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2.7.7.50: mRNA guanylyltransferase

This is an abbreviated version!
For detailed information about mRNA guanylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.50

Reaction

GTP
+
(5')ppPur-mRNA
=
diphosphate
+
G(5')pppPur-mRNA

Synonyms

A103R, A103R protein, cap guanylyltransferase-methyltransferase, capping enzyme, capping enzyme guanylyltransferase, Ceg1, CET1, CmCeg1, D1 protein, GDP polyribonucleotidyltransferase, GlCeg1, GTase, GTP-RNA guanylyltransferase, GTP:RNA GTase, guanylyltransferase, guanylyltransferase mRNA capping, HCE, L protein, Mce1, messenger RNA guanylyltransferase, More, mRNA capping enzyme, mRNA guanylyl transferase, mRNA-cap, mRNA-capping enzyme, NAD-decapping enzyme, nsP1, NudC, PRNTase, protein lambda2, RNA capping enzyme, RNA guanylyltransferase, RNGTT, TbCgm1, TTM-type RTPase-GTase, VACWR106, VP3

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.50 mRNA guanylyltransferase

Cloned

Cloned on EC 2.7.7.50 - mRNA guanylyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, expression of truncation mutant in Escherichia coli BL21(DE3) and wild-type and mutant K294A enzyme in Saccharomyces haploid deficient mutant strain, functional complementation of the latter by wild-type enzyme
expession in Escherichia coli
-
expressed in Escherichia coli BL21(ROS2) cells
-
expressed in Escherichia coli DE3 cells
-
expressed in Escherichia coli One Shot BL21 Star (DE3) cells
-
expressed in Sf21 insect cells
Lyssavirus rabies
-
expression in Escherichia coli
expression in HeLa cells via vaccinia virus strain WR into whose thymidine kinase gene the reovirus lambda2 genome segment has been inserted, possesses neither nucleoside nor RNA 5'-triphosphatase activity nor methyltransferase activity
-
expression in Saccharomyces cerevisiae
-
expression in Spodoptera frugiperda cells via infection with baculovirus
bluetongue virus serotype 10
-
expression of His-tagged full length enzyme, residues 211-597, comprising the catalytical domain, and residues 1-210 in Escherichia coli BL21(DE3)
expression of viral D1 and D12 orfs encoded subunits in Escherichia coli BL21(DE3)
-
expression of wild-type and mutant K177A in Bacillus megaterium as His-tagged proteins
-
expression of wild-type from plasmid in deficient mutant ceg1-25
-
expression of wild-type full length enzyme and catalytic domain in deficient Saccharomyces cerevisiae strain YBS2 as His-tagged protein, functional complementation by both of them
gene A103R, expression of His6-tagged A103R protein in Escherichia coli strain BL21(DE3)
-
gene CEG1 from genomic library, expression of catalytically active alpha-subunit in Escherichia coli, chromosome mapping
-
gene CEG1, guanylylpeptide, DNA and amino acid sequence determination
-
gene L2, expression of wild-type and mutant K226A protein lambda2 in Trichoplusia ni or Spodoptera frugiperda SF21 cells via baculovirus infection
-
in vitro translation of wild-type and mutant enzyme
mRNA capping enzyme, DNA sequence determination and analysis
mutant lacking the C-terminal domain, consisting of the D1R domain, residues 1-545, expression in Escherichia coli BL21(DE3)pLysS
-
overexpression of LEF-4 subunit in Spodoptera frugiperda cells by recombinant virus infection under control of polyhedrin promotor
-
RNA genome sequence determination and analysis, expression of lambdaC in Spodoptera frugiperda cells via baculovirus infection
-