enzyme mutants also show RNA-dependent DNA polymerase activity as reverse transcriptases, overview. Reverse transcriptase is the enzyme that catalyzes DNA polymerization using RNA as a template, i.e. RNA-dependent DNA polymerase, see for EC 2.7.7.49
-
-
?
additional information
?
-
-
the mutant enzyme shows single nucleotide additions with dCTP, dATP and dTTP, but not with dGTP as it results in addition of two successive base incorporations on the chosen template 2 hybridised to the DNA primer 1, thereby invalidating the single-turnover kinetic model, Michaelis-Menten mechanism, overview
-
-
?
